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Method for long-term storage of berberis heteropoda seeds

A technology for long-term preservation of barberry black fruit, applied in the direction of plant preservation, botanical equipment and methods, applications, etc., can solve the problems of destroying seed cell structure, unfavorable seed preservation, etc., and achieve the effect of inhibiting the crystallization process

Inactive Publication Date: 2017-04-05
FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the seeds are directly placed in liquid nitrogen without cryoprotection, the cell structure of the seeds will be destroyed. At the same time, even if the seeds are dried in a desiccant and then exposed to the air again, they will return to moisture, and the preservation of the seeds will not be used.

Method used

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  • Method for long-term storage of berberis heteropoda seeds
  • Method for long-term storage of berberis heteropoda seeds
  • Method for long-term storage of berberis heteropoda seeds

Examples

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Effect test

Embodiment 1

[0023] A method for long-term preservation of barberry seeds, comprising the steps of:

[0024] Disinfection and sterilization: Take out the exocarp of healthy barberry seeds collected in the Banfanggou Branch of the State-owned Forest Administration Bureau in Eastern Tianshan, Xinjiang, and then soak them in carbendazim soaking solution for 45 minutes after drying naturally. For every 8 grams of 50% carbendazim wettable powder, mix 2L of water. Spread the seeds on a mesh screen with a wooden frame to a thickness of 1 cm. Add sulfur powder into the sulfur fumigator, and fumigate the net-dried seeds for 1 hour. Fumigation once every 5 days, continuous fumigation 2 times.

[0025] Seed drying: place the sterilized Berberis nigrum seeds on a seed drying box to dry for 5 days, turning them every 1 hour. The seed airing box is surrounded by a wooden frame with a width of 2 cm, a height of 3.5 cm, and a length of 2.5 meters. The bottom of the airing box is an 8-purpose high-densi...

Embodiment 2

[0031] A method for long-term preservation of barberry seeds, comprising the steps of:

[0032] Disinfection and sterilization: Take out the exocarp of healthy barberry seeds collected in Xiaodonggou Branch of Xinjiang Altai Mountains State-owned Forest Administration Bureau, take out the exocarp, and soak them in carbendazim soaking solution for 60 minutes after drying naturally. For every 10 grams of 50% carbendazim wettable powder, mix 2.5 L of water. Spread the seeds on a mesh screen with a wooden frame to a thickness of 1.5 cm. Add sulfur powder into the sulfur fumigator, and fumigate the net-dried seeds for 1 hour. Fumigation once every 7 days, continuous fumigation 2 times.

[0033] Seed drying: place the sterilized Berberis nigrum seeds on a seed drying box to dry for 5 days, turning them every 1 hour. The seed airing box is surrounded by a wooden frame with a width of 2 cm, a height of 3.5 cm, and a length of 2.5 meters. The bottom of the airing box is an 8-purpose...

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Abstract

The invention discloses a method for long-term storage of berberis heteropoda seeds. The method comprises the following steps that the seeds are sterilized, disinfected and aired, and the dried berberis heteropoda seeds are placed under a 2-5 DEG C condition and soaked in a mixed solution comprising 15% of ethanediol, 10% of dimethyl sulfoxide (MSO) and 10% of cane sugar for 45-60 minutes; prefrozen berberis heteropoda seeds are placed into an absorbent gauze bag, and the berberis heteropoda seeds and the gauze bag are placed into a dryer with the lower layer being made of silica gel for drying; and the dried seeds are uniformly smeared with ceresin, packaged with a tinfoil bag airtightly, placed into a low-temperature environment with the temperature being 25-40 DEG C to be stored for 30-60 minutes, and then packed into a sealed bag which is filled with liquid nitrogen and has the temperature of -196 DEG C for cryopreservation. By adoption of the method, the activity of the seeds can be preserved in a long term, the rate of emergence of the seeds is guaranteed and increased, and the seedling establishment capability is improved.

Description

technical field [0001] The invention relates to the field of seed preservation, in particular to a method for long-term preservation of barberry seeds. Background technique [0002] Berberis atrocarpa (scientific name: Berberis atrocarpa) is a plant of the genus Berberis in the family Berberidaceae, and it is a unique plant in China. Distributed in Hunan, Yunnan, Sichuan, Xinjiang and other places in mainland China, it grows at an altitude of 600 meters to 2,800 meters. Barberry black fruit is a dominant tree species distributed in shallow mountains in arid areas, and it has important ecological functions in water conservation and soil conservation. At the same time, Berberis nigrum is a good wild plant medicine, which has been widely used and paid attention to in national medicine. The stem bark and root of Berberis nigrum contain berberine, which can detoxify and relieve fire when used as medicine. Invigorate the stomach, clear away heat and detoxify. For gastroenteriti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 张毓涛李吉玫
Owner FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
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