31results about How to "Excellent breed" patented technology

The invention belongs to the technical field of microorganisms, relates to a method for preserving and separating a toadstool strain through sporocarp dried in the shade, and aims to solve the technical problems that a conventional toadstool strain preservation method is limited by season, complex to operate, easy in causing pollution and relatively high in cost. According to the technical scheme, the method for preserving and separating toadstool strain through sporocarp dried in the shade comprises the following steps: a, preserving the strain; b, separating the strain. The method provided by the invention is low in cost, simple to operate, unlimited by season, capable of effectively preserving activity of the strain, and convenient in transporting and preserving the strain.

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

The invention relates to a tissue culture method for cultivating amaryllis vittata by utilizing bulbs. The tissue culture method comprises the following steps: selecting and disinfecting explants; performing induction culture; performing subculture multiplication culture; rooting; transplanting. According to the invention, small bulbs of amaryllis vittata are taken as explants, so that the reduction of contamination rate is benefited and the induction rate is increased; cluster buds can be induced without callus stage; the cluster buds can be directly induced so as to acquire bulbs; the induction time is shortened; the buds can be added for multiplication culture; the multiplied test bulbs are rooted; one-step bulb forming of amaryllis vittata bulbs can be realized according to the invention; the time of tissue culture is saved; the operation steps are reduced; the excellent natures of original bulbs are kept; an effective method is supplied for industrial efficient cultivation of amaryllis vittata; a technical support is supplied for the industrial development of amaryllis vittata.

The invention discloses a method utilizing herbicide Barban to rapidly cultivate asparagus all male plants strain, which comprises the following steps: 1) amphoteric strain is chosen from the asparagus varieties for production purpose; the amphoteric strain produces asparagus seeds by homologous strain inbred; 2) the asparagus seeds are soaked in Barban, and bud forcing treatment is carried out to the asparagus seeds; 3) the processed asparagus seeds are cultivated in a nutrition pot; flowering takes place after 30 to 35 days; each individual plant respectively hybrid with a female strain, and the seeds of each individual plant are reserved; 4) the seed reserved from each individual plant are processed again by Barban; after flowing, the ratio of male and female flowering is calculated, and super male plants are identified; 5) super male plant clones are reserved and propagated by in vitro culture method; 6) the super male plant is used as the male parent to hybrid excellent female line, and asparagus all male plants strain can be acquired. The method has the advantages that, the time of asparagus from seedling to flowering is greatly reduced; the identification process of super male plants is accelerated; the invention has an important role in the breeding of asparagus all male plants strain, and the application prospect is wide.

The invention discloses an angelica tissue culturing and breeding method, which comprises the following steps: adopting angelica root as external growing bulk, sterilizing, grafting to induce callus, breeding, proceeding bud and root induction, forming entire plant sprout, planting the entire sprout in the substrate, adopting MS culture medium as base and 6-furfuryl adenine, 6-benzyl adenine, 2, 4-dichlorophenoxyacetic acid, fruitone, sucrose and active carbon as auxiliary compositions.

The invention provides a method for quickly breeding plumeria rubra by tissue culture. The method comprises the following steps of carrying out tissue culture and rapid propagation with MS (Murashige and Skoog) as a minimal medium and plumeria rubra leaves as explants under different hormone components and concentration levels; carrying out sterilization treatment and inoculation induction to form a callus tissue, and then carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; and finally carrying out acclimatization and transplantation, wherein the minimal medium is based on the MS culture medium, and supplemented with components including 6-benzyl aminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the invention, tissue culture is applied to overcome the problem that the plumeria rubra is too long in seedling-raising period and low in propagation coefficient, so that factory-like quick seedling raising can be carried out for meeting the demands of the market on the plumeria rubra.

The invention discloses a method for prolonging the life of ratoons by filling healthy sugarcane seedling bud recesses with seeds. The method comprises the following steps: cutting the sugarcane healthy seed bud recesses from a sugarcane stem, disinfecting and sterilizing the sugarcane healthy seed bud recesses, culturing the sugarcane healthy seed bud recesses in a seedling culture tray till 4 to5 main leaves grow, and then applying the obtained seedlings to fill newly planted and ratoon sugarcanes in conventional planting to prolong the life of the ratoons. According to the method, sugarcane seeds are obtained, while the remaining sugarcane stem is still an overall sugarcane stem and can be continuously delivered into a sugar refinery for sugar refining, so that the sugarcane stems aregreatly saved; after the healthy sugarcane seedling bud recesses are cut off, the bud recesses are germinated and grow rapidly through nutritional seedling culture at first, and then seed-lacking places of the newly planted and ratoon sugarcanes in conventional planting are filled with the seeds, so that the seed-filled sugarcane seedlings and the previous sugarcane seedlings grow consistently, and the life of the ratoons is prolonged.

The invention provides a tissue culture propagation method of Gynura formosana. The method comprises the following steps of: with the stem of Gynura formosana as an explant, carrying out sterilizing, grafting and inducing operations to form callus; then, forming an integral plant seedling through proliferation of callus, bud induction and root induction; transplanting the integral plant seedling to a culture medium so that the integral plant seedling grows up inti normal Gynura formosana plant, wherein the basic culture medium is based on MS culture medium and assisted with components such as L-proline, 6-benzyladenine, 2, 4-dichlorphenoxyacetic acid, naphthylacetic acid, cane sugar, active carbon and the like. The method provided by the invention overcomes the problem of long culturing period and low propagation coefficient of Gynura formosana by using tissue culture, and industrial quick seedling can be performed, so that the method meets demand on Gynura formosana in market.

The invention discloses a phoebe bournei grafting and seedling raising method. Strong one-year phoebe bournei seedlings with the height of 40-60 cm and the ground diameter of 5-7 mm are selected as rootstock, in-season sprouting branches are selected from good provenance select trees with the height 15% larger than average height and the diameter at breast height 50% larger than average diameter at breast height or more to serve as scions, after grafting, the rootstock and the scions are prevented from being bruised or tilted and broken, sprouts are removed, bound mulching films are cut open in time, fertilizer and water management, weeding and disease and pest prevention are conducted well in time during the period, and good strong phoebe bournei grafting seedlings are obtained. The seedling raising method is easy and quick to operate, the grafting survival rate is high, new seed collecting mother plant dwarf can be promoted, the flowering and fruiting stages are earlier, safety of manual seed and cutting collection is improved, production cost is reduced, fecundity of good phoebe bournei seedlings is improved, good seed supply capacity is improved, and the requirements of development of the phoebe bournei industry are met.

The invention discloses a quick red bean local variety purifying and rejuvenating method and relates to the technical field of crop variety resource protection and utilization. The method comprises the steps: performing large group selection to select single plants and performing plant laboratory test and forming plant rows; identifying and comparing multiple plant rows and forming plant lines through the plant laboratory test; comparing multiple plant lines to predict yield and selecting high-yield plant systems through the plant laboratory test; performing mixed line breeding and producing qualified seeds. The method disclosed by the invention widens selected groups, can perform plant laboratory test according to red bean representations, ensures population genetic stability, reduces gene loss generation, quickly increases population quantity and increases output quantity of breeder seeds; seed propagation generations are reduced, a mixing opportunity is reduced, and a natural selection effect is reduced; meanwhile, a red bean local variety purifying and rejuvenating process is quickened, excellent variety characteristics are kept, germplasm resources with local characteristics are effectively protected, and the breeder seeds can be directly applied to large field production or the breeder seeds can be utilized to directly produce seeds.

The invention relates to a quality walnut tissue culture quick propagation method which comprises, appending right amount of iron ions and zinc ions into chestnut culture medium, adjusting nutrient content of culture medium, screening the most suitable culture medium for chestnut growth, selecting right explant for tissue culture at right stage, readjusting the culture medium formulation so as to grow shoot and root for the chestnut, transplanting it into warm house and planting into big nourishing cup equipped with nourishment base materials, finally planting and transplanting the nursery stock in the nourishment cup.

The invention relates to a tissue culture method of ornamental-aquatic-plant Hydrotriche hottoniflora. The method includes the steps of firstly, preprocessing; secondly, cutting preprocessed explantsinto explants 1-1.5cm in length, wherein the each cut explant at least comprises one axillary bud; inoculating the cut explants into a primary culture media to perform primary culture; thirdly, separating primary adventitious buds in the primary culture media to control the length of the primary adventitious buds to be within 2cm; transferring the separated primary adventitious buds into proliferation culture media to perform culture; fourthly, transferring plants with the length being larger than 3-4cm in the proliferation culture media into rooting culture media to perform culture; fifthly,taking the Hydrotriche hottoniflora plants with complete root systems out of the rooting culture media, washing with clean water, and transplanting into an ecological tank. The method has the advantages that the method is unaffected by seasons, temperature and regions and can acquire a large number of new plants, the sterile reproduction system of Hydrotriche hottoniflora can be built fast, technical support is provided for the keeping of the excellent variety characters of the Hydrotriche hottoniflora, and sterile seedlings can be constantly provided for vast Hydrotriche hottoniflora lovers.

The invention discloses an inducing method of cluster buds of brasenia schreberi and belongs to the field of tissue culture of brasenia schreberi. The inducing method comprises the steps as follows: selection and pretreatment of explants, sterilization of the explants, induction of buds and multiplication culture of the buds. Components of a culture medium comprise macroelements, microelements, ferric salt, a complexing agent, an organic component, an inorganic additive, a plant growth regulator, a physiological activator, a carbon source, a coagulator and other additives. With the adoption of the culture medium and the culture method for induction of the cluster buds of the brasenia schreberi, the induction rate of the buds of the brasenia schreberi can be effectively increased, the bud differentiation rate is high, a large number of high-quality test-tube plantlets can be formed later in a short term, and seedlings are provided for culture of special aquatic vegetables in China. The inducing method can be used for breeding of the brasenia schreberi and can also be applied to rapid in-vitro propagation and germplasm preservation of the brasenia schreberi and other fields.

The invention relates to a method for cultivating polyploid poplar and application thereof. The method comprises the steps that poplar branches are inserted into a culture medium and cultured for 3-10days at the temperature of 15-25 DEG C and the relative humidity of 50%-80%, and calluses are formed by notches in the poplar branches; and the calluses are treated by using a polyploid mutagenic agent until the calluses are fully mutated, treatment is ended, and culturing is continuously carried out. It is found that the notches in the poplar cutting branches can form the calluses after culturing is carried out under proper temperature and humidity conditions, polyploid poplar culture can be achieved by further applying a polyploid mutagenic agent for treatment, and the induction rate reaches about 42.9%. The method solves the technical problems of a conventional populus somatic chromosome doubling process or depending on embryology observation or depending on tissue culture and the like, has the advantages of easiness in operation, low cost, high induction efficiency, high preservation rate and the like, can ensure that induced polyploid seedlings grow up in the same year, and is anideal way for doubling populus somatic chromosomes.

The invention discloses an angelica tissue culturing and breeding method, which comprises the following steps: adopting angelica root as external growing bulk, sterilizing, grafting to induce callus, breeding, proceeding bud and root induction, forming entire plant sprout, planting the entire sprout in the substrate, adopting MS culture medium as base and 6-furfuryl adenine, 6-benzyl adenine, 2, 4-dichlorophenoxyacetic acid, fruitone, sucrose and active carbon as auxiliary compositions.

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

The invention relates to an asexual propagation method of proserpinaca palustris. The method includes: (1), pre-treating an explant; (2), cutting the explant after being pre-treated into a plurality of small explants, and inoculating the small explants onto an induced culture medium for culture until clustered shoots are formed, wherein each small explant has 1-3 stem nodes; (3), separating the clustered shoots in the induced culture medium to control length of the clustered shoots to be 2-3cm; inoculating the clustered shoots after being separated into a proliferation culture medium for culture, separating new shoots when growing to 2-3cm, inserting the new shoots into the proliferation culture medium, and repeating subculture for 3-4 times to obtain clustered shoots after large-scale propagation; (4), splitting down the clustered shoots of 3-5cm in length in the clustered shoots after propagation from bases to shoot tubers, cutting, and planting. By the method, a lot of new plants can be obtained without being subject to season, temperature and regional impact, so that a proserpinaca palustris sterile propagation system can be established quickly, technical support can be provided for maintaining excellent seed characteristics of proserpinaca palustris, and ceaseless sterile seedlings are provided for the majority of fans.

The invention relates to a method for tissue culture of aseptic seedlings of Glycyrrhizae, comprising the following steps: (1) preparing primary culture medium and proliferation medium, and sterilizing them for use; (2) cleaning and disinfecting explants; (3) Absorb the surface moisture of the sterilized explants with sterilized paper, transfer them into the primary culture medium, then pour 30-60ml of cooled sterile water into the tissue culture bottle, and sink the explants in sterile water. In water, place it in an incubator to induce culture for 4 weeks; (4) separate the explants and the sprouts produced in the primary culture medium, transfer them to the proliferation medium together, pour 30-60ml of cooled sterile water, and submerge New explants and new shoots were proliferated and cultured for 4 weeks; (5) the plants with complete root systems were taken out, rinsed with clean water, and then transplanted into simulated natural water bodies for rooting culture. The method can obtain a large number of new plants without being affected by seasons, temperatures and regions, the rooting rate of the new plants reaches 100%, the survival rate is high, and the aseptic propagation system of the algae can be quickly established.

The invention discloses a method for large-scale breeding of virus-free lily balls. The steps of the method are as follows: a. Ovary selection and marking: Lily plants are selected in the field, and the ovary is taken out and marked; b. Virus detection of the ovary: Cut out the ovary with a length of 4-7mm and use the multiple RT-PCR method for detection, and put the non-toxic ovary into the refrigerator for temporary storage; c, ovary pretreatment: rinse with running water, disinfect and sterilize, and then rinse with sterile water , the ovary is vertically cut into two parts and placed in the pretreatment medium for cultivation; d, ovary induced callus culture: the pre-cultured ovary is cut into 2mm cut pieces and placed in the induction medium for cultivation; e , Induction into balls: Transfer the callus to the medium for inducing buds, culture in the dark for 1-2 months and then subculture and multiply once. After 3-4 months, you can get lilies with a diameter of 0.3-2cm The small bulbs are the non-toxic bulbs of lilies. The process of the method of the invention is simple to operate and easy to be implemented on a large scale.

The invention belongs to the technical field of agricultural biology and particularly relates to a tissue culture rapid propagation method of prunus cerasifera. The method comprises the following steps: prunus cerasifera with axillary bud stems is sterilized and disinfected to serve as an explant, a bud induction medium is firstly inoculated with the explant for primary culture to obtain axillarybud seedlings, then, a proliferation medium is inoculated with the axillary bud seedlings for proliferation culture, multi-cluster buds obtained by proliferation are cut an divided into 2-4 parts, a height increase medium containing GA3 is inoculated with the multi-cluster buds for height increase culture, single aseptic seedling at a height of 2 cm or above is cut off, a rooting medium is inoculated for rooting, the rest low and dense multi-cluster buds are subcultured again to the proliferation medium in a circulating manner, seedling exercise is performed after aseptic seedlings on the rooting medium come up white taproots and multiple fibrous roots, the seedlings are transplanted to a medium, and seedlings of the prunus cerasifera are obtained. The tissue culture rapid propagation method can obtain strong seedlings more easily than single proliferation culture, and the proliferation coefficient is 3 times higher than that with single height increase culture.

The invention discloses a tissue culture method of wild sambucus williamsii and belongs to the field of plant tissue culture. An asexual reproduction method of the sambucus williamsii is in a blank stage. The tissue culture method of wild sambucus williamsii comprises the following steps: selecting a stem segment with a lateral bud as an explant; conducting disinfection and inoculating the disinfected explant into a primary culture medium for culturing to form the explant with a bud; inoculating the obtained explant with a bud into a proliferation culture medium for culturing; cutting off an adventitious bud and inoculating the adventitious bud into an MS culture medium for culturing; and and inoculating the cultured adventitious bud into a rooting culture medium for culturing. The method has advantages of having a high propagation coefficient, a short propagation period, and low cost and keeping good varieties.

The invention discloses a method for supplementing seedlings of healthy sugarcane seedlings and prolonging the life of perennial roots. The method is to cut off the bud eyes of healthy sugarcane seedlings from the cane stem, and after being sterilized, cultivate them in a seedling tray to 4-4. 5 true leaves, and then apply the obtained seedlings to conventionally planted new plants and perennial root sugarcane fields to prolong the life of the perennial roots. With this method, when the sugarcane seeds are obtained, the remaining cane stalk is still a whole cane stalk, which can continue to be put into the sugar factory to squeeze sugar, which greatly saves the cane stalk; Germinate and grow up, and then replant the conventionally planted new plants and the place where the perennial root sugarcane is missing, the replanted sugarcane seedlings can be made to grow consistent with the previous sugarcane seedlings, and the life of the perennial roots can be extended.

The present invention relates to a kind of asexual reproduction method of red umbrella, comprising: (1) explant pretreatment; (2) cutting the explant after pretreatment into several small explants, each small explant belt There are 1-3 stem nodes, and the small explants are inoculated on the induction medium and cultivated until the clustered buds form; (3) separate the clustered buds in the induction medium, so that the clustered shoots length is controlled at 2-3cm; The clumps of shoots were inoculated into the proliferation medium and cultivated. When the new shoots grew to 2-3cm, the new shoots were separated and inserted in the proliferation medium for repeated subculture for 3-4 times to obtain a large number of clumps of shoots after propagation; (4) Split the clustered buds with a length of 3-5 cm from the base of the clustered buds after multiplication, cut them and plant them. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, so as to quickly establish a sterile propagation system for red umbrellas, provide technical support for maintaining the excellent seed properties of red umbrellas, and provide a steady stream of sterile breeding for the majority of fans. Bacteria seedlings.

The invention provides a tissue culture propagation method of Gynura formosana. The method comprises the following steps of: with the stem of Gynura formosana as an explant, carrying out sterilizing, grafting and inducing operations to form callus; then, forming an integral plant seedling through proliferation of callus, bud induction and root induction; transplanting the integral plant seedling to a culture medium so that the integral plant seedling grows up inti normal Gynura formosana plant, wherein the basic culture medium is based on MS culture medium and assisted with components such as L-proline, 6-benzyladenine, 2, 4-dichlorphenoxyacetic acid, naphthylacetic acid, cane sugar, active carbon and the like. The method provided by the invention overcomes the problem of long culturing period and low propagation coefficient of Gynura formosana by using tissue culture, and industrial quick seedling can be performed, so that the method meets demand on Gynura formosana in market.

The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culture and rapid propagation of cherry plums. The specific steps of the method are as follows: sterilize and sterilize the stem section of the axillary buds of the cherry plum as explants, first inoculate them on the bud induction medium for primary culture to obtain the axillary buds, and then inoculate the axillary buds on the proliferation medium for propagation and culture , the multi-cluster buds obtained from multiplication were cut and divided into 2-4 small parts and inoculated into GA-containing 3 Heightening culture was carried out on the heightening medium, and then the sterile seedlings with a height of more than 2 cm were excised and inoculated on the rooting medium to take root, and the remaining low and dense multi-cluster buds were subcultured on the proliferation medium and cycled Wait until the aseptic seedling base on the rooting medium grows white taproots and some fibrous roots, then harden the seedlings, transplant them into the substrate, and obtain seedlings of cherry plums. The tissue culture rapid propagation method of the present invention is easier to obtain strong seedlings than single-use multiplication culture, and the multiplication coefficient is more than 3 times higher than that of single-use increased culture.

The invention relates to a method for cultivating polyploid poplar and its application. The method comprises inserting poplar branches into a culture medium and cultivating them at 15-25°C and 50-80% relative humidity for 3-10 days. Incisions are made on the poplar branches to form callus; polyploid mutagen is used to treat the callus until the mutagenesis is sufficient, and the culture is continued after the treatment is removed. The present invention finds that the cuts on poplar cutting branches can be cultivated under suitable temperature and humidity conditions to form callus, and further treatment with polyploid mutagen can realize the cultivation of polyploid poplar, and the induction rate reaches about 42.9%. . The present invention solves the problems of poplar somatic cell chromosome doubling process or relying on embryological observation or tissue culture in the past, and has the advantages of easy operation, low cost, high induction efficiency, high preservation rate, etc. Seedling formation is an ideal way for poplar somatic chromosome doubling.

The invention relates to Chinese toon tissue culture and rapid propagation technology. The technology comprises the following steps: clipping an annual semi-lignified branch with plump axillary buds from a growing plant, clipping the branch into a stem section containing 1 to 2 axillary buds after sterilization, and rapidly inoculating the stem section to a differentiation induction culture medium for differentiation culture; placing the plant obtained through the differentiation culture into a propagation culture medium for propagation and rapid propagation; and when a certain quantity is reached through the propagation, putting the plant into a rootage culture medium for sound seedling rootage culture, and hardening the seedling after the sound seedling takes root. The method carries out the asexual tissue rapid propagation and disinfection through the stem apex and stem section of Chinese toon. The propagated filial generation maintains the good seed nature of the original variety, ensures the seed purity and has no diseases and insects. The technology overcomes the defect of low propagation speed lying in the conventional seed and cut root propagation, enables the artificial control on the culture and growth conditions, is not limited by natural conditions, and has a small amount of drawn material. The technology can realize the industrialized seedling culture and high-efficiency production.

The invention discloses a method utilizing herbicide Barban to rapidly cultivate asparagus all male plants strain, which comprises the following steps: 1) amphoteric strain is chosen from the asparagus varieties for production purpose; the amphoteric strain produces asparagus seeds by homologous strain inbred; 2) the asparagus seeds are soaked in Barban, and bud forcing treatment is carried out to the asparagus seeds; 3) the processed asparagus seeds are cultivated in a nutrition pot; flowering takes place after 30 to 35 days; each individual plant respectively hybrid with a female strain, and the seeds of each individual plant are reserved; 4) the seed reserved from each individual plant are processed again by Barban; after flowing, the ratio of male and female flowering is calculated, and super male plants are identified; 5) super male plant clones are reserved and propagated by in vitro culture method; 6) the super male plant is used as the male parent to hybrid excellent female line, and asparagus all male plants strain can be acquired. The method has the advantages that, the time of asparagus from seedling to flowering is greatly reduced; the identification process of super male plants is accelerated; the invention has an important role in the breeding of asparagus all male plants strain, and the application prospect is wide.

The present invention relates to a kind of tissue culture method of ornamental aquatic plant Antarctica, comprising the following steps: (1) pretreatment; (2) cutting the pretreated explant into 1-1.5cm long explant, each cut Each explant has at least one axillary bud; the explant after cutting is inserted into the first-generation culture medium and carried out the first-generation culture; (3) separate the first-generation adventitious buds in the first-generation medium, so that the length of the first-generation adventitious buds is controlled within 2cm; The first-generation adventitious buds after that are transferred to the propagation medium for cultivation; (4) the plants with a length greater than 3-4cm in the propagation medium are transferred to the rooting medium for cultivation; Take it out from the base, rinse it with clean water and transplant it into an ecological tank. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, and quickly establish an aseptic propagation system for Aratarctica. Seedlings.