Induction culture medium of autograph tree and vegetative propagation method for autograph tree

A technique of induction medium and asexual reproduction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low utilization rate of female parent, unstable reproduction, unfavorable large-scale production of factories, etc.

Inactive Publication Date: 2020-10-30
FOSHAN YUESHAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the common propagation methods of signature trees are cuttings and sowing. Sowing belongs to sexual reproduction, and its reproduction has strong instability. It is difficult to maintain the excellent characteristics of the parents, which is not conducive to large-scale industrial production.
Cuttings belong to asexual reproduction. Although the new individuals produced can be exactly the same as the mother body without change, the cuttings do more damage to the mother body, and the utilization rate of the mother body is not high, which is also not conducive to large-scale factory production. Therefore, A new breeding method is needed for factory production

Method used

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  • Induction culture medium of autograph tree and vegetative propagation method for autograph tree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of the signature tree induction medium of the present invention

[0024] According to the formula shown in Table 1 and Table 2, weigh the MS medium and the components of the signature tree induction medium of the present invention respectively, place them in a conical flask, add an appropriate amount of distilled water, stir to dissolve, and adjust the volume to 1 L. After the pH value reaches 5.8, it is divided into tissue culture bottles, sealed and placed in an autoclave, sterilized at 121°C for 20 minutes, cooled and solidified, and the signature tree induction medium of the present invention is obtained.

[0025] The composition and consumption thereof of table 1 improved MS culture medium of the present invention

[0026]

[0027] Table 2 The composition and consumption amount of signature tree induction medium of the present invention

[0028] Element Final concentration MS medium - 6-Bianaminoadenine (6-BA) 0.5m...

Embodiment 2

[0029] Embodiment 2: method for inducing and cultivating young leaves of signature tree

[0030] A signature tree asexual propagation method, it comprises the following steps:

[0031] Step S10: Pick the tender leaves of the top 2-4 pieces of the top of the signature tree plant with good varieties and health as explants, soak them in alcohol with a mass concentration of 75% for 26 seconds, rinse them with sterile water, and place them in a mass Sterilize in 0.1% mercuric chloride solution for 6 minutes, then rinse with sterile water;

[0032] Step S20: In the ultra-clean workbench, cut off the petiole of the sterilized young leaves of the signature tree. If a wound is formed on the young leaves before or during the disinfection, part of the wound is also cut off, and then a scalpel is used to remove the petiole. Gently scrape 4 times on one side of the plant leaf, and cut the leaf into several small pieces along the veins;

[0033] Step S30: Take the blade small piece obtain...

Embodiment 3

[0035] Example 3: Effect test of signature tree induction medium

[0036] Referring to the method for inducing and culturing young leaves of the signature tree described in Example 2, the explants of the signature tree were respectively inoculated in the medium shown in Table 3, and after 45 days of induction culture, the browning rate, Induction rate and induction time, the results are shown in Table 4.

[0037] Table 3 Signature Tree Induction Medium

[0038]

[0039] In the above experimental group and control group, MS medium was used as the basic medium, and 6g / L agar powder and 28g / L white granulated sugar were added, and other ingredients were added according to Table 3.

[0040] Table 4 The test results of the signature tree induction medium

[0041] serial number Induction rate a(%) Average induction time b (days) Browning rate c(%) Comprehensive score Control group 1 63.16 34.14 8.62 38.562 Test group 1 30.21 44.05 24.37 6.079 ...

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Abstract

The invention discloses an induction culture medium of an autograph tree and a vegetative propagation method for the autograph tree. According to the induction culture medium of the autograph tree, anMS culture medium serves as a basal culture medium, and 6-benzylaminopurine, naphthylacetic acid, silver nitrate, activated carbon, manganese dioxide, hydrolyzed casein, agar powder and white granulated sugar are added. The vegetative propagation method comprises the steps of firstly, carrying out disinfecting treatment on an explant, then, carrying out slight scraping treatment and scratching treatment, and finally, carrying out inoculating. The induction culture medium of the autograph tree and the corresponding vegetative propagation method, disclosed by the invention, can be used for promoting growth and development of tender leaves of the autograph tree, shortening induction time, reducing a browning phenomenon and increasing the survival rate of induction of the tender leaves of theautograph tree.

Description

technical field [0001] The invention relates to the technical field of signature tree cultivation, in particular to a signature tree induction medium and a signature tree asexual propagation method. Background technique [0002] Signature tree, also known as Clusia wood, is a woody foliage plant belonging to the family Garcinia. The seedling stems are slightly fleshy, the leaves are oval, with blunt apex, short apex, blunt base, smooth and flat leathery leaves, dark green and shiny leaves, and emerald green petioles. They are generally used as horticultural ornamental plants because The leaves can be signed, so it is called a signature tree. [0003] At present, the commonly used propagation methods of signature trees are cuttings and sowing. Sowing belongs to sexual reproduction, and its reproduction is highly unstable. It is difficult to maintain the excellent characteristics of the parents, which is not conducive to large-scale industrial production. Cuttings belong to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 周国权沐潇潇郑彬江郑彬旭张林杰
Owner FOSHAN YUESHAN BIOLOGICAL TECH
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