31results about How to "Excellent breed" patented technology

The invention discloses an inducing method of cluster buds of brasenia schreberi and belongs to the field of tissue culture of brasenia schreberi. The inducing method comprises the steps as follows: selection and pretreatment of explants, sterilization of the explants, induction of buds and multiplication culture of the buds. Components of a culture medium comprise macroelements, microelements, ferric salt, a complexing agent, an organic component, an inorganic additive, a plant growth regulator, a physiological activator, a carbon source, a coagulator and other additives. With the adoption of the culture medium and the culture method for induction of the cluster buds of the brasenia schreberi, the induction rate of the buds of the brasenia schreberi can be effectively increased, the bud differentiation rate is high, a large number of high-quality test-tube plantlets can be formed later in a short term, and seedlings are provided for culture of special aquatic vegetables in China. The inducing method can be used for breeding of the brasenia schreberi and can also be applied to rapid in-vitro propagation and germplasm preservation of the brasenia schreberi and other fields.

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

The invention discloses a method for supplementing seedlings of healthy sugarcane seedlings and prolonging the life of perennial roots. The method is to cut off the bud eyes of healthy sugarcane seedlings from the cane stem, and after being sterilized, cultivate them in a seedling tray to 4-4. 5 true leaves, and then apply the obtained seedlings to conventionally planted new plants and perennial root sugarcane fields to prolong the life of the perennial roots. With this method, when the sugarcane seeds are obtained, the remaining cane stalk is still a whole cane stalk, which can continue to be put into the sugar factory to squeeze sugar, which greatly saves the cane stalk; Germinate and grow up, and then replant the conventionally planted new plants and the place where the perennial root sugarcane is missing, the replanted sugarcane seedlings can be made to grow consistent with the previous sugarcane seedlings, and the life of the perennial roots can be extended.

The present invention relates to a kind of asexual reproduction method of red umbrella, comprising: (1) explant pretreatment; (2) cutting the explant after pretreatment into several small explants, each small explant belt There are 1-3 stem nodes, and the small explants are inoculated on the induction medium and cultivated until the clustered buds form; (3) separate the clustered buds in the induction medium, so that the clustered shoots length is controlled at 2-3cm; The clumps of shoots were inoculated into the proliferation medium and cultivated. When the new shoots grew to 2-3cm, the new shoots were separated and inserted in the proliferation medium for repeated subculture for 3-4 times to obtain a large number of clumps of shoots after propagation; (4) Split the clustered buds with a length of 3-5 cm from the base of the clustered buds after multiplication, cut them and plant them. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, so as to quickly establish a sterile propagation system for red umbrellas, provide technical support for maintaining the excellent seed properties of red umbrellas, and provide a steady stream of sterile breeding for the majority of fans. Bacteria seedlings.

The invention provides a tissue culture propagation method of Gynura formosana. The method comprises the following steps of: with the stem of Gynura formosana as an explant, carrying out sterilizing, grafting and inducing operations to form callus; then, forming an integral plant seedling through proliferation of callus, bud induction and root induction; transplanting the integral plant seedling to a culture medium so that the integral plant seedling grows up inti normal Gynura formosana plant, wherein the basic culture medium is based on MS culture medium and assisted with components such as L-proline, 6-benzyladenine, 2, 4-dichlorphenoxyacetic acid, naphthylacetic acid, cane sugar, active carbon and the like. The method provided by the invention overcomes the problem of long culturing period and low propagation coefficient of Gynura formosana by using tissue culture, and industrial quick seedling can be performed, so that the method meets demand on Gynura formosana in market.

The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culture and rapid propagation of cherry plums. The specific steps of the method are as follows: sterilize and sterilize the stem section of the axillary buds of the cherry plum as explants, first inoculate them on the bud induction medium for primary culture to obtain the axillary buds, and then inoculate the axillary buds on the proliferation medium for propagation and culture , the multi-cluster buds obtained from multiplication were cut and divided into 2-4 small parts and inoculated into GA-containing 3 Heightening culture was carried out on the heightening medium, and then the sterile seedlings with a height of more than 2 cm were excised and inoculated on the rooting medium to take root, and the remaining low and dense multi-cluster buds were subcultured on the proliferation medium and cycled Wait until the aseptic seedling base on the rooting medium grows white taproots and some fibrous roots, then harden the seedlings, transplant them into the substrate, and obtain seedlings of cherry plums. The tissue culture rapid propagation method of the present invention is easier to obtain strong seedlings than single-use multiplication culture, and the multiplication coefficient is more than 3 times higher than that of single-use increased culture.

The invention relates to a method for cultivating polyploid poplar and its application. The method comprises inserting poplar branches into a culture medium and cultivating them at 15-25°C and 50-80% relative humidity for 3-10 days. Incisions are made on the poplar branches to form callus; polyploid mutagen is used to treat the callus until the mutagenesis is sufficient, and the culture is continued after the treatment is removed. The present invention finds that the cuts on poplar cutting branches can be cultivated under suitable temperature and humidity conditions to form callus, and further treatment with polyploid mutagen can realize the cultivation of polyploid poplar, and the induction rate reaches about 42.9%. . The present invention solves the problems of poplar somatic cell chromosome doubling process or relying on embryological observation or tissue culture in the past, and has the advantages of easy operation, low cost, high induction efficiency, high preservation rate, etc. Seedling formation is an ideal way for poplar somatic chromosome doubling.

The invention relates to Chinese toon tissue culture and rapid propagation technology. The technology comprises the following steps: clipping an annual semi-lignified branch with plump axillary buds from a growing plant, clipping the branch into a stem section containing 1 to 2 axillary buds after sterilization, and rapidly inoculating the stem section to a differentiation induction culture medium for differentiation culture; placing the plant obtained through the differentiation culture into a propagation culture medium for propagation and rapid propagation; and when a certain quantity is reached through the propagation, putting the plant into a rootage culture medium for sound seedling rootage culture, and hardening the seedling after the sound seedling takes root. The method carries out the asexual tissue rapid propagation and disinfection through the stem apex and stem section of Chinese toon. The propagated filial generation maintains the good seed nature of the original variety, ensures the seed purity and has no diseases and insects. The technology overcomes the defect of low propagation speed lying in the conventional seed and cut root propagation, enables the artificial control on the culture and growth conditions, is not limited by natural conditions, and has a small amount of drawn material. The technology can realize the industrialized seedling culture and high-efficiency production.

The invention discloses a method utilizing herbicide Barban to rapidly cultivate asparagus all male plants strain, which comprises the following steps: 1) amphoteric strain is chosen from the asparagus varieties for production purpose; the amphoteric strain produces asparagus seeds by homologous strain inbred; 2) the asparagus seeds are soaked in Barban, and bud forcing treatment is carried out to the asparagus seeds; 3) the processed asparagus seeds are cultivated in a nutrition pot; flowering takes place after 30 to 35 days; each individual plant respectively hybrid with a female strain, and the seeds of each individual plant are reserved; 4) the seed reserved from each individual plant are processed again by Barban; after flowing, the ratio of male and female flowering is calculated, and super male plants are identified; 5) super male plant clones are reserved and propagated by in vitro culture method; 6) the super male plant is used as the male parent to hybrid excellent female line, and asparagus all male plants strain can be acquired. The method has the advantages that, the time of asparagus from seedling to flowering is greatly reduced; the identification process of super male plants is accelerated; the invention has an important role in the breeding of asparagus all male plants strain, and the application prospect is wide.

The invention discloses a sugarcane healthy seedling bud eye seedling and a mechanical transplanting method thereof. The method comprises the following steps of cutting the sugarcane healthy seedlingbub eye from a sugarcane stem, sterilizing, cultivating the bub eye in a seedling tray to grow to 4-5 pieces of true leaves, and then transplanting into fields by machinery. The method can obtain sugarcane seeds while the sugarcane stem is still a whole sugarcane stem, can continue to enter a sugar house for sugar extraction, and greatly save the sugarcane stem; the sugarcane healthy seedling budeye are cut and then subjected to nutrient seedling cultivation and then transplanted by the machinery, sugarcane bud rate and transplant survival rate are high, and the method can quickly get sugarcane seedlings, be suitable for large-scale planting of sugarcane, greatly reduce the cost of sugarcane cultivation and improve the planting efficiency of the sugarcane.

The present invention relates to a kind of tissue culture method of ornamental aquatic plant Antarctica, comprising the following steps: (1) pretreatment; (2) cutting the pretreated explant into 1-1.5cm long explant, each cut Each explant has at least one axillary bud; the explant after cutting is inserted into the first-generation culture medium and carried out the first-generation culture; (3) separate the first-generation adventitious buds in the first-generation medium, so that the length of the first-generation adventitious buds is controlled within 2cm; The first-generation adventitious buds after that are transferred to the propagation medium for cultivation; (4) the plants with a length greater than 3-4cm in the propagation medium are transferred to the rooting medium for cultivation; Take it out from the base, rinse it with clean water and transplant it into an ecological tank. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, and quickly establish an aseptic propagation system for Aratarctica. Seedlings.