Chinese toon tissue-culture quick propagation technique
A technology of tissue culture rapid propagation and Toona sinensis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of ensuring germplasm purity, enhancing resistance, and maintaining excellent seed properties
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[0023] This embodiment is an example of tissue culture and rapid propagation using the "Red Toon No. 1" vegetable and material named after Henan Province.
[0024] 1. Differentiation induction culture
[0025] From the growing plants, select the plants that are robust, free from diseases and insect pests, and have the characteristics of this variety, cut off the branches with full semi-lignified axillary buds, remove the visible leaves, and wash them in running tap water for 20-30mis. dry moisture. On the ultra-clean workbench in the aseptic room, treat with 75% alcohol for 30S, sterilize with 0.1% mercuric chloride for 8-12min, rinse with sterile water for 5-7 times, and then cut into 1.0-1.5cm long The stem segment containing 1-2 axillary buds is quickly inoculated into the differentiation induction medium, sealed and marked, and quickly transferred to the culture room for differentiation induction culture.
[0026] The differentiation induction medium is prepared by addin...
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