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Asexual propagation method of proserpinaca palustris

A technology of asexual reproduction and red umbrella, applied in the field of plant tissue culture, can solve the problems of difficult reproduction and slow reproduction, and achieve the effects of short tissue reproduction cycle, convenient operation, and simple and easy tissue culture method.

Active Publication Date: 2020-02-28
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is easy to operate, easy to operate, high in output, and overcomes the problems of difficult reproduction and slow reproduction speed in aquariums.

Method used

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  • Asexual propagation method of proserpinaca palustris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1: Prepare induction medium and proliferation medium

[0023] 1. The induction medium and proliferation medium used in this example are both MS medium adopted as basal medium, and the formulation of MS medium is as follows: mg / L

[0024] Table 1 MS medium formula

[0025] serial number components Content (mg / L) serial number components Content (mg / L) 1 KNO 3 :

1900 11 CuSO 4 .5H 2 o

0.025 2 NH 4 NO 3

1650 12 CoCL 2 .6H 2 o

0.025 3 MgSO 4 ·7H 2 o

370 13 Na 2 -EDTA

37.3 4 K H 2 PO 4

170 14 FeSO 4 ·7H 2 o

27.8 5 CaCl 2 2H 2 o

440 15 Glycine 2.0 6 MnSO 4 4H 2 o

22.3 16 Pyridoxine hydrochloride 0.5 7 ZnSO4 7H 2 o

8.6 17 Ammonium sulfate hydrochloride 0.1 8 h 3 BO 3

6.2 18 niacin 0.5 9 KI 0.83 19 Creatine 100 10 Na 2 MoO 4 ·7H 2 o

0.25

[0026] 2. Preparation of indu...

Embodiment 2

[0040] Step 1: Prepare Induction Medium and Proliferation Medium

[0041] 1. The induction and proliferation medium prepared in this example is the MS medium used as the basal medium, and the formulation of the MS medium is the same as that shown in Example 1.

[0042] 2. Preparation of induction medium

[0043] The induction medium is based on MS as the base medium, and 6-aminopurine (6-BA), agar powder, sucrose, activated carbon and plant tissue antibacterial agent (PPM) are added; the component concentration of the induction medium is: MS base medium 11.74g / L, 1.5mg / L 6-BA, 6g / L agar powder, 30g / L sucrose, 4g / L activated carbon, 1ml / LPPM.

[0044] 3. Proliferation medium preparation

[0045] Proliferation medium is based on MS as the basal medium, and 6-BA, indole acetic acid (IBA), agar powder and sucrose are added; the component concentration of the proliferation medium is: MS basal medium 11.74g / L, 5mg / L 6-BA, 0.75mg / LIBA, 6g / L agar powder and 30g / L sucrose.

[0046]...

Embodiment 3

[0056] Step 1: Prepare Induction Medium and Proliferation Medium

[0057] 2. The induction and proliferation medium prepared in this example is the MS medium used as the basal medium, and the formula of the MS medium is the same as that shown in Example 1.

[0058] 2. Preparation of induction medium

[0059] The induction medium is based on MS as the base medium, and 6-aminopurine (6-BA), agar powder, sucrose, activated carbon and plant tissue antibacterial agent (PPM) are added; the component concentration of the induction medium is: MS base medium 11.74g / L, 2mg / L 6-BA, 8g / L agar powder, 30g / L sucrose, 8g / L activated carbon, 1ml / LPPM.

[0060] 3. Proliferation medium preparation

[0061] Proliferation medium is based on MS as the basal medium, and 6-BA, indole acetic acid (IBA), agar powder and sucrose are added; the component concentration of the proliferation medium is: MS basal medium 11.74g / L, 2.5mg / L 6-BA, 1mg / LIBA, 8g / L agar powder and 30g / L sucrose.

[0062] Step 2...

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Abstract

The invention relates to an asexual propagation method of proserpinaca palustris. The method includes: (1), pre-treating an explant; (2), cutting the explant after being pre-treated into a plurality of small explants, and inoculating the small explants onto an induced culture medium for culture until clustered shoots are formed, wherein each small explant has 1-3 stem nodes; (3), separating the clustered shoots in the induced culture medium to control length of the clustered shoots to be 2-3cm; inoculating the clustered shoots after being separated into a proliferation culture medium for culture, separating new shoots when growing to 2-3cm, inserting the new shoots into the proliferation culture medium, and repeating subculture for 3-4 times to obtain clustered shoots after large-scale propagation; (4), splitting down the clustered shoots of 3-5cm in length in the clustered shoots after propagation from bases to shoot tubers, cutting, and planting. By the method, a lot of new plants can be obtained without being subject to season, temperature and regional impact, so that a proserpinaca palustris sterile propagation system can be established quickly, technical support can be provided for maintaining excellent seed characteristics of proserpinaca palustris, and ceaseless sterile seedlings are provided for the majority of fans.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for asexual reproduction of red umbrellas. Background technique [0002] Red Umbrella, dicotyledonous plant, Xiao Erxian grass family, emergent aquatic plants. There are different types of water grass and water grass. Water grass has lanceolate alternate leaves, leaf color is emerald green, leaf edge has serration. Cultivated in water, there will be many different changes. Including leaves can change from lanceolate to oval, irregularly split leaves, and even pinnate leaves. The color can change from green to orange-red to deep red. It varies according to the cultivation conditions, and it has a variety of forms. It likes strong light and low water temperature, and grows slowly in water. Rare things are more expensive, so red umbrellas are often regarded as classics among red grasses by aquatic plant players, and red umbrellas can change into different...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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