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Lepista sordida and separation and propagation method and soil covering cultivation method thereof

The technology of a flower face mushroom and its cultivation method is applied in the field of edible fungus research, which can solve the problems affecting the commercialization of the fungus, low biological transformation rate, immature cultivation technology, etc., and achieve green ecological cultivation, good ventilation, and no The effect of perishability

Active Publication Date: 2018-07-20
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on A. variegata has mainly focused on biological characteristics, strain isolation and identification, strain cultivation, nutritional components and functional evaluation, etc., because of its small number of cultivated strains, low biological transformation rate, and immature cultivation techniques. and other factors seriously affect the commercialization of the bacteria

Method used

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  • Lepista sordida and separation and propagation method and soil covering cultivation method thereof
  • Lepista sordida and separation and propagation method and soil covering cultivation method thereof
  • Lepista sordida and separation and propagation method and soil covering cultivation method thereof

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preparation example Construction

[0033] The preparation method of the cultivar compost described in step (4) is to mix the raw materials in the proportion of the formula evenly, autoclave at 121°C for 1.5-2.0 hours, and control the water content at 60-70%.

[0034] The plastic bag described in the step (4) is a polypropylene plastic bag with a size of 17cm×33cm×0.05cm, and the loading amount of the cultivar compost is 0.85-0.90kg / bag.

[0035] The soil-covering cultivation method of the fragrant mushroom of the present invention comprises the following steps:

[0036] 1) Select the plot: choose the monsoon evergreen broad-leaved forest as the soil-covered cultivation plot. The altitude of the plot is 2100-2200m, the temperature is 16-28°C, the air humidity is 65%-90%, and the scattered light is sufficient. Add the lime powder of 1% of soil gross weight in block and carry out disinfection treatment to soil, and select the surface layer soil under the forest to be covering soil material, and described surface l...

Embodiment 1

[0044] A method for separating and propagating the mushroom, its separation and multiplication, and its soil-covering cultivation method, comprising the following steps:

[0045] (1) Strain isolation, cultivation, identification and preservation

[0046] A fruiting body of wild Amanita variegata collected by hand was used as a domestication material, and the N006# strain of Amanita variegata was obtained by tissue separation method, and then its mycelium was inoculated on the slant of agar medium in a test tube, and cultured at 25°C for 7 days to obtain Test tube strains were stored in a refrigerator at 4°C for later use. The formula of described test tube agar medium is improved PDA medium, formula is: potato 200g / L, glucose 20g / L, agar 15g / L, peptone 1g / L, yeast extract 1g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L.

[0047] ——Identification of Lepista sordida (Schumach.) Singer N006#

[0048] 1. The fruiting body shape of the N006# strain of the...

Embodiment 2

[0086]A method for separating and multiplying Lepista sordida (Schumach.) Singer N006# and a soil-covering cultivation method, comprising the following steps:

[0087] 1) Isolation and cultivation of strains: the fruiting bodies of wild Amanita variegata were used as domestication materials, and the N006# strain of Amanita variegata was obtained by tissue separation method, and then its mycelium was inoculated on the slant of the improved PDA medium, at 25°C Cultivate for 10 days to obtain test tube strains, and store them in a refrigerator at 6°C for later use; the formula of the improved PDA medium is: potato 200g / L, glucose 20 g / L, agar 15g / L, peptone 1 g / L, yeast Paste 1 g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L.

[0088] 2) Propagation of the mother species of the N006# strain of A. japonica: pick the mycelia from the test tube strain and transfer it to the improved PDA medium (the formula is as above), the culture condition is 25°C constant t...

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Abstract

The invention discloses lepista sordida and a separation and propagation method and soil covering cultivation method thereof. N006# strains are obtained by tissue separation of wild lepista sordida fruiting bodies, and the lepista sordida N006# strains are preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation date is June 1st, 2016; the preservation registration number is CGMCC NO. 12507. The lepista sordida N006# strains are obtained through tissue separation, a propagation method of the strains is mastered, and strain guarantees are provided for artificial cultivation of lepista sordida. The lepista sordida fruiting bodies are obtained through artificial soil covering cultivation, germplasm resources of this specie are protected, and a novel edible fungus variety is developed.

Description

technical field [0001] The invention belongs to the technical field of edible fungus research, and in particular relates to a method for the isolation and identification, propagation and multiplication of original species, multiplication of cultivated species and soil-covering cultivation method of Amanita chinensis and its strains. Background technique [0002] Lepista sordida (Schumach.) Singer is mainly distributed in Yunnan in western Yunnan, northwestern Yunnan and central Yunnan. It usually occurs in July and August. It has a strong taste and bright purple color. It is a nutritious and Rare and high-quality wild edible fungi with various pharmacological effects have good economic benefits. In recent years, the research on A. variegata has mainly focused on biological characteristics, strain isolation and identification, strain cultivation, nutritional components and functional evaluation, etc., because of its small number of cultivated strains, low biological transform...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40C12N1/02C12N1/14C12R1/645
CPCC12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 桂明英郭相岳万松吴素蕊马明杨珍福
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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