Probe for detecting matrilinear inheritance chondriosome deafness gene A1555G and its use

Abstract

The invention designs two pieces of Taqman mutant type and wild type probe and a pair of primer. Using Taqman probe method of real time fluorescence quantitation carries out genotype analysis for A1555G mutation of deaf gene of maternal inheritance mitochondria so as to diagnose genetic deaf disease of maternal mitochondria. The method is suitable to large-scale screening or preventative inspecting A1555G mutation of deaf mitochondria gene of maternal inheritance. Features are: simple, time saving, high specificity, high sensitivity, intuitive tested result, accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, specifically a probe for detecting maternal mitochondrial genetic deafness and its use, more specifically a real-time fluorescent quantitative probe for detecting the mutation of maternal mitochondrial deafness gene A1555G and its use. The present invention also relates to the application of the probe and its related products in the preparation of kits or similar products for diagnosing maternally inherited mitochondrial deafness. Background technique [0002] Aminoglycoside antibiotics (streptomycin, gentamycin, kanamycin, tobramycin and micronomycin, etc.) are widely used clinically because of their broad-spectrum and high-efficiency antibacterial effects and low prices. Controls Gram-negative and positive bacterial infections, but these antibiotics have serious ototoxic side effects that can cause irreversible hearing loss in patients. In the past ten years, studies have found tha...

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Problems solved by technology

In foreign countries, restriction enzyme BsmAI (Alw26I) digestion combined with sequence analysis is widely used to identify the A1555G mutation of mtDNA. The enzyme digestion identification is convenient and fast, and the results are reliable. Compared with sex enzymes, the price difference is 50-100 times; at present, the mitochondrial gene A1555G mutation detection kit (patent number: ZL03156762.2) is mainly used in China. A new restriction enzyme site, and then digested with Hae III restriction enzyme (Dai Pu et al., Chinese Hearing and Speech Rehabilitation Science, 2004, 6: 21-23), although it has a lower cost than BsmAI (Alw26I) However, it still needs cumbersome processing after polymerase chain reaction, which takes a long time. To overcome this shortcoming, we designed a simpler, faster, more accurate, less polluting and lower-cost detection method to meet the The need for extensive screening for the A1555G mutation in mtDNA

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