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Method for producing selenized dendrobium huoshanense culture

A technology of Dendrobium Huoshanense and its production method, which is applied in the field of production of selenized Dendrobium Huoshanense cultures, can solve the problems of slow growth of test-tube seedlings, high disease or death rate, and difficulty in ensuring photosynthetic physiological activities, etc., to improve industrial competitiveness , Increase economic benefits, high product quality effect

Active Publication Date: 2012-12-26
安徽省华信生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the particularity of the growth environment and physiological traits, when the test-tube plantlets are removed from a sterile culture container with stable light, temperature, and humidity for planting, normal photosynthetic physiological activities are difficult to be guaranteed, and the growth and development of test-tube plantlets are delayed, diseased or dead. Therefore, in the stage of transplanting and domestication of test-tube seedlings, it is necessary to add hormones to enhance the photoautotrophic ability, stress resistance and transplanting survival rate of test-tube seedlings; Pesticides to control pests and diseases

Method used

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  • Method for producing selenized dendrobium huoshanense culture

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Experimental program
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Effect test

Embodiment 1

[0023] A kind of concrete production operation step of selenized Dendrobium Huoshanense culture is as follows:

[0024] Step 1, induction of aseptic seedlings of Dendrobium Huoshanense: take ripe Dendrobium Huoshanense fruit, rinse it with an alcoholic aqueous solution of 70mL / 100mL for 5 minutes, then soak it in an aqueous solution of sodium hypochlorite with a mass fraction of 2g / 100mL for 30 minutes, and then Rinse 3 times with sterile water, remove the peel at last, take out the seeds, spread the seeds evenly on the solid high-salt medium, and cultivate them under the conditions of temperature 20±2°C, light intensity 2000±200 lux, and 12 hours of light per day for 6 month, collect the aseptic seedlings with a height of 4 to 5 cm, which are the aseptic seedlings of Dendrobium huoshanense;

[0025]The solid high-salt medium is composed of the following raw materials by weight or volume: 30000 mg sucrose, 950 mg potassium nitrate, 825 mg ammonium nitrate, 220 mg calcium chlor...

Embodiment 2

[0035] A kind of concrete production operation step of selenized Dendrobium Huoshanense culture is as follows:

[0036] Step 1, induction of aseptic seedlings of Dendrobium Huoshanense: take the mature Dendrobium Huoshanense fruit, rinse it with an aqueous alcohol solution of 75mL / 100mL for 3 minutes, then soak it in an aqueous solution of sodium hypochlorite with a mass fraction of 2g / 100mL for 20 minutes, and then Rinse 5 times with sterile water, remove the peel at last, take out the seeds, spread the seeds evenly on the solid high-salt medium, and cultivate them under the conditions of temperature 20±2°C, light intensity 2000±200 lux, and 12 hours of light per day for 6 years. month, collect the aseptic seedlings with a height of 4 to 5 cm, which are the aseptic seedlings of Dendrobium huoshanense;

[0037] The solid high-salt medium agar is 5500 mg, and the weight or volume of other raw materials is the same as in Example 1;

[0038] Step 2. Breeding of selenium-tolerant...

Embodiment 3

[0043] A kind of concrete production operation step of selenized Dendrobium Huoshanense culture is as follows:

[0044] Step 1, induction of aseptic seedlings of Dendrobium huoshanense: take the ripe fruit of Dendrobium huoshanense, rinse it for 4 minutes with an aqueous alcohol solution with a volume fraction of 75mL / 100mL, then soak it with an aqueous sodium hypochlorite solution with a mass fraction of 2g / 100mL for 25 minutes, and then Rinse 4 times with sterile water, finally remove the peel, take out the seeds, spread the seeds evenly on the solid high-salt medium, and cultivate them under the conditions of temperature 20±2°C, light intensity 2000±200 lux, and 12 hours of light per day for 5 days. month, collect the aseptic seedlings with a height of 4 to 5 cm, which are the aseptic seedlings of Dendrobium huoshanense;

[0045] The solid high-salt medium agar is 6000 mg, and the weight or volume of other raw materials is the same as in Example 1;

[0046] Step 2. Breedin...

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Abstract

The invention discloses a selenized dendrobium huoshanense culture and a method for producing the selenized dendrobium huoshanense culture. The method comprises the steps of: inducing to obtain an aseptic seedling of dendrobium huoshanense, breeding to obtain callus of selenium-resistant dendrobium huoshanense by collaboration of dark and low-salt medium, domesticating the selenized dendrobium huoshanense callus by the low-salt medium assisted by blue light, and producing the selenized dendrobium huoshanense culture by illumination of a fluorescent lamp. The mass fraction of organic selenium in dried product of the selenized dendrobium huoshanense culture produced by the method described by the invention is not less than 50mug / g. According to the method provided by the invention, high-quality dendrobium huoshanense substitute without hormone and pesticide residue can be produced at low cost and large scale via standardization; the selenized dendrobium huoshanense culture product has activity quality of the dendrobium huoshanense and the organic selenium, and can be applied to medicines and health-care products.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a production method of a selenized Dendrobium Huoshanense culture. Background technique [0002] Selenium is one of the essential trace elements in human life activities. It has obvious physiological functions such as delaying aging, improving immunity, anti-cancer, anti-oxidation, anti-radiation, and anti-plant diseases. Selenium is seriously lacking in most parts of my country, and most of the earth's surface is selenium-poor soil and water. Most of the selenium content in human food cannot meet the needs of the human body for selenium. Therefore, developing selenium-enriched products and increasing selenium intake are important for improving people The health level of the masses is of great significance. [0003] Huoshan Dendrobium ( Dendrobium huoshanense ) is a herbaceous plant of the genus Dendrobium in the family Orchidaceae. It has the effects of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨思林
Owner 安徽省华信生物药业股份有限公司
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