Method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation
A kind of technology of beautiful chicken blood vine and cell suspension, applied in the fields of biochemical equipment and methods, plant cells, tissue culture, etc., can solve problems such as difficulty in guaranteeing the yield and quality of polysaccharide in cattle, impact on yield and quality, and occupation of large arable land, etc. Achieve the effect of large development and utilization value, consistent growth cycle, and no occupation of cultivated land
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Embodiment 1
[0041] Acquisition of non-embryogenic single-cell suspension cell line of Spathifolia and production of crude polysaccharide from Spathiphyllum non-embryogenic single-cell suspension culture cells
[0042] (1) Acquisition of the non-embryogenic single-cell suspension cell line of Spathiphyllum Spatholobus
[0043] (1) Take the young stems of Spatholobus sagittatus as explants. The young stems are 1-2cm in length and contain 1-2 axillary buds. base + sucrose 30g / L+6-BA3mg / L+NAA0.5mg / L+0.8g / L agar, pH5.85), cultured at 27℃, with a light intensity of 3000Lux and a daily light duration of 12 hours. After 30 days, non- Embryogenic callus, the diameter of non-embryogenic callus is about 0.5cm;
[0044] (2) Inoculate the callus with a diameter of about 0.5cm (non-embryogenic callus is 0.125-0.25cm) 3 ,) non-embryogenic callus was transferred to callus proliferation medium (MS medium+sucrose 30g / L+6-BA4mg / L+Picloram0.5mg / L+0.8g / L agar, pH5.85), 27 Cultivate in dark at ℃, and the cu...
Embodiment 2
[0051] Identification of the main chemical constituents of the suspension culture cells of Spatholobus palisana
[0052] (1) TLC identification: take 1L of suspension cells of Spatholobus palisana and 100g of dried bovine herb (as a control), add distilled water to 1L, heat at 100°C for 1 hour, filter and save the extract, add 1L of distilled water to the residue, and repeat The above steps were performed once, and the extracts were combined. Take 5 μL of the test solution of Spatholobus palisana and the extract of Niu Dali, and load the sample on the same 5cmх4cm Merck SG60F 245 On the prefabricated plate, develop with chloroform-methanol (volume ratio 6:1), use 5-10% concentrated sulfuric acid ethanol solution as color developer, and heat to develop color. The results showed that the bands of the test sample and the extract (standard) obtained from Herba Dali herb were consistent ( figure 2 ), indicating that the ingredients of the two are the same, and the main ingredien...
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