Sorghum dwarfing mutation breeding method
A technology of mutation breeding and chemical mutagen, applied in botany equipment and methods, seed immunity, plant gene improvement, etc., to achieve the effect of expanding mutation spectrum, accelerating breeding process, and excellent germplasm resources
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[0026] A sorghum dwarf mutation breeding method, comprising the following steps:
[0027] Step 1: Pretreatment after seed selection;
[0028] Step 2: physical mutagenesis, putting the sorghum seeds in step 1 into plastic bags for irradiation treatment;
[0029] Step 3: chemical mutagenesis, soaking the seeds irradiated in step 2 in a chemical mutagen for treatment to obtain chemically mutagenized seeds;
[0030] Step 4: post-mutation treatment;
[0031] Step five: screening of dwarf sorghum mutants;
[0032] Step 6: All the M1 generation seeds harvested by a single plant are planted in the next year to form M2 generation lines, and untreated seeds are selected as controls, and the flowering period is compared with the control, and the stability of the flowering period traits is observed in the strains. When it tends to be stable, carry out mixed harvesting and detachment, harvest the M3 generation seeds, and obtain the short-stalked mutant sorghum varieties; continue to pla...
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