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A kind of in vitro propagation method of plant water horn

A technology of in vitro propagation and water horns, which is applied in botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems of few seeds produced and low seed vigor, and achieve the protection of germplasm resources, simple formula, and rooting Good seedling effect

Active Publication Date: 2021-04-30
海南省农业科学院热带园艺研究所
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Problems solved by technology

[0005] (1) In the cultivation of Impatiens flower resources, it seldom produces seeds or the seed vigor is low, therefore, vegetative propagation becomes its main mode of propagation;

Method used

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  • A kind of in vitro propagation method of plant water horn

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Embodiment Construction

[0031] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0032] The application principle of the present invention will be further described below in conjunction with the accompanying drawings.

[0033] Such as figure 1 Shown, the in vitro propagation method of the plant water horn that the embodiment of the present invention provides comprises the following steps:

[0034] S101, collection and disinfection of seeds, collecting mature seeds, and storing them in a refrigerator at a temperature of 3-4°C for later use. Select healthy and plump Shuijiao seeds for disinfection;

[0035] S102, seed germination and bud induction culture: culture temperature 24±2°C, light time 9h / d, ...

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Abstract

The invention belongs to new plants or methods for obtaining new plants; in the technical field of plant regeneration through tissue culture technology, a method for in vitro propagation of plant water horns is disclosed, and the in vitro propagation method for plant water horns comprises: Collection and disinfection; seed germination and bud induction culture; bud proliferation subculture; rooting and strong seedling cultivation. The invention has great advantages: 1. A small amount of seeds are needed; 2. The multiplication coefficient is large, and the bud multiplication coefficient reaches 8.00 after 30 days of cultivation; 3. The rooting and strong seedlings are good, the rooting rate is 100%, and the number of roots is as high as 6.42; 4. The formula of the developed medium is simple, the source of ingredients is wide, and it is easy to operate and prepare; 5. The breeding of seedlings is not limited by time and season, and can be produced every year.

Description

technical field [0001] The invention belongs to the field of new plants or methods for obtaining new plants; the technical field of plant regeneration through tissue culture technology, and in particular relates to a method for in vitro propagation of plant water horns. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: At present, the commonly used prior art in the industry is as follows: Hydrocera triflora (L.) Wight. et Arn. is a perennial aquatic herb under the genus Hydrocera of the family Impatiens, and is a single genus and single species of plant. Hainan is currently the only place where Shuijiao is distributed in China, and Shuijiao is listed as a regionally extinct (RE) plant. The water corner has great scientific research value, horticultural value and ecological value. Since the water corners are only sporadically distributed and are in a primitive state of self-destruction, there has been no protec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王景飞戚华沙任军方符瑞侃黄赛吕德任梁定民
Owner 海南省农业科学院热带园艺研究所
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