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A kind of method for inducing the cluster sprouts of water radish

A technology of water shield and axillary buds, applied in the field of plant tissue culture, can solve the problems of limited provenance, degradation of water shield seed, quality and yield decline, etc., and achieve the effects of high yield, easy method and convenient operation

Active Publication Date: 2019-04-05
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the lack of effective variety purification and rejuvenation and variety protection for many years, the seed property of water shield has been seriously degraded, and the quality and yield are declining.
[0004] At present, there is still a lack of mature technology that can multiply high-quality engineering seedlings of water shield on a large scale, and at the same time overcome the problem of limited provenance and gradual degeneration of this species in the natural environment

Method used

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  • A kind of method for inducing the cluster sprouts of water radish
  • A kind of method for inducing the cluster sprouts of water radish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A, explant selection and pretreatment: select the water shield stem section that can germinate axillary buds with bright green color and less silt coverage as explants, soak 10min with alkaline detergent, and gently brush off the surface silt with a soft brush until The stem segment is green, rinsed with tap water for 1 hour, repeatedly rinsed with distilled water for 4-5 times, and then repeatedly washed with sterile water for 4-5 times, and set aside;

[0044] B. Sterilization of explants: Soak in 75% ethanol for 30 seconds on the aseptic operating table, rinse 4-5 times with sterile water, and then use 0.1% mercuric chloride (add a few drops of Tween 80) to clean the surface Disinfect for 10 minutes, rinse with sterile water 4-5 times, blot the water dry on sterile filter paper, and set aside;

[0045] C. Bud induction: select MS solid medium with 1.0 mg / L 6-BA and 0.15 mg / L NAA, the mass concentration of sucrose is 3%, the mass concentration of activated carbon is 0...

Embodiment 2

[0050] A, explant selection and pretreatment: select the water shield stem section that can germinate axillary buds with bright green color and less silt coverage as explants, soak 10min with alkaline detergent, and gently brush off the surface silt with a soft brush until The stem segment is green, rinsed with tap water for 1 hour, repeatedly rinsed with distilled water for 4-5 times, and then repeatedly washed with sterile water for 4-5 times, and set aside;

[0051] B. Sterilization of explants: Soak in 75% ethanol for 30 seconds on the aseptic operating table, rinse 4-5 times with sterile water, and then use 0.1% mercuric chloride (add a few drops of Tween 80) to clean the surface Disinfect for 10 minutes, rinse with sterile water 4-5 times, blot the water dry on sterile filter paper, and set aside;

[0052]C. Bud induction: select MS solid medium with 1.0 mg / L 6-BA and 0.15 mg / L NAA, the mass concentration of sucrose is 3%, the mass concentration of activated carbon is 0....

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Abstract

The invention discloses an inducing method of cluster buds of brasenia schreberi and belongs to the field of tissue culture of brasenia schreberi. The inducing method comprises the steps as follows: selection and pretreatment of explants, sterilization of the explants, induction of buds and multiplication culture of the buds. Components of a culture medium comprise macroelements, microelements, ferric salt, a complexing agent, an organic component, an inorganic additive, a plant growth regulator, a physiological activator, a carbon source, a coagulator and other additives. With the adoption of the culture medium and the culture method for induction of the cluster buds of the brasenia schreberi, the induction rate of the buds of the brasenia schreberi can be effectively increased, the bud differentiation rate is high, a large number of high-quality test-tube plantlets can be formed later in a short term, and seedlings are provided for culture of special aquatic vegetables in China. The inducing method can be used for breeding of the brasenia schreberi and can also be applied to rapid in-vitro propagation and germplasm preservation of the brasenia schreberi and other fields.

Description

technical field [0001] The invention provides a method for inducing clustered buds of water shield, belonging to the technical field of plant tissue culture. Background technique [0002] Water shield, a kind of aquatic plant belonging to Nymphaeaceae, is included in China's national first-class key protected wild plants (approved by the State Council on August 4, 1999). It is a precious wild aquatic vegetable that contains acidic polysaccharides, proteins, amino acids, vitamins, and histamine. And trace elements, etc., its value is mainly reflected in medical value and food value. Water shield is rich in glial protein, carbohydrates, fat, multivitamins and minerals. Commonly eaten water shield has health effects of both medicine and food, and is one of the precious aquatic vegetables. [0003] However, due to the lack of effective variety purification and rejuvenation and variety protection for many years, the seed property of water shield has been seriously degraded, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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