31 results about "Prunus pseudocerasus" patented technology

The invention discloses a method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus. The method is characterized in that the tissue culture seedlings of Gisela 5 and 6 in the root induction stage, which are produced in the laboratory, are adopted; on the 14th day of root induction, the shooting-up seedlings are selected and are further cultured into strong seedlings; the seedlings can be used after the seedlings become slightly strong half a month later; the matrix used for cuttage is formed by uniformly mixing pearlite, vermiculite, turfy soil and river sands in a ratio of 2:2:1:1; in the period, the temperature and the humidity in the greenhouse are respectively ensured to be 26-28 DEG C and 50-60%; the bactericide is sprayed every other day; a kung fu insecticide is sprayed 5-7 days after cuttage; the spraying times of the insecticide depends and is not more than 5; the insecticide is sprayed every 2-3 days; whether the seedlings root can be observed onthe 10th-14th day; and the plantlets can be transplanted to plugs to be acclimatized 20 days later. The experimental results show that by adopting the method, each plant can have 3 roots, the cycle is short and the effect is good; and the technology can be used for scale production of Gisela virus-free stocks.

The invention relates to a compound oil health food which is characterized by comprising the following raw materials in parts by weight: 20-50 parts of seahero colza oil, 10-30 parts of prunus pseudocerasus seed oil, 10-30 parts of grape seed oil, 10-20 parts of apricot kernel oil, 5-10 parts of tomato seed oil and 0.01-0.02 part of antioxidant. By fully utilizing the health effects of the raw materials and carrying out scientific formulation, the compound oil health food has balanced nutrition components and convenient taking, can fully utilize the compounded effective nutrition components thereof and is beneficial to body health after being taken for a long term.

The invention provides primers for detecting SSR (simple sequence repeat) marks of prunus pseudocerasus. The group of primers comprises nine primer pairs which can be used simultaneously in the detection process, wherein the sequences of the nine primer pairs are shown in SEQ ID NO:1 to SEQ ID NO:18. The nine SSR marks screened out have polymorphism in species or varieties of prunus pseudocerasus, genetic diversity analysis performed in 22 species or varieties of prunus pseudocerasus basically coincides with classification common sense of cerasus plants, the SSR marks are new marks existing stably, and the nine marks provided in the invention can be directly applied to more prunus pseudocerasus or even transferred to prunus avium for genetic diversity analysis of germplasm resources, construction of genetic maps and molecular mark assisted breeding.

The invention relates to a health prunus cerasifera wine and a preparation method thereof and belongs to the technical field of preparation of health foods. The health prunus cerasifera wine is prepared by soaking prunus cerasifera in edible alcohol. The health prunus cerasifera wine has dark ruby red color and unique flavor and is sweet, tart and palatable, rich in prunus cerasifera fresh fruit flavor and high in quality and sensory evaluations. Compared with the commercially available health wine, the prunus cerasifera wine which takes prunus cerasifera as a main additive has health care function and no side effects. The health prunus cerasifera wine added with a mulberry extract, a red date extract and a wolfberry extract can stimulate multiple genes in the human body to inhibit the growth of cancer cells, can prevent the formation of embolism in blood cells and has the effects of well promoting immunity, reducing blood sugar, resisting oxidation and aging, delaying senescence, promoting apoptosis of tumor cells, resisting radiation damage, reducing blood fat and the like and meanwhile, the contents of total flavonoid, anthocyanin and polysaccharide and the antioxidant activity are significantly increased.

The invention discloses a method and a special primer for simultaneously detecting whether a cherry to be infected by an apple chlorotic leaf spot virus and a prunus necrotic ringspot virus. The primer used for detecting plant viruses comprises a primer pair A and a primer pair B, wherein the primer pair A is used for detecting the apple chlorotic leaf spot virus; a primer sequence is shown as SEQ ID No.1 (sequence identification number 1); the other primer sequence is shown as SEQ ID No.2; the primer pair B is used for detecting the prunus necrotic ringspot virus; a primer sequence is shown as SEQ ID No.3; the other primer sequence is shown as SEQ ID No.4. A multi-RT-PCR (reverse transcription-polymerase chain reaction) established in the primer can simultaneously detect ACLSV (apple chlorotic leaf spot virus) and PNRSV (prunus necrotic ringspot virus) compounded infection of the cherry in production, and saves time compared with a single RT-PCR; the operation is simpler; in addition, consumed drugs in a test are reduced; and economy is facilitated. When a total amount of RNA (ribonucleic acid) of an infected plant in a reverse transcription system ranges from 10 micrograms to 4 micrograms, the two viruses can still be detected simultaneously after PCR (polymerase chain reaction) amplification is performed.

The present invention discloses special micro-organism-inhibiting and freshness-retaining paper for cerasus pseudocerasus fruits and a freshness-retaining method thereof. The freshness-retaining paperis prepared from 350x190 mm double-layer non-woven fabric, 1% of a 1-methylcyclopropene adsorbent and 0.1% of garlic essential oil and prepared by two internal and external successive printing on thedouble-layer nonwoven fabric. The special micro-organism-inhibiting and freshness-retaining method for the cerasus pseudocerasus fruits comprises the following steps: 0.25 g of 1-methylcyclopropene medicinal powder is uniformly placed in middle of the 350x190 mm double-layer non-woven fabric; the double-layer non-woven fabric loaded with the 1-methylcyclopropene medicinal powder is subjected to edge sealing using a non-woven fabric sewing machine; and 0.1% of garlic essential oil is printed on center of the non-woven fabric in a cross-type, and the non-woven fabric printed with the garlic essential oil is quickly dried and bagged for use. Advantages are that: 1 sheet of the freshness-retaining paper can conduct seal freshness-retaining of 3-5 kilograms of the cerasus pseudocerasus fruits;and the special micro-organism-inhibiting and freshness-retaining paper is simple in formula, safe, easy to operate, low in cost, good in freshness-retaining effects, high in stability, high in broad-spectrum applicability, and easy for wide promotion and application.

The invention discloses a tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings. The tissue culture method comprises hybridization seed production and seed collection. A collected seed comprises the following steps of in sequence: S1: carrying out seed hull-breaking processing and seed embryo dipping; S2: carrying out seed embryo disinfection and inner seed coat removal; S3: carrying out inoculation and culture; S4: checking an embryo pollution situation and secondary disinfection; S5: carrying out browning processing and timely transferring; andS6: carrying out acclimatization and transplantation. The tissue culture method only uses one MS solid culture medium which contains an exogenous growth promoting additive, time for taking roots fromgermination is short, the tissue culture method is simple and convenient to operate, hybridization F1-generation seedling growth time is greatly shortened, a whole seed breeding process is shortened,and Pruns avium L breeding efficiency is improved.

The invention provides a fructus hippophae fermentation liquid. The fructus hippophae fermentation liquid is characterized by comprising the following components in parts by weight: 20-50 parts of fructus hippophae, 15-30 parts of fructus citri limoniae, 30-60 parts of fructus pruni pseudocerasi, 20-45 parts of fructus ananadis comosi, 15-40 parts of saccharum sinensis roxb., 20-45 parts of oligofructose and 16-56 parts of strains. Therefore, the prepared fructus hippophae fermentation liquid is relatively good in taste, can be suitable for all people to eat, can achieve the effects of adjusting intestinal microflora and fully releasing beneficial ingredients of various components and has a very positive market prospect.

The invention discloses a composite plant extract cosmetic preservative. The composite plant extract cosmetic preservative is prepared by mixing the following raw materials: rosa centifolia flower extract, fragaria vesca leaf extract and prunus pseudocerasus leaf extract, wherein the mass ratio of the rosa centifolia flower extract to the fragaria vesca leaf extract to the prunus pseudocerasus leaf extract is (1 to 3) to (1 to 5) to (1 to 10). Compared with the prior art, the composite plant extract cosmetic preservative disclosed by the invention has the beneficial effects that natural plantsourced raw material components are used for replacing a traditional preservative; excellent anti-corrosion and antibacterial performance is provided for a cosmetic product; meanwhile, the possibilityof stimulating skin by the traditional preservative is avoided, so that the rate of allergy of using cosmetics is greatly reduced.

The invention relates to the technical field of plant virus detection, and in particular relates to a method for detecting prunus necrotic ring-spot virus of sweet cherries. The method comprises the following steps of: (1), designing and synthesizing a DNA fragment and a probe; (2), preparing nanogold; (3), preparing a nanogold-probe 1 compound; (4), assembling a test strip; (5), preparing a sample; (6), performing exonuclease III signal amplification; and (7), detecting the test strip. According to the method in the invention, the test strip is prepared by adoption of a nucleic acid detectionprobe; an antibody is unnecessary to prepare; the preparation process is simple; the cost is low; the probe is designed for a highly specific conserved region of the prunus necrotic ring-spot virus of the sweet cherries; the detection effect is good; the specificity is high; false positive is difficultly generated; signal amplification is carried out by utilization of cyclic enzyme digestion of exonuclease III; the detection sensitivity is increased; furthermore, a professional staff and a special instrument are unnecessary; carrying and detection are convenient; a result can be measured by naked eyes; the colour development time is short; and the colour development time is only 10 min.

The invention discloses compound botanical essence oil for fading red, white and black stretch marks. The compound botanica essence oil comprises 10-90 parts of rosa canina fruit oil; 8-50 parts of prunus amygdalus dulcis oil; 0.1-35 parts of calophyllum inophyllum kernel oil; 1-5 parts of cherry seed oil; 0.1-2 parts of sea buckthorn fruit oil; and 0.1-1 part of vitamin E. According to the compound botanical essence oil for fading red, white and black stretch marks, plant extracts such as rosa canina fruit oil, prunus amygdalus dulcis oil, calophyllum inophyllum kernel oil, cherry seed oil and sea buckthorn fruit oil are selected in combination with characteristics of vitamin E in scientific ratio to achieve balance among efficacy, skin feeling and smell by mutual complementation. Therefore, the compound botanical essence oil has better effects of moistening skin and fading growth lines and scars. The invention further discloses a preparation method and an application of the compound botanical essence oil for fading red, white and black stretch marks.

The use of an extract of at least a part of rocket plants, preferably chosen from the group of rocket plants of the Eruca (Eruca sativa; Eruca vesicaria, etc.), Diplotaxis (Diplotaxis erucoides, Diplotaxis tenuifolia, Diplotaxis muralis, etc.), Bunias (Bunias erucago, Bunias orientalis, etc.), Erucastrum (Erucastrum nasturtiifolium, Erucastrum incanum, etc.) and Cakile genuses, stimulates the defences of plants or trees and reduces the effects of bacteria and fungi on plants and trees.In particular, the effects:of Xylella fastidiosa bacteria on myrtle-leaf milkwort, grape vines, olive trees, citrus trees, oleander, almond trees, coffee trees, peach trees and stone fruit trees, oak trees, lavender, rosemary, or broom,of Xanthomonas arboricola pv. Pruni bacteria on Prunus spp., and preferably the following group of fruit / nut trees: apricot trees, almond trees, cherry trees, peach trees, plum trees, P. salicina, cherry laurel and other exotic or omamental Prunus species, including P. davidiana and P. laurocerasus,of Pear Decline Phytoplasma bacteria or Candidatus Phytoplasma pyri on pear trees, andof Candidatus Phytoplasma solani bacteria on grape vines, lavender, potato plants, tomato plants, aubergine plants, pepper plants and tobacco plants.

The invention belongs to the technical field of agricultural biology and particularly relates to a tissue culture rapid propagation method of prunus cerasifera. The method comprises the following steps: prunus cerasifera with axillary bud stems is sterilized and disinfected to serve as an explant, a bud induction medium is firstly inoculated with the explant for primary culture to obtain axillarybud seedlings, then, a proliferation medium is inoculated with the axillary bud seedlings for proliferation culture, multi-cluster buds obtained by proliferation are cut an divided into 2-4 parts, a height increase medium containing GA3 is inoculated with the multi-cluster buds for height increase culture, single aseptic seedling at a height of 2 cm or above is cut off, a rooting medium is inoculated for rooting, the rest low and dense multi-cluster buds are subcultured again to the proliferation medium in a circulating manner, seedling exercise is performed after aseptic seedlings on the rooting medium come up white taproots and multiple fibrous roots, the seedlings are transplanted to a medium, and seedlings of the prunus cerasifera are obtained. The tissue culture rapid propagation method can obtain strong seedlings more easily than single proliferation culture, and the proliferation coefficient is 3 times higher than that with single height increase culture.

The invention discloses fruit and vinasse porridge which comprises the following raw material components in parts by weight: 40-60 parts of black rice, 10-12 parts of milk, 60-80 parts of sago, 10-12 parts of rock candy, 20-40 parts of nectarine, 1-3 parts of flos rosae rugosae, 10-12 parts of apples, 2-4 parts of buddleja officinalis, 5-7 parts of litchi, 3-5 parts of lilium brownii, 10-12 parts of prunus pseudocerasus, 5-7 parts of red distillers' grains, 2-4 parts of lonicera japonica, 4-6 parts of bellis perennis and 1000-1200 parts of water; the fruit and vinasse porridge is prepared through cleaning, boiling, mixing, stewing and packaging. The fruit and vinasse porridge is fresh, fragrant and smooth in taste, integrates faint scents of cereals and fruits, is peculiar in flavor, full of nutriments, easy to absorb by human bodies, beneficial for human health, and at the same time has a fresh and mellow taste, thereby being particularly suitable for people whose appetite is affected by dry heat in summer and autumn.

The invention discloses an oral prescription for treatment of urethritis. The oral prescription is prepared from the following main raw materials by weight: 11-18 parts of lophatherum gracile; 9-14 parts of Chinese waxgourd seed; 7-16 parts of Lonicera japonica; 9-13 parts of Patrinia scabiosifolia; 8-15 parts of Homalomena occulta; 10-16 parts of Cyathocline purpurea; 7-15 parts of Mazus japonicas; 6-14 parts of broom cypress fruit; 9-15 parts of Cerasus pseudocerasus leaf; 7-12 parts of Pogostemon auricularius; 10-15 parts of Desmodium microphyllum; 8-13 parts of Colysis digitata leaf; and 9-12 parts of japanese nanocnide herb. The oral prescription provided by the invention has the efficacy of clearing heat and removing toxin, eliminating dampness and promoting urinary discharge, reinforcing kidney and strengthening resistance, promoting blood circulation and removing stasis, effectively improves the immunity of the organism, and has significant urethritis treatment effect. Also, the oral prescription provided by the invention is made from common Chinese herbs, and has no side effect.

The invention discloses an SSR marker for identifying germplasm of prunus pauciflora, application and an identifying method, and belongs to the technical field of plant molecular marker assisted germplasm resource identification and breeding. The SSR marker 78A9-1 is used as a molecular marker, genome DNA of germplasm of prunus pauciflora and affinis specifies is extracted, a pair of SSR marker primers is used for PCR amplification, an amplified product is subjected to polyacrylamide gel electrophoresis, spectrum bands are read, and the statistical analysis is performed. The invention overcomes the defect that the germplasm of the prunus pauciflora cannot be identified accurately, and provides the SSR marker for rapidly and accurately identifying the germplasm of the prunus pauciflora. TheSSR marker has the advantage of quickly and accurately identifying the germplasm of the prunus pauciflora.

The invention discloses a staggered-layer cultivation method for prunus avium and a four-layer arc-shaped shed frame used in the method. The staggered-layer cultivation method comprises the steps thatthe four-layer shed frame is set up in a field planting cherry orchard, odd-layer trees and even-layer trees are arranged in the same row at intervals, the main branches of the odd-layer trees grow on frame surfaces of shed frame bodies in the first layer and the third layer, the main branches of adjacent even-layer trees grow on a frame surfaces of shed frame bodies in the second layer and the fourth layer, and the odd-layer trees and the even-layer trees are alternately planted in staggered layers. The four-layer shed frame comprises upright columns 1 which are arranged vertically and horizontally, the two or more upright columns 1 are arranged into one row and fixedly buried, the upright columns 1 are each provided with four layers of arc-shaped cross arms 2, and the arc-shaped cross arms, in the same layer, on each row of upright columns 1 are connected through a pull rope into a shed frame surface. According to the cultivation method, staggered-layer cultivation is adopted, the tree structure is simpler and more convenient to manage, and production is early and stable. The four-layer arc-shaped shed frame is firm in structure and simple and flexible in building method, the management work spacer of flowers and fruits in the orchard can be controlled, and the orchard management cost is helped to be reduced.

The invention relates to the technical field of molecular markers, and particularly relates to a molecular marker for predicting Meloidogyne incognita resistance of Prunus sogdiana. The invention provides a primer for detecting the molecular marker, and the primer has a nucleotide sequence shown as SEQ ID No. 1-2. A method for predicting Meloidogyne incognita resistance of Prunus sogdiana by using the primer provided by the invention comprises the following steps of: extracting RNA of leaves of Prunus sogdiana and reversing the RNA into cDNA; performing PCR amplification by using the primer shown as SEQ ID No. 1-2; and detecting a PCR product by agarose gel electrophoresis. According to the invention, resistance evaluation is carried out without inoculating Meloidogyne incognita, resistant plants can be screened out, and important scientific guiding significance is provided for identification and variety pre-selection of excellent rootstocks resistant to Meloidogyne incognita.

The invention relates to a compound oil health food which is characterized by comprising the following raw materials in parts by weight: 20-50 parts of seahero colza oil, 10-30 parts of prunus pseudocerasus seed oil, 10-30 parts of grape seed oil, 10-20 parts of apricot kernel oil, 5-10 parts of tomato seed oil and 0.01-0.02 part of antioxidant. By fully utilizing the health effects of the raw materials and carrying out scientific formulation, the compound oil health food has balanced nutrition components and convenient taking, can fully utilize the compounded effective nutrition components thereof and is beneficial to body health after being taken for a long term.

The invention is applicable to the technical field of prunus humilis bunge beverages, and provides a preparation method of a prunus humilis bunge juice beverage. The prunus humilis bunge juice beverage takes prunus humilis bunge, hawthorn and cherry as main raw materials, so that the beverage disclosed by the invention has good taste and rich nutrition, and the beverage is rich in nutrition. In the preparation process, the probiotics with higher activity can be obtained by intensively selecting the probiotics, so that the fermentation speed of the fruit juice can be increased, the production time of the fruit juice can be shortened, meanwhile, the beverage added with the probiotics can promote intestinal digestion, solve constipation and facilitate nutrient absorption, and in the sterilization process, the packaged product is sterilized through water vapor and ultrasonic waves, the sterilization time can be shortened, nutrients and effects of the beverage are kept, and the production time of fruit juice is further shortened.

The invention discloses Prunus pseudocerasus L. DELLA proteins as well as coding genes and an application thereof in the technical field of conservation and utilization of genetic resources of agricultural wild plants. In the invention, key negative regulator DELLA proteins and the coding genes thereof in a gibberellin signal transduction path can be obtained by clone from cherries at the first time, which is called as PpGAI. The PpGAI is transformed into Escherichia coli BL21 so as to prove that the PpGAI has bioactivity and can code proteins with expected molecular weight sizes; and the PpGAI genes are transformed into wild arabidopsis thaliana so as to prove that the DELLA proteins of the coding genes can result in the obvious dwarf character of the arabidopsis thaliana. According to the invention, excellent dwarf genes are provided for fruit tree genetic engineering, and a new path is created for the dwarf and thick planting of the cherries.

A new and distinct cultivar of Prunus mahaleb is provided. The new cultivar is particularly well suited for serving as an understock during cherry production. A number of advantages are provided when compared to the standard Mahaleb rootstock. The cultivar is readily amenable to vegetative propagation (e.g., by the use of softwood cuttings), and exhibits some improved resistance to Phytophthora spp. When used with a `Bing` cherry scion, increased yields and yield efficiency have been observed without reduced fruit size. Improved precocity in bearing is displayed when compared to the standard Mahaleb rootstock. The new cultivar when grown without use as an understock forms a larger and more vigorous tree than the `UCMH 55` and `UCMH 56` cultivars that were products of the same research program. When used as a rootstock, the new cultivar produces a compact tree with a size reduction of approximately 20 to 25 percent compared to that produced when using a standard Mahaleb rootstock. Few suckers are produced.

The invention discloses low-sugar fruit candies which comprise the following raw materials in parts by weight: 60-100 parts of xylitol, 6-8 parts of blueberries, 10-12 parts of pineapple, 4-6 parts of fructus crataegi, 6-8 parts of Chinese chestnuts, 5-7 parts of prunus pseudocerasus, 2-4 parts of walnut kernels and 60-70 parts of water. The low-sugar fruit candies are peculiar in taste, sour, sweet and tasty, and low in sugar content.

The invention relates to the field of food and medicine, in particular to an application of a prunus cerasifera product in inhibition of thrombosis. The prunus cerasifera product comprises jam, dried fruits, preserved fruits, fruit juice, fruit powder, fruit wine, candies, pastries, oral liquid, fresh prunus cerasifera fruits and the like. Animal experiments show that the antithrombotic capability of a prunus cerasifera extract is equivalent to that of aspirin, and thrombosis caused by adenosine diphosphate or carrageenan can be effectively prevented and treated.