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Tissue Culture and Rapid Propagation Method of Cherry Plum

A tissue culture rapid propagation and cherry technology, which is applied in the field of agricultural biology, can solve the problems of low rooting rate of cherry plums, cannot guarantee the uniformity of seedlings, etc., and achieves the effects of fast reproduction speed, good effect of increasing height and excellent seed quality.

Active Publication Date: 2021-08-31
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, seed reproduction is sexual reproduction, and traits will be separated, so the uniformity of seedlings cannot be guaranteed. Cutting propagation is asexual reproduction, which can ensure the uniformity of seedlings, but the rooting rate of cherry plums is low.

Method used

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  • Tissue Culture and Rapid Propagation Method of Cherry Plum
  • Tissue Culture and Rapid Propagation Method of Cherry Plum
  • Tissue Culture and Rapid Propagation Method of Cherry Plum

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The establishment of embodiment 1 cherry plum tissue culture rapid propagation method

[0040] The present embodiment provides the rapid propagation method of cherry plum tissue culture, and its flow process is as follows figure 1 shown.

[0041] 1. Explant collection

[0042]Using the current-year-old stems of cherry plum seedlings as explants, the robust, semi-lignified middle stems of the current-year shoots were collected in June, and the leaves at the stem nodes were removed and rinsed with tap water. The upper part of the axillary bud is about 0.2 cm and the lower part is about 1.5 cm, respectively, and the stem section with the axillary bud is obtained, which is placed in a petri dish for later use;

[0043] 2. Disinfection of explants

[0044] Soak the stem section with axillary buds obtained on the ultra-clean workbench with 75% alcohol for 15 seconds, then soak for 15 minutes with 3% sodium hypochlorite, rinse 6 times with sterile water, and dry the moistur...

Embodiment 2

[0076] Example 2 Rapid Propagation of Cherry Plum Varieties "Senguo Honglu" and "Senguo Jiaren"

[0077] Utilize the Tissue Culture Rapid Propagation Method of Cherry Plum established in Example 1 to carry out rapid propagation respectively to Cherry Plum varieties "Senguo Honglu" and "Senguo Beauty", the specific methods are as follows:

[0078] The current year's stems of "Senguo Honglu" and "Senguo Jiaren" were taken as explants, and the robust, semi-lignified stems in the middle of the new shoots were collected, and the leaves at the stem nodes were removed and rinsed with tap water. Clean, cut off about 0.2 cm above and 1.5 cm below the axillary buds of each stem node to obtain stem segments with axillary buds, and put them in a petri dish for later use;

[0079] Step 1, soak the obtained stem section with axillary buds with 75% alcohol for 15 seconds, then soak it with 3% sodium hypochlorite for 15 minutes, rinse it with sterile water for 6 times, dry the surface moistur...

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Abstract

The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culture and rapid propagation of cherry plums. The specific steps of the method are as follows: sterilize and sterilize the stem section of the axillary buds of the cherry plum as explants, first inoculate them on the bud induction medium for primary culture to obtain the axillary buds, and then inoculate the axillary buds on the proliferation medium for propagation and culture , the multi-cluster buds obtained from multiplication were cut and divided into 2-4 small parts and inoculated into GA-containing 3 Heightening culture was carried out on the heightening medium, and then the sterile seedlings with a height of more than 2 cm were excised and inoculated on the rooting medium to take root, and the remaining low and dense multi-cluster buds were subcultured on the proliferation medium and cycled Wait until the aseptic seedling base on the rooting medium grows white taproots and some fibrous roots, then harden the seedlings, transplant them into the substrate, and obtain seedlings of cherry plums. The tissue culture rapid propagation method of the present invention is easier to obtain strong seedlings than single-use multiplication culture, and the multiplication coefficient is more than 3 times higher than that of single-use increased culture.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culture and rapid propagation of cherry plums. Background technique [0002] Cherry plum (scientific name: Prunus cerasifera Ehrhart) is a plant of the genus Prunus in the Rosaceae family. It is a precious wild fruit tree resource in Ili wild fruit forest in Xinjiang. extremely important conservation value. However, at present, wild cherry plum resources continue to decrease and are on the verge of extinction and urgently need to be protected. Cherry plums have strong stress resistance and can be used as rootstocks for cultivated fruit trees such as peaches, plums, and apricots. Through cultivation and utilization, the protection and utilization of cherry plum germplasm resources can be realized. Cherry plums can be propagated by seeds, cuttings, etc. But seed propagation is sexual reproduction, character separation can occur, and the u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 冀晓昊王海波刘凤之王孝娣王莹莹
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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