In vitro tissue cultivation method of potted anthurium andraeanum varieties

Abstract

The invention discloses an in vitro tissue cultivation method of potted anthurium andraeanum varieties. A leaf blade, a leaf stem, a flower head, a spathe blade or a lateral bud is taken as an explant material; after the explant material is sterilized, the explant material is inoculated to an appropriate culture medium and cultivated for 40-60 days, and then dedifferentiates to form a callus, and the callus rate can reach 87.5%; the material is continuously cultivated for 40-60 days and differentiates again to form an adventive bud, and the differentiation rate can reach 94.12%; and a large quantity of tube seedlings can be rapidly cultivated through multiplication, when the tube seedlings grow to certain heights, the tube seedlings are transferred into the culture medium and transplanted after the tube seedlings take root, and the survival rate can be larger than 95%. With the adoption of an in vitro tissue cultivation technology, a regeneration plant can be stably and efficiently obtained through the way that the callus induces the adventive bud, and rapid and mass propagation of the potted anthurium andraeanum varieties can be achieved; and according to the characteristics of different anthurium andraeanum varieties, the appropriate explant material and a culture medium condition are selected, and the method has a high application value in the production of seedlings of excellent anthurium andraeanum varieties.

Description

technical field [0001] The invention relates to a method for in vitro tissue culture of anthurium potted plant species explants. Background technique [0002] Anthurium ( Anthurium andraeanum ) is a perennial herbaceous flower of the genus Anthurium in the Araceae family. It is native to the tropical rain forests of South America and likes high temperature and high humidity environmental conditions. Anthurium has shortened stems, aerial roots on nodes, plant height 50-80cm, leaves gathered on top of stems, leathery, heart-shaped, oval, etc. Flowers and leaves grow in turn, a branch of flower emerges from one leaf, spathe is heart-shaped and oval, and the colors are red, pink, white, green, etc. The spadix is ​​sessile and yellow, and can bloom all year round under suitable growth conditions . Because of its unique flowers and leaves, the flowering period lasts for several months, so it is widely used in indoor and outdoor flower decoration. [0003] Anthurium has a long...

AI Technical Summary

Problems solved by technology

[0003] Anthurium has a long growth cycle, and conventional seed propagation and branch propagation are difficult to meet the needs of the flower market. Plant tissue culture technology is undoubtedly an effective way to shorten the propagation cycle and supply a large number of seedlings, and can ensure the excellent variety characteristics of the female parent.

Since the 1970s, foreign Pierk and others successfully established anthurium tissue culture system by using leaf explants, the tissue culture propagation method has gradually replaced the traditional propagation method. characteristics, loved by the Chinese people, the domestic market demand for anthurium seedlings is increasing day by day, domestic researches on its tissue culture regeneration technology have been carried out, and some progress has been made, but there are still some problems, such as explant selection, There is no relatively unified statement on treatment methods, medium composition, hormone conditions, etc., and the conclusions are diverse, which is related to the different experimental varieties selected by the researchers, and the conclusions are different due to the differences between varieties.