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Tissue culture rapid propagation method of prunus cerasifera

A technology of tissue culture and rapid propagation of cherries, applied in the field of agricultural biology, can solve the problems of low rooting rate of cherry plums and the inability to ensure the uniformity of seedlings, etc., and achieve the effects of easy to obtain strong seedlings, increase the effect of growth and multiplication, and increase the multiplication coefficient

Active Publication Date: 2020-08-04
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, seed reproduction is sexual reproduction, and traits will be separated, so the uniformity of seedlings cannot be guaranteed. Cutting propagation is asexual reproduction, which can ensure the uniformity of seedlings, but the rooting rate of cherry plums is low.

Method used

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  • Tissue culture rapid propagation method of prunus cerasifera
  • Tissue culture rapid propagation method of prunus cerasifera
  • Tissue culture rapid propagation method of prunus cerasifera

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The establishment of embodiment 1 cherry plum tissue culture rapid propagation method

[0040] The present embodiment provides the rapid propagation method of cherry plum tissue culture, and its flow process is as follows figure 1 shown.

[0041] 1. Explant collection

[0042]Using the current-year-old stems of cherry plum seedlings as explants, the robust, semi-lignified middle stems of the current-year shoots were collected in June, and the leaves at the stem nodes were removed and rinsed with tap water. The upper part of the axillary bud is about 0.2 cm and the lower part is about 1.5 cm, respectively, and the stem section with the axillary bud is obtained, which is placed in a petri dish for later use;

[0043] 2. Disinfection of explants

[0044] Soak the stem section with axillary buds obtained on the ultra-clean workbench with 75% alcohol for 15 seconds, then soak for 15 minutes with 3% sodium hypochlorite, rinse 6 times with sterile water, and dry the moistur...

Embodiment 2

[0076] Example 2 Rapid Propagation of Cherry Plum Varieties "Senguo Honglu" and "Senguo Jiaren"

[0077] Utilize the Tissue Culture Rapid Propagation Method of Cherry Plum established in Example 1 to carry out rapid propagation respectively to Cherry Plum varieties "Senguo Honglu" and "Senguo Beauty", the specific methods are as follows:

[0078] The current year's stems of "Senguo Honglu" and "Senguo Jiaren" were taken as explants, and the robust, semi-lignified stems in the middle of the new shoots were collected, and the leaves at the stem nodes were removed and rinsed with tap water. Clean, cut off about 0.2 cm above and 1.5 cm below the axillary buds of each stem node to obtain stem segments with axillary buds, and put them in a petri dish for later use;

[0079] Step 1, soak the obtained stem section with axillary buds with 75% alcohol for 15 seconds, then soak it with 3% sodium hypochlorite for 15 minutes, rinse it with sterile water for 6 times, dry the surface moistur...

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Abstract

The invention belongs to the technical field of agricultural biology and particularly relates to a tissue culture rapid propagation method of prunus cerasifera. The method comprises the following steps: prunus cerasifera with axillary bud stems is sterilized and disinfected to serve as an explant, a bud induction medium is firstly inoculated with the explant for primary culture to obtain axillarybud seedlings, then, a proliferation medium is inoculated with the axillary bud seedlings for proliferation culture, multi-cluster buds obtained by proliferation are cut an divided into 2-4 parts, a height increase medium containing GA3 is inoculated with the multi-cluster buds for height increase culture, single aseptic seedling at a height of 2 cm or above is cut off, a rooting medium is inoculated for rooting, the rest low and dense multi-cluster buds are subcultured again to the proliferation medium in a circulating manner, seedling exercise is performed after aseptic seedlings on the rooting medium come up white taproots and multiple fibrous roots, the seedlings are transplanted to a medium, and seedlings of the prunus cerasifera are obtained. The tissue culture rapid propagation method can obtain strong seedlings more easily than single proliferation culture, and the proliferation coefficient is 3 times higher than that with single height increase culture.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culture and rapid propagation of cherry plums. Background technique [0002] Cherry plum (scientific name: Prunus cerasifera Ehrhart) is a plant of the genus Prunus in the Rosaceae family. It is a precious wild fruit tree resource in Ili wild fruit forest in Xinjiang. extremely important conservation value. However, at present, wild cherry plum resources continue to decrease and are on the verge of extinction and urgently need to be protected. Cherry plums have strong stress resistance and can be used as rootstocks for cultivated fruit trees such as peaches, plums, and apricots. Through cultivation and utilization, the protection and utilization of cherry plum germplasm resources can be realized. Cherry plums can be propagated by seeds, cuttings, etc. But seed propagation is sexual reproduction, character separation can occur, and the u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 冀晓昊王海波刘凤之王孝娣王莹莹
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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