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A kind of centella asiatica polyploid and its induced breeding method

A centella asiatica and polyploid technology is applied in the field of centella asiatica polyploid and its induced breeding, which can solve the problems of high toxicity of colchicine, high price of colchicine, and high operation requirements of experimenters, and achieves high performance. Stability and reproducibility, improving the success rate of induction, the effect of high success rate of induction

Active Publication Date: 2017-11-24
GUILIN SANJIN PHARMACEUTICALS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, colchicine-induced polyploids have insurmountable shortcomings: colchicine is highly toxic and pollutes the environment; colchicine is expensive and expensive to use in experiments, so it is necessary to seek low-pollution, low-cost, low-toxicity induction Induced chromosome doubling
This application includes a method for inducing and cultivating Centella asiatica species in the laboratory, which needs to be completed in the cultivation room, and has the disadvantages of high requirements for experimenters, cumbersome steps, and high labor costs
Moreover, the content of the active substance in the prepared polyploid variety was not detected in the patent application, and the experimental results were inaccurate

Method used

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  • A kind of centella asiatica polyploid and its induced breeding method
  • A kind of centella asiatica polyploid and its induced breeding method
  • A kind of centella asiatica polyploid and its induced breeding method

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Experimental program
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Effect test

Embodiment 1

[0036] A method for breeding Centella asiatica polyploidy, comprising the following steps:

[0037] 1. Transplant wild Centella asiatica into the culture medium; the temperature for transplanting wild Centella asiatica is 12-15°C; the humidity is 80%, the medium is the cultivation substrate of medicinal dregs, and compound fertilizer is added as the base fertilizer. The medicinal dregs cultivation medium added with compound fertilizer contains 1000-1200mg / kg of available N, 300-350mg / kg of available P, 6000-6500mg / kg of available K, 300-350g / kg of organic matter, 25-35cmol / kg of exchangeable Ca, Exchangeable Mg 1.5-3cmol / kg, effective Zn 40-50mg / kg, effective Fe 300-320mg / kg, effective B 0.1-0.4mg / kg; pH 6.0-6.5.

[0038] 2. Prepare an inducer solution; the inducer solution has a concentration of 30mg / L amisulfame, and is added with 2wt% dimethyl sulfoxide;

[0039] 3. Wrap the absorbent cotton on the growth point of Centella asiatica cluster leaves, soak the absorbent cotton...

Embodiment 2

[0042] A method for breeding Centella asiatica polyploidy, comprising the following steps:

[0043] 1. Transplant wild Centella asiatica into the culture medium; the temperature for transplanting wild Centella asiatica is 12-16° C.; the humidity is 70-75%. The culture medium is the dregs cultivation substrate, and compound fertilizer is added as the base fertilizer. The medicinal dregs cultivation medium added with compound fertilizer contains 1000-1200mg / kg of available N, 300-350mg / kg of available P, 6000-6500mg / kg of available K, 300-350g / kg of organic matter, 25-35cmol / kg of exchangeable Ca, Exchangeable Mg 1.5-3cmol / kg, effective Zn 40-50mg / kg, effective Fe 300-320mg / kg, effective B 0.1-0.4mg / kg; pH 6.0-6.5.

[0044] 2. Prepare an inducer solution; the inducer solution has a concentration of 40mg / L amisulfame, and is added with 2wt% dimethyl sulfoxide.

[0045] 3. Wrap the absorbent cotton on the growing point of Centella asiatica clumped leaves, soak the absorbent cott...

Embodiment 3

[0048] A method for breeding Centella asiatica polyploidy, comprising the following steps:

[0049] 1. Transplant wild Centella asiatica into the culture medium; the temperature for transplanting wild Centella asiatica is 12-18°C; the humidity is 75%, the medium is the cultivation substrate of medicinal dregs, and compound fertilizer is preferably added as the base fertilizer. The medicinal dregs cultivation medium added with compound fertilizer contains 1000-1200 mg / kg of available N, 300-350 mg / kg of available P, 6000-6500 mg / kg of available K, 300-350 g / kg of organic matter, and 25-35 cmol of exchangeable Ca / kg, exchangeable Mg 1.5~3cmol / kg, effective Zn 40~50mg / kg, effective Fe 300~320mg / kg, effective B 0.1~0.4mg / kg; pH 6.0~6.5.

[0050] 2. Prepare an inducer solution; the inducer solution has a concentration of 50 mg / L amisulfame, and is added with 3wt% dimethyl sulfoxide.

[0051] 3. Wrap the absorbent cotton on the growing point of Centella asiatica bushy leaves, soak...

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Abstract

The invention relates to the field of plant breeding, in particular to a centella asiatica polyploid and a method for inducing breeding thereof. The method comprises the following steps: transplanting wild centella asiatica to the culture medium; preparing an inducer solution; the inducer solution has a concentration of 30-50 mg / L amisulfame, and is added with 1-3 wt % dimethyl sulfoxide ; Wrap the absorbent cotton on the growing point of Centella asiatica cluster leaves, soak the absorbent cotton with inducer solution; treat it under natural light conditions for 2 to 4 days; use asexual reproduction to expand and preserve the samples that are tetraploid detected by chromosome microscope. The invention conducts in vivo induction of Centella asiatica, not only has a high induction transformation rate, but also greatly increases the content of medicinal active substances in the multi-volume Centella asiatica.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a centella asiatica polyploid and a method for inducing breeding thereof. Background technique [0002] Centella asiatica is a plant of the genus Centella asiatica of the Umbelliferae family. There are about 20 species in the world. Only one species of Centella asiatica is produced in my country, which is an important Chinese herbal medicine. Thousands of years of history. Widely distributed in various counties and cities in Guangxi, it is one of the Zhuang medicines with Guangxi national characteristics. [0003] The medicinal materials of Centella asiatica mainly come from the mining of wild resources, and the quality controllability is poor. Digging will inevitably result in the destruction of Centella asiatica habitat and wild populations. Therefore, the implementation of GAP (Good Agricultural Practice) of Chinese medicinal materials is of great significance to ensure the qua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
Inventor 邹节明蒋水元钟小清
Owner GUILIN SANJIN PHARMACEUTICALS CO LTD
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