Method for culturing golden larch callus by taking phyllopodium as explant
A technology of callus and culture method, which is applied in the field of tissue culture of pine plants, can solve problems such as the shortage of pine chinensis medicinal resources, achieve the effects of solving the shortage of plant resources and medicinal material resources, reducing production costs, and shortening the growth cycle
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Embodiment 1
[0014] (1) Selection and disinfection of materials: choose the buds on the pine plants that grow healthy after falling leaves and have no damage by diseases and insect pests, put the buds on the ultra-clean workbench after peeling off the bud scales on the buds, and use a concentration of 70% Disinfect with alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 15 minutes, and finally rinse with sterile water for 4 times and dry the water with sterilized filter paper;
[0015] (2) Induction culture of callus: Take the sterilized shoots, cut the leaf primordia on the shoots as explants and insert them into the callus induction medium MS+6-BA 1.0mg L -1 +NAA 0.3mg·L -1 +IAA 0.1mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 The culture was carried out in medium, the pH value was 6.0, the culture temperature was 25°C, the light was 12 hours a day, and the light intensity was 1500lx. After 40 days of culture, the callus induction rate was 98%;
[0016] (3) Prolifera...
Embodiment 2
[0018] Change the callus induction medium in step (2) of Example 1 to MS+6-BA 2.0mg L -1 +NAA 0.5mg·L -1 +IAA0.3mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 , other steps were the same as in Example 1, and the callus induction rate was 85% after the explants were cultured for 40 days.
Embodiment 3
[0020] Change the callus proliferation medium in step (3) of Example 1 to MS+6-BA 1.0 mg L -1 +IAA 0.5mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 , the other steps were the same as in Example 1, and the callus proliferated 1.8 times after culturing for 40 days.
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