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In-vitro propagation method of plant Hydrocera triflora

A technology of in vitro propagation and water horn, which is applied in botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems of low seed vigor and few seeds, and achieve simple formula, protection of germplasm resources, and ingredients Effects from a wide range of sources

Active Publication Date: 2018-11-30
海南省农业科学院热带园艺研究所
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Problems solved by technology

[0005] (1) In the cultivation of Impatiens flower resources, it seldom produces seeds or the seed vigor is low, therefore, vegetative propagation becomes its main mode of propagation;

Method used

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  • In-vitro propagation method of plant Hydrocera triflora

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Embodiment Construction

[0031] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0032] The application principle of the present invention will be further described below in conjunction with the accompanying drawings.

[0033] Such as figure 1 Shown, the in vitro propagation method of the plant water horn that the embodiment of the present invention provides comprises the following steps:

[0034] S101, collection and disinfection of seeds, collecting mature seeds, and storing them in a refrigerator at a temperature of 3-4°C for later use. Select healthy and plump Shuijiao seeds for disinfection;

[0035] S102, seed germination and bud induction culture: culture temperature 24±2°C, light time 9h / d, ...

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Abstract

The invention belongs to a new plant or a method for obtaining a new plant and the technical field of plant regeneration by tissue culture technology, and discloses an in-vitro propagation method of plant Hydrocera triflora. The in-vitro propagation method of the plant Hydrocera triflora comprises seed collection and disinfection; seed germination and bud induction culture; bud proliferation subculture; rooting and strong seedling culture. The method has great advantages: 1. a small amount of seeds are needed; 2. the proliferation coefficient is large, and the bud proliferation coefficient reaches 8.00 in 30 days of culture; 3. rooting and strong seedlings are good, the rooting rate is 100%, and the number of roots is as high as 6.42; 4. a developed medium has a simple formula and a wide range of ingredients, and is easy to operate and formulate; 5. the breeding of seedlings can be produced annually without being limited by time and seasons.

Description

technical field [0001] The invention belongs to the field of new plants or methods for obtaining new plants; the technical field of plant regeneration through tissue culture technology, and in particular relates to a method for in vitro propagation of plant water horns. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: At present, the commonly used prior art in the industry is as follows: Hydrocera triflora (L.) Wight. et Arn. is a perennial aquatic herb under the genus Hydrocera of the family Impatiens, and is a single genus and single species of plant. Hainan is currently the only place where Shuijiao is distributed in China, and Shuijiao is listed as a regionally extinct (RE) plant. The water corner has great scientific research value, horticultural value and ecological value. Since the water corners are only sporadically distributed and are in a primitive state of self-destruction, there has been no protec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王景飞戚华沙任军方符瑞侃黄赛吕德任梁定民
Owner 海南省农业科学院热带园艺研究所
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