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Method for separating and purifying chlorogenic acid from eucommia bark leaves

A technology for separation and purification of eucommia leaves, applied in the field of separation and purification of chlorogenic acid, which can solve the problems of low recovery rate of target components, poor separation and purification effect, low extraction rate of chlorogenic acid, etc., and achieve good social and economic benefits and short production cycle Effects of short, broad application values

Active Publication Date: 2011-05-04
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a method for separating and extracting chlorogenic acid from Eucommia leaves, which overcomes the low extraction rate of chlorogenic acid in Eucommia leaves, poor separation and purification effect, low recovery rate of target components and high purity. Not advanced defects

Method used

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  • Method for separating and purifying chlorogenic acid from eucommia bark leaves
  • Method for separating and purifying chlorogenic acid from eucommia bark leaves
  • Method for separating and purifying chlorogenic acid from eucommia bark leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Accurately weigh 30.0 g of eucommia leaf powder samples that have passed through an 80 mesh sieve, use 900 mL of 50% acetone aqueous solution as the extractant, feed the material according to the ratio of solid to liquid (g / mL) of 1:30, extract at 50 ° C for 25 min, and the microwave power is 600W. After the extraction was completed, it was vacuum filtered, and the filtrate was dried by rotary evaporation to obtain 3.8432 g of dried product, which was the crude product of eucommia flavonoids, with a yield of 12.81%.

Embodiment 2

[0036] Accurately weigh 5.0 g of eucommia leaf powder sample passed through an 80-mesh sieve, use 80 mL of 50% acetone aqueous solution as the extractant, feed the material according to the ratio of solid to liquid (g / mL) of 1:16, microwave power 400W, and extract at 40°C After 20 minutes, vacuum filtration was performed after the extraction was completed, and the filtrate was dried by rotary evaporation to obtain 5.7452 g of dried product, which was the crude product of eucommia flavonoids, with a yield of 11.49%.

Embodiment 3

[0038]Accurately weigh 5.0 g of eucommia leaf powder samples that have passed through an 80 mesh sieve, use 100 mL of 50% acetone aqueous solution as the extractant, feed the material according to the ratio of solid to liquid (g / mL) of 1:20, extract for 30 min at 50 ° C, and use a microwave power of 500W. After the extraction was completed, vacuum filtration was performed, and the filtrate was dried by rotary evaporation to obtain 0.6270 g of crude Eucommia flavonoids, with a yield of 12.54%.

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Abstract

The invention discloses a method for separating and purifying chlorogenic acid from eucommia bark leaves, which comprises the following steps of: extracting eucommia bark plant leaves serving as raw materials at the temperature of between 40 and 70 DEG C for 15 to 40 minutes by using 20 to 80 volume percent aqueous solution of a strongly polar organic solvent as an extractant with the aid of microwaves with the power of 300 to 800W, filtering extract, and drying to obtain a eucommia bark leaf flavonoid crude product, wherein the material-to-liquid ratio of the raw materials to the extractant is 1g:(10-50)mL; and performing high-speed countercurrent chromatographic separation and purification on the eucommia bark leaf flavonoid crude product by using ethyl acetate, n-butanol and water in avolume ratio of (3-4):1:(4-5) as a solvent system to obtain the chlorogenic acid. The invention has the main advantages that: operating steps are simple, the equipment type is single, the production period is short, product purity is high, and the method has high application value and good social and economic benefits.

Description

(1) Technical field [0001] The invention relates to a method for extracting and preparing active ingredients from plants, in particular to a method for separating and purifying chlorogenic acid from Eucommia leaves. (2) Background technology [0002] Eucommia ulmoides Oliver (Eucommia ulmoides Oliver), known as "man elm" in ancient times, also known as "kapok", belongs to the deciduous tree of Eucommiaceae. It is a unique economic tree species in my country. It has a cultivation history of more than 2,000 years. above. The dry bark of the Eucommia plant has many functions such as nourishing the liver and kidney, strengthening bones and muscles, lowering blood pressure, anti-tumor, and antibacterial. However, the bark of Eucommia generally needs to grow for 15 to 20 years, which is a long cycle, relatively scarce and expensive resources. The resources of Eucommia leaves are relatively rich and easy to obtain. There are about 280,000 hectares of Eucommia forests in the country...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07D311/30C07D311/40
Inventor 邵平洪台孙培龙刘青梅
Owner ZHEJIANG UNIV OF TECH
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