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Barbadosnut plantlet tissue culture rapid propagation and rooting method

A fast technology of Jatropha curcas, applied in the field of rapid propagation of Jatropha curcas plant tissue culture, can solve the problems of high cost, time-consuming and laborious, low regeneration rate, etc., and achieves the maintenance of excellent species, high reproductive coefficient, and low probability of variation. Effect

Inactive Publication Date: 2009-11-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of cost that exists in the existing Jatropha curcas tissue culture method, time-consuming and laborious, the problems such as regeneration rate is not high, and the rooting problem of Jatropha curcas tissue culture seedling that exists all the time in this field. The method for fast propagation and rooting of Jatropha curcas micro-seedling tissue culture is to lay the foundation for the promotion and application of Jatropha curcas tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Rinse the mature and shelled Jatropha curcas seeds repeatedly with clear water for 5 times, then soak them in clear water for 3 hours, then move them into the ultra-clean workbench and treat them with alcohol with a volume concentration of 75% for 50 seconds, rinse them with sterile water for 3 times, and then wash them with a mass concentration of 0.5% % mercuric chloride solution for 15 minutes, rinsed with sterile water for 3 times.

[0023] Take out the embryos from the sterilized seeds and place them in the basal medium MS+30g / L sucrose+5g / L agar at pH 5.4, and then culture them for 5 days at a culture temperature of 25°C, with a light intensity of 1500lx and a light time of 12h / d. Embryos germinate into sterile seedlings.

[0024] Cut the aseptic seedlings into single-bud stem segments and place them in the rapid propagation medium MS+0.1mg / L 6-BA+0.15mg / L IBA+25g / L sucrose+5g / L agar at pH 5.4. Clustered buds can be induced after 15 days of culture at 25°C, light...

Embodiment 2

[0027] Rinse the mature and shelled Jatropha curcas seeds repeatedly with clean water for 4 times, then soak them in clean water for 3 hours, then move them into the ultra-clean workbench and treat them with 70% alcohol in volume concentration for 30 seconds, rinse them with sterile water for 2 times, and then wash them with a mass concentration of 0.4 % mercuric chloride solution for 17 minutes, rinsed with sterile water for 3 times.

[0028] The embryos in the sterilized seeds were taken out and placed in the basal medium MS+30g / L sucrose+5g / L agar of pH 5.6, and then cultured at a culture temperature of 27°C and a light intensity of 2000 lx for 5 hours at a light time of 12h / d. Embryos germinated into sterile seedlings.

[0029] Cut the aseptic seedlings into single-bud stem segments and place them in the rapid propagation medium MS+0.3mg / L 6-BA+0.15mg / L IBA+30g / L sucrose+5g / L agar of pH5.6. The temperature is 27°C, the light intensity is 2000lx, and the light time is 12h / ...

Embodiment 3

[0032] Rinse the mature and shelled Jatropha curcas seeds repeatedly with clear water for 4 times, then soak them in clear water for 3 hours, then move them into the ultra-clean workbench and treat them with 80% alcohol by volume concentration for 1 minute, rinse them with sterile water for 3 times, and then use them with a mass concentration of 0.5 % mercuric chloride solution for 10 minutes, rinsed with sterile water twice.

[0033] The embryos in the sterilized seeds were taken out and placed in the basal medium MS+35g / L sucrose+5g / L agar of pH 5.8, and then cultured at a culture temperature of 29°C and a light intensity of 2500lx, and the light time was 12h / d for 5 Embryos germinated into sterile seedlings.

[0034] Cut the aseptic seedlings into single-bud stem segments and place them in the rapid propagation medium MS+0.5mg / L 6-BA+0.15mg / L IBA+35g / L sucrose+5g / L agar of pH5.8. The temperature is 29°C, the light intensity is 2500lx, and the light time is 12h / d. After 7 d...

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Abstract

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of Jatropha curcas plant tissue culture rapid propagation, in particular to a method for tissue culture rapid propagation and rooting of Jatropha curcas micro seedlings. technical background [0002] Jatropha curcas is a plant of the Euphorbiaceae Jatropha genus, mainly distributed in tropical and subtropical regions. Jatropha curcas has the effect of dispelling silt and relieving pain, and is often used to treat bruises, skin itching and gastroenteritis. In recent years, studies have found that Jatropha curcas not only contains anti-cancer, disease-resistant, and insect-resistant ingredients, which has potential important value in the development of new drugs, but also has a high oil content, and its oil composition is close to that of petrochemical diesel oil. Jatropha curcas is resistant to drought and barrenness, and has low environmental requirements. Therefore, Jatropha curcas can be used as an energy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王胜华宗桦陈放
Owner SICHUAN UNIV
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