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57results about How to "Single type" patented technology

Human chorion mesenchymal stem cell isolated culture method

InactiveCN105713871AEasy to handleReduce the probability of failure of isolation cultureCell dissociation methodsSkeletal/connective tissue cellsTissue CollectionMesenchymal stem cell
The invention relates to a method for separating and culturing human chorionic mesenchymal stem cells, belonging to the technical field of biomedicine. The present invention obtains mesenchymal stem cells and in vitro culture and freezing of mesenchymal stem cells through the collection of human placental chorionic tissue, stripping of chorionic tissue, disinfection, washing, shredding, primary separation, and the isolated cells are related detection. The invention can separate and obtain a large number of mesenchymal stem cells with uniform and stable phenotype from human placental chorionic tissue, which meets clinical application standards through testing. The number of cells that can meet cryopreservation or clinical use can be obtained by culturing in vitro expansion.
Owner:YUNNAN SHUNXI REGENERATION MEDICAL ENG CO LTD

Method for separating and purifying chlorogenic acid from eucommia bark leaves

The invention discloses a method for separating and purifying chlorogenic acid from eucommia bark leaves, which comprises the following steps of: extracting eucommia bark plant leaves serving as raw materials at the temperature of between 40 and 70 DEG C for 15 to 40 minutes by using 20 to 80 volume percent aqueous solution of a strongly polar organic solvent as an extractant with the aid of microwaves with the power of 300 to 800W, filtering extract, and drying to obtain a eucommia bark leaf flavonoid crude product, wherein the material-to-liquid ratio of the raw materials to the extractant is 1g:(10-50)mL; and performing high-speed countercurrent chromatographic separation and purification on the eucommia bark leaf flavonoid crude product by using ethyl acetate, n-butanol and water in avolume ratio of (3-4):1:(4-5) as a solvent system to obtain the chlorogenic acid. The invention has the main advantages that: operating steps are simple, the equipment type is single, the production period is short, product purity is high, and the method has high application value and good social and economic benefits.
Owner:ZHEJIANG UNIV OF TECH

Method for obtaining germination effective symbiotic fungus for Cymbidium bicolor seeds

The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.
Owner:JINGHONG HONGZHEN AGRI TECH CO LTD

Barbadosnut plantlet tissue culture rapid propagation and rooting method

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.
Owner:SICHUAN UNIV

Low-creep azorite indecomposable overflow brick and preparation method thereof

ActiveCN105218121AReduce the temperatureReduced fire resistancePorosityBrick
The invention relates to a low-creep azorite indecomposable overflow brick and a preparation method thereof. The brick comprises the following raw materials in percentage by weight: 99.40-99.85% of azorite powder and 0.15-0.60% of ilmenite powder. The product prepared by the method provided by the invention is high in volume density and extremely low in apparent porosity, significantly reduces the high-temperature bending creep rate of an azorite material, and has an extremely-low foaming index to liquid crystal glass; and the preparation method is small in added quantity of a sintering aid, does not cause decomposition of azorite during firing, is simple and easy to implement, is scientific and reasonable, and is convenient for large-scale industrial production.
Owner:ZIBO GT INDAL CERAMICS

Tumble detection method and device

The invention discloses a tumble detection method and device. A first pressure signal, a second pressure signal and a third pressure signal are acquired in real time through a first pressure sensor arranged on the inner side of a shoe sole, a second pressure sensor arranged on the inner side of a shoe rear side part, and a third pressure sensor arranged on the inner side of a shoe heel part respectively; if the duration time of the acquired first pressure signal, second pressure signal and third pressure signal meeting the following conditions reaches a preset time threshold value: the first pressure signal is smaller than a first preset threshold value, or the second pressure signal is larger than a second preset threshold value, or the third pressure signal is larger than a third preset threshold value, it is judged that a tumble happens; a tumble alarm signal is given out under the condition that it is judged that the tumble happens. The tumble can be accurately detected, the implementation method is simple, and cost is low.
Owner:GUANGZHOU SHIYUAN ELECTRONICS CO LTD

Method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation

The invention discloses a method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation. The method comprises the following steps of: taking the explant of millettia dielsiana and cultivating the explant on a callus induction culture medium, thereby obtaining non-embryogenic callus; carrying out multiplication culture of the non-embryogenic callus on a callus multiplication culture medium, thereby obtaining a rapidly grown, loose cell aggregate; inoculating the cell aggregate to a cell suspension starting culture medium for cultivation, thereby obtaining a liquid cultured single cell suspension system; after centrifugation of the single cell suspension system, inoculating the precipitate to a liquid multiplication culture medium, thereby obtaining suspension cells; extracting the suspension cells with water and then adding absoluteethylalcohol to the extract until the volume percent content of ethanol in the solution is 60-75%, and carrying out rotary evaporation on the supernate, thereby obtaining the coarse beautiful millettia root polysaccharides. The method provided by the invention has the characteristics of short production period, no occupation of cultivated land, stable yield, high active ingredient content and no damage on natural resources, and therefore, the method has great development and utilization value.
Owner:TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

Cloud terminal processing method and system based on read-write separation

The invention specifically relates to a cloud terminal processing method and system based on read-write separation. The technical problem to be solved is to provide the cloud terminal processing method and system which can realize the read-write separation at an operating system level, do not need to change the configuration of a read-write area, cannot affect the operation of a cloud terminal, and are based on the read-write separation. The adopted technical scheme includes the following steps: partitioning a storage device to obtain a rootfs image and a boot partition image; establishing anOverlay partition; re-combining the rootfs image subjected to mirroring by the system with the Overlay partition to obtain a file system with read-write separation; and mounting the file system with read-write separation. The invention is applicable to the field of computers.
Owner:JIANGSU MORNING CLOUD INFORMATION TECH

Raw bamboo multi-pipe beam space lattice structure system and double-pipe-clamp type connecting element series

The invention discloses a raw bamboo multi-pipe beam space lattice structure system and a double-pipe clamp type connecting element series. A single double-pipe clamp comprises a left circular ring, a right circular ring and screw bolts; the left and right circular rings have the same or different dimensions, are respectively hooped on the outer wall of one bamboo pipe and are tightly hooped and fixed by screw bolts arranged at the two sides; the sides between the left circular ring and the right circular ring are connected through being fastened and hooped by the same screw bolt; the single bamboo pipe triangular shape is mainly formed by connecting three bamboo pipes as rod elements by a folding type double-pipe clamp; the raw bamboo multi-pipe beam space lattice structure is mainly formed by connecting a plurality of bamboo pipe triangles as installing units by the double-pipe clamp; the bamboo pipe or the bamboo pipe triangular shape can be sequentially and singly replaced through dismounting and mounting the double-pipe clamp; the structure repair is completed. The structure of the double-pipe clamp type connecting element is simple; the universality is high; the processing convenience is good; the connection reliability of the bamboo pipe and the double-pipe clamp is high; the integral structure bearing force is great; the building use comfort is good; the structure repair is convenient; the structure durability is high.
Owner:ZHEJIANG UNIV

Human placenta mesenchymal stem cell, preparation method and application thereof

The invention provides a human placenta mesenchymal stem cell, a preparation method and an application thereof. The preparation method comprises the following steps: collecting, separating, culturing,cryopreserving, detecting, recovering, and the like. A high-purity high-activity mesenchymal stem cell can be acquired by only performing slide adherent culture after a human placenta chorion tissueblock is acquired in the separating process; the technological process is simplified; the cost of enzymic digestion is saved; the adherence and growth rate of primary cells are accelerated; the cell culture period is shortened; the introduction of more external interference factors is avoided, so that the process stability can be easily controlled. The multiplication capacity of the human placentamesenchymal stem cell acquired according to the invention is more stable than that of other mesenchymal stem cells; after the human placenta mesenchymal stem cell passes to P20 generation, the cell still can stably proliferate, and the cellular morphology, molecular surface antigen and adipogenesis osteogenesis differentiative potential thereof all meet the regulations for minimum standard of MSCidentification from international cell therapy association.
Owner:山西省干细胞基因工程有限公司

Gannan potentilla anserine efficient planting method

The invention discloses a Gannan potentilla anserine efficient planting method. The method comprises the steps of land selection, land preparation and fertilization, plant disease and insect pest controlling and digging at a proper time; the planting method is characterized by five aspects of a planting season and seed selection, planting pattern, planting density and seeding quantity, an irrigation method and irrigation quantity and foliage application; according to an efficient planting technology of potentilla anserine in areas in hostile natural conditions which is high and cold and high in altitude and the like, scale and standardized potentilla anserine planting in the Gannan plateau area is achieved, the potentilla anserine industry is developed, and service guarantee is provided for economy development, targeted poverty alleviation, and income increase of farmers and herdsmen in high and cold areas in hostile natural environment conditions.
Owner:GUANGXI NORMAL UNIV FOR NATITIES

Catalyst for synthesizing 3-buten-1-ol through 1,4-butanediol and preparation method of catalyst

The invention provides a catalyst for synthesizing 3-buten-1-ol through 1,4-butanediol and a preparation method of the catalyst. The catalyst is a CaO-SiO2-ZrO2 catalyst prepared by combining sol-gel with deposition-precipitation through a one-pot process, wherein the content of CaO in the catalyst is 5-25wt%, and the content of SiO2 is 0-15wt%. The preparation method comprises the following steps: hydrolyzing a silicon source, hydrolyzing a zirconium source, gelling silicon-zirconium, performing calcium source deposition-precipitation and performing aftertreatment. The preparation method is simple, the operation is convenient, and large-scale production is easily realized. According to the prepared catalyst, 1,4-butanediol is catalyzed to perform selective dehydration to synthesize 3-buten-1-ol under the reaction condition of the temperature of 300-400 DEG C, the yield is 90 percent or above, and fewer reaction byproducts are produced and are easy to separate.
Owner:SHANXI UNIV

Mold used for manufacturing fan blade shearing ribs of wind generating set

The invention discloses a mold used for manufacturing fan blade shearing ribs of a wind generating set, which comprises a mold die cavity and a turning edge. The invention is characterized in the mold is made of steel. The mold used for manufacturing the fan blade shearing ribs of the wind generating set of the invention uses the steel mode, so the mold manufacture work procedure is simple, the materials have single type, the manufacture cost is lower, in addition, the later period maintenance and conservation cost is low, and the correction after the occurrence of deformation is easy.
Owner:昆山华风风电科技有限公司

Method for planting edible healthcare Gannan potentilla anserine

The invention discloses a method for planting edible healthcare Gannan potentilla anserine. The method comprises the following steps: selecting a land, and performing soil preparation and fertilization. The method comprises five key techniques of determining plant periods, selecting seeds, selecting planting modes, determining planting density and seeding amounts, selecting irrigation modes and irrigation amounts, and spraying fertilizers to leaves, and steps of preventing and treating insects and diseases and digging and harvesting Gannan potentilla anserine at proper time. The method is an efficient planting technique for planting potentilla anserine in regions with harsh natural conditions, such as high and cold places and high altitudes; large-scaleand normalized potentilla anserine planting in Gannan plateau regions can be achieved, related industries of the potentilla anserine can be developed, and service assurance can be provided for promoting economic development in high and cold places with harsh natural environment conditions, promoting targeted poverty alleviation, promoting targeted poverty overcome and increasing income of farmers.
Owner:GUANGXI NORMAL UNIV FOR NATITIES

Preparation method of two-dimensional carbon-rich material rich in carbonyl, thioketone and selenone functional groups and application of two-dimensional carbon-rich material

The invention discloses a preparation method of a two-dimensional carbon-rich material rich in carbonyl (double bonds of carbon to oxygen), thioketone (double bonds of carbon to sulphur) and selenone (double bonds of carbon to selenium) functional groups and application of the two-dimensional carbon-rich material, and belongs to the field of application of the material. The material consists of conjugated six-membered rings containing the carbonyl, thioketone and selenone functional groups and acetylenic bonds; 1 and 4 positions of each conjugated six-membered ring are substituted by the carbonyl, thioketone and selenone functional groups; 2, 3, 5 and 6 positions of each conjugated six-membered ring are connected with the acetylenic bonds; and two acetylenic bonds exist between each two adjacent six-membered rings to form a highly conjugated two-dimensional planar framework structure. The preparation method mainly comprises the following steps: taking materials such as tetra-alkynyl substituted p-benzoquinone as a polymeric precursor; carrying out alkyne coupled reaction in a dioxane solution so as to obtain the carbon-rich two-dimensional material with the highly conjugated structure. Owing to introduction of carbonyl, double bonds of carbon to sulphur and double bonds of carbon to selenium, an ion transport channel and an energy storage activity points of the two-dimensional carbon-rich material are improved effectively, and therefore, the two-dimensional carbon-rich material has good application prospect in the field of energy storage devices.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Officinal Gannan potentilla anserine planting method

The invention discloses an officinal Gannan potentilla anserine planting method. The officinal Gannan potentilla anserine planting method includes the steps of land selection, soil preparation and fertilization. The key technology of the planting method includes the steps of the planting stage and seed selection, the planting mode, the planting density and seeding rate, the irrigation mode and irrigation volume and foliage application; disease and insect damage preventing and controlling and timely digging are adopted. According to the efficient potentilla anserine planting technology for the high and cold high-altitude regions with bad natural conditions, potentilla anserine can be planted in the Gannan plateau region in a standard mode at a large scale, the potentilla anserine industry is developed, and service assurance is provided for economy developing, targeted poverty alleviation, targeted poverty overcoming and farmer and herdsmen income increasing of high and cold region with bad natural environmental conditions.
Owner:GUANGXI NORMAL UNIV FOR NATITIES

Preparation method of ramelteon

The invention belongs to the field of medicinal chemistry, and mainly relates to a (S)-2-(1,6,7,8-tetrahydro-2H-indeno[5,4-b]furan-8-yl)-1-ethylamine compound represented by a formula I. The method has the advantages that the operation is simple, the steps are reduced compared with the previous method, the route is shortened, and the used solvent is single in variety, convenient to recycle and high in yield, wherein the formula I is defined in the specification.
Owner:BEIJING VENTUREPHARM BIOTECH

Barbadosnut plantlet tissue culture rapid propagation and rooting method

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.
Owner:SICHUAN UNIV

Up-to-standard septic tank

The invention discloses an up-to-standard septic tank and aims to integrate pretreatment and deep treatment of living sewage. The up-to-standard septic tank mainly comprises a manure sludge tank, a tubular grid, an indirect backflow system and a biological contact oxidation filtering settling pond. Sewage is subjected to filtration, settling and biochemical action in the up-to-standard septic tank, pollutants are removed, the water dissolved oxygen is increased, and the sewage is directly discharged or recycled after corresponding standards are met. The investment and operation cost of the up-to-standard septic tank is increased to a certain extent when being compared with that of a conventional septic tank, but a conventional sewage treatment mode of 'septic tank+sewage collection pipeline net+concentrated sewage treatment' is changed, the investment of pipeline nets and sewage treatment plants is reduced, and sewage can be recycled on site conveniently.
Owner:邱学尧

Real-time monitoring system and method of intelligent visual three-dimensional geological sliding and collapsing

The invention relates to a real-time monitoring system and method of intelligent visual three-dimensional geological sliding and collapsing. A luminescent calibration plate is set in the monitoring area for long-distance projection, a camera with an MCU module is set up in a stable field and the camera completes image extraction of three light spots in long-distance projection. The displacement changes of the three light spots are monitored, the MCU operation is performed, and an RS485 and a Zigbee transmission interface are used to return a monitoring result to a remote terminal. The invention only needs a single camera module and three right-angled LED calibration points, and does not need image recognition. Only the images obtained before and after can be compared to calculate the displacement of the three-dimensional geological slide, which greatly simplifies the computational complexity. Therefore, the use of a high-speed microcomputer or a DSP chip is not required, the system architecture is simple, and the cost and the power consumption are low.
Owner:ZHANGZHOU EASTERN INTELLIGENT METER

Nickel/zirconia catalyst and its preparation method and method for preparing synthesis gas

The invention discloses a method for preparing a Ni / ZrO2 catalyst. The method comprises the following steps: (1) preparing zirconium salt microemulsion, nickel salt microemulsion and alkaline microemulsion respectively; salt microemulsion, nickel salt microemulsion and alkaline microemulsion are mixed; (3) the system obtained by mixing step (2) is subjected to solid-liquid separation to obtain a solid product, and the solid product is cleaned, dried and roasted to obtain Ni / ZrO2 catalyst. The invention also provides the Ni / ZrO2 catalyst prepared by the method and a method for preparing synthesis gas by autothermal reforming of methane. The preparation method of the Ni / ZrO2 catalyst of the invention has simple process, easy operation and low cost, and the prepared catalyst has excellent catalytic activity and stability in the reaction of methane autothermal reforming to prepare synthesis gas.
Owner:CHINA PETROLEUM & CHEM CORP +1

System and method for monitoring oil consumption of engineering vehicle and engine

The invention discloses a system and a method for monitoring oil consumption of an engineering vehicle and an engine. The system for monitoring the oil consumption of the engine comprises a first pressure acquiring device, a second pressure acquiring device, a third pressure acquiring device and a controller, wherein the first pressure acquiring device is used for acquiring a first pressure value of an inlet of a control oil circuit of a hydraulic control valve in a hydraulic pipeline connected between the engine and an oil pump; the second pressure acquiring device is used for acquiring a second pressure value of an outlet of an executing oil circuit of the hydraulic control valve in the hydraulic pipe connected between the engine and the oil pump; the third pressure acquiring device is used for acquiring a third pressure value of an oil outlet of the oil pump; and the controller is used for receiving the first pressure value, the second pressure value and the third pressure value and determining the oil consumption of the engine according to the first pressure value, the second pressure value and the third pressure value. Compared with the conventional power measuring system, the system for monitoring the oil consumption of the engine in an embodiment has the advantages that the oil consumption of the engine can be measured by the three pressure sensors, and the sensors are single in type and simple in arrangement mode.
Owner:SANY HEAVY IND CO LTD (CN)

System and method for calcining treatment and resource utilization of sugar-making filter mud

The invention provides a system for calcining treatment and resource utilization of sugar-making filter mud. The system comprises a pulverized coal preparation system, a feeding system, a calcining system, a waste heat recovery system, a flue gas environment-friendly up-to-standard emission treatment system and a quick lime recovery system, Raw material coal is prepared into pulverized coal as auxiliary fuel, crude sugar filter mud is crushed, the pulverized coal and the crude sugar filter mud are simultaneously added into a calcining furnace, ash generated after calcining is discharged and cooled to serve as a quick lime material; flue gas generated after calcination enters a waste heat boiler to be subjected to heat recycling, saturated steam is generated, the flue gas obtained after heat exchange enters a denitration device to be subjected to denitration treatment, the flue gas obtained after denitration exchanges heat with air and then is subjected to dust removal, and clean flue gas is obtained; and air heated by the flue gas is introduced into the calcining furnace as combustion-supporting air, so that the temperature of the combustion-supporting air entering the calcining furnace is integrally increased. Reduction and harmless treatment of the sugar-making filter mud are achieved, meanwhile, quick lime generated in the high-temperature calcination process, namely the high-temperature decomposition reaction process, is recycled to achieve the purpose of resource utilization, high-temperature flue gas waste heat generated in calcination is recycled to generate saturated steam, and the purpose of energy conservation in the environment-friendly treatment process is achieved.
Owner:大连科林能源工程技术开发有限公司

Preparation method of preserved Chinese cabbage

The invention discloses a preparation method of preserved Chinese cabbage, belonging to the technical field of food processing. The preparation method of the preserved Chinese cabbage comprises the steps of selecting a material, pricking, cleaning, cutting into pieces, hardening, soaking, cooking, soaking for a second time, drying, metering and packaging, wherein the bone soup raw material comprises one or more of pig bones or pork, beef bones or beef and the like. According to the preparation method disclosed by the invention, the preserved fruit is soaked in the bone soup, and the obtained preserved Chinese cabbage is delicious in taste, bright, transparent and convenient to eat, so that the blank of preserved vegetable products on the market can be filled up, the varieties of the preserved fruits can be increased, the types of the preserved fruit products are diversified, and the consumers can conveniently select in various aspects; furthermore, on the basis that the original nutritive value of Chinese cabbages is reversed, the nutrient substances such as a great deal of calcium and ossein in the bone soup enter the preserved fruit by soaking, so that the types and the content of the nutrient substances in the preserved Chinese cabbage are greatly enriched; the leisure preserved vegetable which is delicious in taste and has the functions of effectively preventing rarefaction of bone and constipation, delaying senescence, nourishing yin and nourishing blood can be provided for the consumers and is suitable for being eaten for a long time.
Owner:宁夏天瑞产业集团现代农业有限公司

Human FGFR1 gene copy number variation nucleic acid standard substance, preparation method thereof and kit

The invention discloses a human FGFR1 gene copy number variation nucleic acid standard substance, a preparation method thereof and a kit. The human FGFR1 gene copy number variation nucleic acid standard substance comprises background genome DNA and linearized plasmid DNA containing an FGFR1 complete genome, wherein the background genome DNA is extracted from a human normal B lymph suspension cell line. In the invention, the preparation of the background genome only needs to culture one cell line, the cell line is single in type and less in workload, and during mixing, different amounts of linearized plasmid DNA are respectively mixed into the background genome DNA, and the backgrounds of different gradient standard substances are consistent, so that CNV analysis of a high-throughput sequencing method is especially facilitated; and meanwhile, in the nucleic acid standard substance, a fragment inserted by the linearized plasmid DNA is the FGFR1 complete genome, the length reaches 100kbp or above, a real gene amplification situation can be simulated, the nucleic acid standard substance is suitable for various CNV detection methods, and the problem that the application of a standard substance for local region amplification is limited is solved.
Owner:北京求臻医疗器械有限公司

System and method for continuously preparing acetophenone

PendingCN114105742AAvoid washing to remove acid impuritiesSingle typeOrganic compound preparationChemical industryOil phaseAcetophenone
The invention provides a system and a method for continuously preparing acetophenone, which are characterized in that ethylbenzene is used as a raw material and is sequentially subjected to at least one stage of rectification reaction; in each stage of rectification reaction, the material is fully mixed with part of gas phase to enter a reaction rectification tower and is in contact reaction with gas phase rising from the bottom in the tower, a mixed gas phase mainly containing water and ethylbenzene is extracted from the tower top, condensation and phase splitting are performed, an oil phase in condensate returns to the tower top, a water phase in the condensate is extracted as wastewater, and the water phase in the condensate is recycled. One part of the reaction liquid extracted from the tower kettle is fed into the next-stage rectification reaction, the other part of the reaction liquid is returned to the upper part of the reactive distillation tower as a circulating material through temperature control, and the part of the reaction liquid extracted from the tower kettle in the last-stage rectification reaction, which is not used as the circulating material, is extracted as a product. The method has the characteristics of good reaction selectivity, no generation of hazardous waste, energy conservation, environmental protection, continuous operation, easiness in large-scale production and the like.
Owner:天津精分科技发展有限公司 +1

A kind of preparation method of preserved cabbage

The invention discloses a preparation method of preserved Chinese cabbage, belonging to the technical field of food processing. The preparation method of the preserved Chinese cabbage comprises the steps of selecting a material, pricking, cleaning, cutting into pieces, hardening, soaking, cooking, soaking for a second time, drying, metering and packaging, wherein the bone soup raw material comprises one or more of pig bones or pork, beef bones or beef and the like. According to the preparation method disclosed by the invention, the preserved fruit is soaked in the bone soup, and the obtained preserved Chinese cabbage is delicious in taste, bright, transparent and convenient to eat, so that the blank of preserved vegetable products on the market can be filled up, the varieties of the preserved fruits can be increased, the types of the preserved fruit products are diversified, and the consumers can conveniently select in various aspects; furthermore, on the basis that the original nutritive value of Chinese cabbages is reversed, the nutrient substances such as a great deal of calcium and ossein in the bone soup enter the preserved fruit by soaking, so that the types and the content of the nutrient substances in the preserved Chinese cabbage are greatly enriched; the leisure preserved vegetable which is delicious in taste and has the functions of effectively preventing rarefaction of bone and constipation, delaying senescence, nourishing yin and nourishing blood can be provided for the consumers and is suitable for being eaten for a long time.
Owner:宁夏天瑞产业集团现代农业有限公司

Multi-chip normally-mounted reset wafer level packaging structure and method

The invention discloses a multi-chip normally-mounted reset wafer-level packaging structure and method, and the method comprises the following steps: carrying out the reset layout of multiple chips, placing different types of chips 1 on a substrate 12 on which a temporary bonding film 11 is pasted through employing a paster technology, enabling the front surfaces of the chips to be upward, and enabling the back surfaces of the chips to be pasted on the temporary bonding film on the substrate; the front surface of the chip comprises electrodes and key functional areas; manufacturing a first insulating layer, namely manufacturing the first insulating layer 15 on the chip surface of the substrate 12 by utilizing coating, spraying and laminating processes, and performing patterning treatment by utilizing processes such as photoetching and the like to expose part of electrodes and all key functional areas of the chip; and finally, after the rewiring process is completed, the main packaging process is completed, and then the chips are cut into single chips according to the size of each group of chips for subsequent PCB welding. The multi-chip packaging technology belongs to one-time packaging of the multiple chips, the packaging process of the multiple chips is simplified, and the packaging efficiency is improved.
Owner:苏州科阳半导体有限公司

Method for separating and recycling positive and negative electrode mixed powder of waste ternary lithium ion battery

The invention provides a method for separating and recycling positive and negative electrode mixed powder of a waste ternary lithium ion battery. The method comprises the following steps of: screening the positive and negative electrode mixed powder of the waste ternary lithium ion battery, and stirring and dispersing to obtain slurry I; adding a flocculating agent into the slurry I, carrying out bridging flocculation under the condition of a low-intensity magnetic field to obtain slurry II containing a large amount of floccules, and then adding magnetic seeds for further magnetization to obtain floc slurry III containing a large amount of magnetic floccules containing the magnetic seeds; treating the slurry III by using high-intensity magnetic separation equipment to separate out a non-magnetic material and a magnetic material; and filtering and pyrolyzing the non-magnetic material at high temperature to obtain recycled negative electrode graphite powder; and subjecting the magnetic material to ultrasonic treatment and then treating it by using low-intensity magnetic separation equipment to obtain a strong magnetic material and a low-intensity magnetic material which can be recycled as magnetic seeds, and filtering and pyrolyzing the low-intensity magnetic material at high temperature to obtain recycled high-purity positive electrode material powder. The method has the advantages of simple process, low wastewater and dust emission, small agent addition amount, recyclable magnetic seeds and high recovery quality.
Owner:SHENZHEN QINGYAN EQUIP TECH CO LTD

System and method for monitoring oil consumption of engineering vehicle and engine

The invention discloses an engineering vehicle, an engine oil consumption monitoring system and a method. Wherein, the engine oil consumption monitoring system includes: comprising: a first pressure obtaining device, which is used to obtain the first pressure value at the inlet of the control oil circuit of the hydraulic control valve in the hydraulic line connected between the engine and the oil pump; the second pressure obtaining device The device is used to obtain the second pressure value at the outlet of the executive oil circuit of the hydraulic control valve in the hydraulic line connected between the engine and the oil pump; the third pressure obtaining device is used to obtain the third pressure at the oil outlet of the oil pump value; a controller, configured to receive the first pressure value, the second pressure value and the third pressure value, and determine the fuel consumption of the engine according to the first pressure value, the second pressure value and the third pressure value. Compared with the existing power measurement system, the embodiment of the present invention can complete the measurement of engine oil consumption with three pressure sensors, the sensor type is single, and the arrangement is relatively simple.
Owner:SANY HEAVY IND CO LTD (CN)
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