Resistance identification method of sweet potato scab for stem tip vegetables

A resistance identification and scab technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of increasing the risk of pesticide residues and environmental pollution, increasing labor costs, etc., to achieve the goal of increasing pesticide residues and environmental protection Risk of contamination, increased labor costs, effects of stable results

Pending Publication Date: 2021-03-19
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0028] 1. Although studies have shown that pesticide control can significantly inhibit the occurrence of sweet potato scab, the use of these pesticides not only increases labor costs, but also increases the risk of pesticide residues and environmental pollution, and is especially not suitable for use on sweet potatoes for stem tip vegetables

Method used

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  • Resistance identification method of sweet potato scab for stem tip vegetables
  • Resistance identification method of sweet potato scab for stem tip vegetables
  • Resistance identification method of sweet potato scab for stem tip vegetables

Examples

Experimental program
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Effect test

Embodiment 1

[0061] The invention provides a method for identifying resistance to sweet potato scab for stem apex vegetables, comprising the following steps:

[0062] S1. Preparation of sweet potato materials to be identified: Select 10 sweet potato varieties or strains for stem tip vegetables such as Fucai 13-14, Guangcaishu No. 7 and Fucaishu 25, and plant them in Zhongluotan Baiyun Base of Guangdong Academy of Agricultural Sciences. According to the observation of the traits, the growth is healthy and disease-free, and it will be used after multiplication.

[0063] S2. Preparation and activation of pathogenic bacteria: activate the sweet potato scab strain CJ1 stored in the Crop Research Institute of Guangdong Academy of Agricultural Sciences, inoculate it on a PDA medium plate, and cultivate it under dark conditions at 25°C. After the mycelium grows on the PDA medium plate , for inoculum preparation.

[0064] S3. Preparation of inoculum: S2 activated pathogenic bacteria were propagate...

Embodiment 2

[0087] Utilize the method of the present invention to carry out disease-resistant identification to 28 parts of stem tip vegetable sweet potato varieties or strain materials, comprise the following steps:

[0088] S1. Preparation of sweet potato materials to be identified: Select 28 varieties or strains of sweet potato varieties or strains for stem tip vegetables, including Guangcaishu 11-52, Qiancaishu No. 2 and Fucaishu 18, and plant them in Zhongluotan Baiyun Base, Guangdong Academy of Agricultural Sciences , through the observation of traits, the growth is healthy and disease-free, and it will be used after multiplication.

[0089] S2. Preparation and activation of pathogenic bacteria: activate the sweet potato scab strain CJ1 stored in the Crop Research Institute of Guangdong Academy of Agricultural Sciences, inoculate it on a PDA medium plate, and cultivate it under dark conditions at 25°C. After the mycelium grows on the PDA medium plate , for inoculum preparation.

[...

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Abstract

The invention discloses a resistance identification method of sweet potato scab for stem tip vegetables, relates to the technical field of resistance identification of sweet potato scab, and specifically relates to the resistance identification method of sweet potato scab for stem tip vegetables. The method comprises the following steps: S1, preparing a sweet potato material to be identified; S2,preparing and activating pathogenic bacteria; S3, preparing a disease inoculum; S4, disease nursery induction and artificial inoculation; and S5, disease resistance evaluation: after the susceptible control variety Guangdong sweet potato No.5 is completely attacked, starting to investigate the attack situation, calculating the disease index, and carrying out resistance evaluation according to thedisease index. The invention provides the resistance identification method of sweet potato scab for stem tip vegetables. The method is reliable, stable in result, and suitable for identifying and evaluating the resistance of sweet potato scab, by combining disease nursery induction with a spore suspension artificial inoculation method, and utilizing a control variety with stable anti-infection phenotype.

Description

technical field [0001] The invention relates to the technical field of resistance identification of sweet potato scab, in particular to a resistance identification method of sweet potato scab for stem tip vegetables. Background technique [0002] Sweet potato is an important food, feed, industrial raw material and bioenergy crop in the world. At the same time, the young stems and petioles of sweet potatoes have become popular vegetable crops in more and more areas of China because of their rich nutrition and delicious taste. A new vegetable-specific sweet potato variety with tender shoots or petioles as edible parts. Leafy vegetables are most scarce in summer in southern my country. Guangcaishu 5 and Fushu 7-6 are important sources of leafy vegetables. However, these varieties often suffer from scab infection during production. [0003] The existing sweet potato scab is one of the important diseases in the southern potato area of ​​my country. The pathogenic bacterium is C...

Claims

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Application Information

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IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 黄立飞张新新房伯平陈景益
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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