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Method for inducing callus of lycoris radiate by using flower stalks

A technology of callus and lycoris, applied in the field of inducing callus of lycoris by pedicels, can solve the problems of high risk, achieve the effect of alleviating the high pollution rate and improving the success rate of callus induction

Inactive Publication Date: 2013-09-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of the invention uses Lycoris bulbs as explants to induce culture to produce Lycoris. This method needs to be at the cost of losing Lycoris bulbs. For Lycoris germplasm resources that are gradually scarce, this method is more risky

Method used

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  • Method for inducing callus of lycoris radiate by using flower stalks
  • Method for inducing callus of lycoris radiate by using flower stalks
  • Method for inducing callus of lycoris radiate by using flower stalks

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Experimental program
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Effect test

Embodiment 1

[0022] 1) The preparation of culture medium, comprises basic culture medium and callus induction culture medium, each component and the content per liter are respectively:

[0023] ①Basic medium: MS (Murashige and Skoog 1962), add 30g sucrose, 8g agar, adjust pH to 5.7;

[0024] ②Induction medium: MS+2,4-D (2,4 dichlorophenoxyacetic acid) 2 mg, BA (benzylaminoadenine) 2 mg.

[0025] 2) Preparation of explants: Pick the young inflorescences of Lycoris (inflorescences are not unfolded), and store them in a refrigerator at 4°C; take them out one day before inoculation, and place them in clean water containing detergent and Tween 80 in a volume ratio of 1:1 After soaking in medium for 15 minutes, rinse with water for 3 hours, and then disinfect with ethanol with a volume ratio of 75% for 10 minutes, soak in mercuric chloride solution with a volume ratio of 0.1% for 10 minutes, and rinse with sterile water for 4-5 times to obtain sterile inflorescences; cut off the ovary The pedic...

Embodiment 2

[0032] 1) The preparation of culture medium, comprises basic culture medium and callus induction culture medium, each component and the content per liter are respectively:

[0033] ①Basic medium: MS (Murashige and Skoog 1962), add 30g sucrose, 8g agar, adjust pH to 5.6;

[0034] ②Induction medium: MS+2, 4-D 2mg, BA 2.5mg.

[0035] 2) Preparation of explants: Pick the young inflorescences of Lycoris (inflorescences are not unfolded), and store them in a refrigerator at 4°C; take them out one day before inoculation, and place them in clean water containing detergent and Tween 80 in a volume ratio of 1:1 After soaking in medium for 15 minutes, rinse with water for 3 hours, then disinfect with 75% ethanol by volume for 12 minutes, soak in mercuric chloride solution with 0.1% volume for 12 minutes, rinse with sterile water for 4-5 times to obtain sterile inflorescences; cut off the ovary The pedicel part from the bottom to the top of the flower stem is cut into 2-3mm small section...

Embodiment 3

[0041] 1) The preparation of culture medium, comprises basic culture medium and callus induction culture medium, each component and the content per liter are respectively:

[0042] ①Basic medium: MS (Murashige and Skoog 1962), add 30g sucrose, 8g agar, adjust pH to 5.8;

[0043] ②Induction medium: MS+2, 4-D 2.5mg, BA 2mg.

[0044] 2) Preparation of explants: Pick the young inflorescences of Lycoris (inflorescences are not unfolded), and store them in a refrigerator at 4°C; take them out one day before inoculation, and place them in clean water containing detergent and Tween 80 in a volume ratio of 1:1 After soaking in medium for 15 minutes, rinse with water for 3 hours, and then disinfect with ethanol with a volume ratio of 75% for 10 minutes, soak in mercuric chloride solution with a volume ratio of 0.1% for 12 minutes, and rinse with sterile water for 4-5 times to obtain sterile inflorescences; cut off the ovary The pedicel part from the bottom to the top of the flower stem...

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Abstract

The invention discloses a method for callus of lycoris radiate by using flower stalks, comprising the following steps: taking the flower stalks of the lycoris radiate after being sterilized; inoculating in induction culture medium, cultivating for 28-32 d in sunshine, then moving to a dark environment, and cultivating for 88-92 d, wherein the induction culture medium comprises 2.0-2.5 mg / L of 2,4-D and 1.5-2.5 mg / LBA of MS culture medium, and the MS culture medium comprises sugarcane of which concentration is 30-60 g / L and agar of which concentration is 5-8 g / L and pH is 5.6-5.8. The invention adopts the flower stalks of the lycoris radiate as the exophyte, produces the callus of the lycoris radiate through cultivating in the induction culture medium, cannot be at the cost of consuming the bulbs, and effectively protects the genetic resources of the rare lycoris radiate which has good quality.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing lycoris callus by using pedicel. Background technique [0002] Lycoris radiata is a famous bulbous flower native to my country, which has both ornamental and medicinal value. The natural reproduction rate of Lycoris is low, and the juvenile period of bulbs is as long as 5-6 years. In recent years, due to the excavation of the medicinal value of Lycoris, the resources of Lycoris have been collected in large quantities, and the protection of wild resources is facing a crisis. [0003] In 1979, Koyama first reported the tissue culture of Lycoris, and the research on tissue culture of Lycoris plants in my country has been more than 20 years. The domestic research mainly focuses on exploring different explants of Lycoris, such as bulbs, bulbs, embryos, etc. to induce Plantlets were obtained from adventitious buds or callus. Inducing adventitious bu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈菁珏夏宜平常乐肖郁绵吴昀
Owner ZHEJIANG UNIV
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