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Chinese toon tissue-culture quick propagation technique

A technology of tissue culture rapid propagation and Toona sinensis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of ensuring germplasm purity, enhancing resistance, and maintaining excellent seed properties

Inactive Publication Date: 2011-04-20
周玉玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, the conventional method of producing Chinese toon seedlings was: ① Propagate with root system, but due to too few roots, the amount of seedlings raised is low, and it can only be bred once a year. Slowness; ②The use of seeds to propagate seedlings will cause inconsistencies in the internal and external shapes and growth rates of individual plants, resulting in large differences in quality and nutrient content

Method used

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Examples

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Embodiment Construction

[0023] This embodiment is an example of tissue culture and rapid propagation using the "Red Toon No. 1" vegetable and material named after Henan Province.

[0024] 1. Differentiation induction culture

[0025] From the growing plants, select the plants that are robust, free from diseases and insect pests, and have the characteristics of this variety, cut off the branches with full semi-lignified axillary buds, remove the visible leaves, and wash them in running tap water for 20-30mis. dry moisture. On the ultra-clean workbench in the aseptic room, treat with 75% alcohol for 30S, sterilize with 0.1% mercuric chloride for 8-12min, rinse with sterile water for 5-7 times, and then cut into 1.0-1.5cm long The stem segment containing 1-2 axillary buds is quickly inoculated into the differentiation induction medium, sealed and marked, and quickly transferred to the culture room for differentiation induction culture.

[0026] The differentiation induction medium is prepared by addin...

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Abstract

The invention relates to Chinese toon tissue culture and rapid propagation technology. The technology comprises the following steps: clipping an annual semi-lignified branch with plump axillary buds from a growing plant, clipping the branch into a stem section containing 1 to 2 axillary buds after sterilization, and rapidly inoculating the stem section to a differentiation induction culture medium for differentiation culture; placing the plant obtained through the differentiation culture into a propagation culture medium for propagation and rapid propagation; and when a certain quantity is reached through the propagation, putting the plant into a rootage culture medium for sound seedling rootage culture, and hardening the seedling after the sound seedling takes root. The method carries out the asexual tissue rapid propagation and disinfection through the stem apex and stem section of Chinese toon. The propagated filial generation maintains the good seed nature of the original variety, ensures the seed purity and has no diseases and insects. The technology overcomes the defect of low propagation speed lying in the conventional seed and cut root propagation, enables the artificial control on the culture and growth conditions, is not limited by natural conditions, and has a small amount of drawn material. The technology can realize the industrialized seedling culture and high-efficiency production.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation technology of plants, specifically a tissue culture and rapid propagation technology of Chinese toon, and the proportion of medium used for breeding. Background technique [0002] Plant tissue culture refers to the process in which all organs, tissues and cells of a plant are placed in a medium containing plant nutrients and regulators under artificial control conditions to allow them to grow and differentiate to form a complete plant. The theoretical basis is the totipotency of plant cells. Plant tissue culture and rapid propagation technology has developed rapidly in recent years, and has been widely used in flowers, fruits, vegetables and crops, and has produced huge economic benefits. [0003] The culture medium is the key to the rapid propagation of plants. The ratio of the culture medium varies with different crops and with different growth and development stages of plants. Differ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周玉玲余慧琳孟宪政刘广卿谢振肖召杰石红梅张福娟姜曙光孙凤岭张家玉任德超李伟
Owner 周玉玲
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