Tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima

Abstract

The invention discloses a tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima. According to the method, the improved MS, 6-benayl aminopurine (6-BA) and 3-indoleacetic acid (IAA) are used as an inductive culture medium for the cluster buds of Chongzuo camellia nitidissima; and the combination of the two growth hormones can be used for activating acting enzymes in the body cells of Chongzuo camellia nitidissima, and promoting the germination and the growth of the explants of Chongzuo camellia nitidissima in a proper concentration and a weakly acidic environment; the thick and strong cluster buds can be formed after the culturing for 55 days, and the propagation rate is increased by 20 times. According to the method disclosed by the invention, 1 / 2 improved MS and indolebutyric acid (IBA) or 1 / 2 improved MS and napthaleneacetic acid (NAA) are further used as an inductive culture medium for rooting, and capable of effectively promoting the single seedlings of Chongzuo camellia nitidissima to root to form new plants. Via the tissue culture and propagation method applying the three inductive culture mediums for Chongzuo camellia nitidissima, Chongzuo camellia nitidissima can be rapidly and effectively propagated, thus solving the problems that wild resources are diminished gradually and the natural propagation rate of Chongzuo camellia nitidissima is low, and effectively protecting the precious plant genetic resource which is rare in the world.

Description

Technical field [0001] The invention relates to the field of plant tissue culture and propagation, in particular to a method for tissue culture and propagation of Chongzuo Camellia tea and an induction medium. Background technique [0002] C.chungtsoensis SYLiang et LDHuang, an evergreen shrub, is a recently discovered rare variety of camellia. It is the second variety of camellia that blooms in four seasons after azalea red camellia. , It is not only the brightest yellow in the Camellia family, but it also blooms throughout the year. Chongzuo Jinhua tea is only distributed in a small amount in the local limestone low mountain area of ​​Chongzuo, Guangxi. This species is similar to the lemon golden flower tea, the difference is that the flower is large and dark yellow, the petals are 13-16, and it blooms in all seasons. Chongzuo Golden Camellia has evenly distributed flowers and dense branches. It can grow normally under full light conditions. It has high ornamental value and h...

AI Technical Summary

Problems solved by technology

The natural distribution range of Chongzuo golden camellia is narrow and the quantity is limited. Due to the thin and weak branches, the low rooting rate of cuttings, and the difficulty of asexual reproduction, it is difficult to meet the market demand of garden flowers.

So far, although there are some reports on the plant tissue culture of Camellia japonica at home and abroad, there is no report on the tissue culture of Camellia japonica in Chongzuo