50 results about "Monocotyle" patented technology

This invention concerns a method of making an anti-infective composition for oral administration using an agent selected from the group consisting of lactoferrin protein and lysozyme protein. The method comprises three steps. The nucleic acid, which encodes a lactoferrin or lysozyme protein, is expressed in a monocot seed. The protein is isolated from the seed. This protein isolation compromises greater than 3% of the total soluble protein in the monocot seed. The protein is then formulated for oral administration.

The invention relates to application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice. Nucleotide sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.1. Encoded protein sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.2. Conditions that are met for the nucleotide sequences and the encoded protein sequences include: first, DNA (deoxyribonucleic acid) sequences shown on the 88th site to the 1005th site in the sequence table SEQ ID NO.1, or the sequences highly homologous to the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1; second, other encodable sequencecs with the same protein as the DNA sequences shown in the sequence table SEQ ID NO.2; third, the sequences the same functional as the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1, or sub-segments contained in the highly homologous DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1. The Arabidopsis transcription factor is applied to breeding of the drought-resistant salt-tolerant rice. An expression vector of the MYB44 gene can be introduced into plant cells by biotechnology. Transforming hosts, available to use the expression vector containing the MYB44 gene, can be monocotyledons such as rice, corn and wheat. The Arabidopsis transcription factor is also applicable to dicotyledons such as tobacco and soybean. The Arabidopsis transcription factor is used to breed drought-resistant salt-tolerant plant varieties.

The invention provides an artificial breeding method of a locust. The method is characterized in that geographical place is selected, wherein the place has sufficient sunlight, good drainage, and is in peace, in east-west direction, and far away from a cotton field, an orchard and other places to which pesticides are generally applied, so as to prevent locust from poisoning and death as well as facilitate water drainage in the rainy season. The breeding method is easy to master, simple to manage, free of limitation from area, suitable for all people to breed, and low in requirements on operators; the feed in the method includes maize, wheat, corn seedling and some monocotyledons, so that the feed is low in price, and the breeding cost is low; the locust reproduction rate is high, the reproduction cycle is short, and the economic benefit is high; the building adopted in the method is a greenhouse which is simple to construct and low in cost; proper place benefits the breeding; in addition, a rainproof layer covers the outer side of the greenhouse, so that the locust can be effectively prevented from being influenced by natural rainwater, and the production and survival rate can be ensured.

The invention discloses an artificial synthesized Bt insecticidal gene for transgenic anti-insect plants, further, the invention relates to the optimization and transformation systematically to a coding frame and a codon of a CrylAc anti-insect gene, according to an activity analysis to Bt protein in different regions and in accordance with the coding characteristics of the monocotyledons, obtaining anti-insect gene CrylAc-M which can be expressed in a monocotyledons efficiently, with the optimized codon and coding frame. The CrylAc-M gene after being transformed is connected on an expression carrier with CaMV35S-Adhl as promoter, and then the Bt gene CrylAc-Adhl is transferred into a maize inbred line with highly efficient conversion rate by a gene gun co-transformation method. A transgenic progeny is positive through Bt protein testing and a field anti-insect test of the field is tested to be highly resistant.

Methods for producing corn with increased transformability are provided. Markers for increased transformability are provided as well as their use to obtain corn plants with increased transformability. Locations on chromosomes that effect transformation efficiency of monocots are identified.

The present invention discloses that, formic acid or salt thereof, is an effective pre-emergent and post-emergent herbicide. Formic acid biodegrades to carbon dioxide and water thus posing not threat the environment. With proper formulation and the use of respiratory protection, formic acid also poses no threat to the applicator. Formic acid is demonstrated in this invention to control both monocotyledonous and dicotyledonous plants.

A method of making biopreform from the stem of monocotyledonous caudex plant, that is suitable for liquid infiltration and gaseous transportation of materials, is disclosed. Wood from caudex stem of trees such as coconut (Cocos nucifera), palmyra palm (Borassus flabellifer), date palm (Phoenics dactylifera), is used as a precursor material which is transformed under simple pyrolitic conditions under self-generated ambient atmosphere to biopreform having microstructural features typical of a monocotyledonous caudex tree. The biopreform is capable of liquid infiltration and gaseous transportation processing of materials in an appreciably shorter processing periods, because of its preservation of the structural and anatomical features of the parent plants with high precision.

The invention provides an anti-insect protein Cry1A.401, which is characterized by comprising: (1) an amino acid sequence shown as SEQ ID No.2, or (2) an amino acid sequence which is obtained by performing substitution, deletion and / or addition of one or more amino acids on the amino acid sequence shown as SEQ ID No.2 and has equal function. As proved by an in-vitro experiment, a modified and synthesized Bt gene toxoggenic protein has a remarkable insecticidal effect on corn borers. The anti-insect protein Cry1A.401 can be stably and efficiently expressed in monocotyledons, and is further applied to production of anti-insect transgenic plants.

The invention discloses an endogenous promoter WY7 of powdery mildew of a rubber tree and application of the endogenous promoter WY7. The promoter WY7 has a nucleotide sequence as shown in the SEQ IDNO:1, or the promoter has a variant having the function of the promoter. The invention further relates to a nucleic acid construction body comprising the promoter, a carrier, a reconstitution cell, atransgenic plant, an explant and a callus. The promoter is used for regulating the expression of exogenous target genes in the dicotyledon and the monocotyledon, and provides a brand new tool and choice for the expression of genes of the transgenic plant.

The invention provides a new compound, namely N,N-diethyl-2-(2-methoxy-5-nitrophenoxyl)-ethylamine. The N,N-diethyl-2-(2-methoxy-5-nitrophenoxyl)-ethylamine has an amino ether skeleton and can regulate the growth of plants. The compound is used for performing biological activity experiment on monocotyledon wheat, dicotyledon mung beans and peanuts. The experiment result indicates that for the mungbeans, the peanuts and the wheat, the compound has a remarkable acceleration effect when the concentration is low and has a certain inhibition effect when the concentration is high.

A granulated organic and organomineral fertilizer supplemented with biological additive and process for the production of granulated organic and organomineral fertilizer supplemented with biological additive is physically presented in the form of granules (G), having its formulation defined by the combination of organic and mineral matter (N,P,K), to which it is added, in addition, a bioburden in the form of bacteria, fungus and yeast that are selected to crops of interest, whether grasses, legumes, monocotyledons and dicotyledons, the granules (G) receive the bioburden at the time of its granulation; the selection of bioburden to be included in granules (G) is established according to the soil and crop analysis to be cultivated.

The invention provides an inducible promoter containing a W box as well as a construction method and the application to genetic engineering. The inducible promoter at least contains a nucleotide sequence shown as SEQ ID No.1. The construction method comprises the following step of: fusing a stress-response-stressed W box identified in dicotyledon arabidopsis thaliana with a CaMV35S promoter core sequence to construct an inducible promoter. The inducible promoter containing the W box in the invention can strongly response the invasion of rice blast fungus and the feeding of rice cnaphalocrocis medinalis in transgenic rice, has obvious response activity on the treatment of small-molecule compounds with disease resistance after separation identification and has poor response activity on abiotic stress treatment, such as drought, low temperature, high temperature, and the like. The inducible promoter has very important application values in the disease-resistant and pest-resistant genetic engineering of monocotyledon and dicotyledon, and transgenic plant strains also have very important application values in the high throughput screening of small-molecule compounds for inducing plants to resist diseases.

The invention belongs to the technical field of proteomic technology, and particularly discloses an extracting method for phosphorylated protein suitable for two-dimensional electrophoresis of plasmamembranes of rice leaves for the first time. The extracting method comprises the steps of extraction for coarse plasma membranes of the rice leaves, purification of the plasma membranes of the rice leaves, enrichment of the phosphorylated protein of the plasma membranes of the rice leaves and the like. The extracting method is simple in operation, high in extracting efficiency and low in cost, thepurity of the plasma membranes of the rice leaves can reach 93% or above, and the yield of the enriched phosphorylated protein of the plasma membranes from protein of the plasma membranes is 3.33-3.89%. The extracting method is suitable for the plant materials with difficultly extracted phosphorylated protein of the plasma membranes and fewer interferents; the obtained phosphorylated protein of the plasma membranes contains fewer impurities and interferents and can meet the requirements of two-dimensional electrophoresis, the obtained two-dimensional electrophoretogram is clear in background,uniform in protein spot distribution and fewer in longitudinal trailing and horizontal transverse grain phenomenon and has good repeatability, and the extracting method is suitable for preparation and analysis of phosphorylated proteomes of the plasma membranes of monocotyledons.

The invention discloses an amide compound. The amide compound has the following chemical structural formula. The structural formula is as shown in the specification. The preparation method comprises the following steps: reacting 2,4-dichlorbenzoxy acetyl chloride and urea to obtain the amide compound. The amide compound has excellent herbicidal activity, particularly the herbicidal activity on dicotyledon weeds is obviously superior to that of monocotyledon weeds. The preparation method of the amide compound is simple in operation and high in yield.

The present invention provides methods and compositions for making and using transgenic plants that exhibit increased nitrogen storage capacity compared to wild-type plants. Methods of the invention comprise inducing overexpression of monocot-derived vegetative storage proteins (VSPs) in plants, particularly in monocots. In some embodiments, at least one nucleotide construct comprising a nucleotide sequence encoding the ZmLox6 protein or a biologically active fragment or variant thereof is introduced into a plant. Depending upon the objective, the nucleotide construct may optionally comprise an operably linked coding sequence for a vacuolar sorting signal or plastid transit peptide in order to direct storage of the ZmLox6 protein or biologically active fragment or variant thereof into the vacuolar compartment or plastid compartment, respectively, of the cells in which the VSP is expressed. The invention further provides methods for producing plants with increased nitrogen content and / or increased nutritional value, which is desirable in commercial crops, including those used for forage, silage, and grain production.

The present invention provides a method for modifying a targeted site in double-stranded DNA possessed by monocot plant cells, which includes a step for, by bringing a complex, in which a nucleic acidsequence-recognizing module that specifically bonds with a target nucleotide sequence in a selected double-stranded DNA is bonded with a nucleic acid base converting enzyme, into contact with the double-stranded DNA, converting one or more nucleotides at the targeted site to one or more different nucleotides, deleting one or more nucleotides at the targeted site, or inserting one or more nucleotides to the targeted site, without cleaving at least one of the strands of the double-stranded DNA at the targeted site, wherein the contact between the double-stranded DNA and the complex is achievedby introducing a nucleic acid coding for the complex to the monocot plant cells. In addition, also provided is the complex to be used in this method, in which the nucleic acid sequence-recognizing module that specifically bonds with a target nucleotide sequence in a double-stranded DNA possessed by monocot plant cells is bonded with a nucleic acid base converting enzyme.

The invention discloses an application of a SCWBC11 protein in culture of a male sterility line of rice, and provides an application of a substance which is expressed by an OSWBC11 protein coding gene in a silent or inactivated target plant to culture of a male sterile plant. In the application, the amino acid sequence of the OSWBC11 protein is the sequence 2 in the sequence table. The target plant is dicotyledon or monocotyledon and monocotyledon is specifically rice. Experiments verify that by adopting an RNA (Ribonucleic Acid) interference method, OSWBC11 protein expression interference is carried out on wild rice Nipponbare, so that the protein function is lost and rice cannot produce pollen, thereby forming the male sterility line. Therefore, the protein has an important application value to the forming mechanism of pollen of rice and the application of the protein to crossbreeding of rice can be explored.

The present invention relates to a method for targeted inactivation of transcription factor using an artificial small interfering peptide and a use thereof. According to the present invention, an artificial small interfering peptide (a-siPEP) as a truncated from of the transcription factor for regulating transcription by dimerization was produced. It was also confirmed that, as a-siPEP forms a heterodimer with a transcription factor, DNA binding and transport into a nucleus of the transcription factor are inhibited, so that inactivation of the transcription factor is achieved at protein level. The method for inhibiting transcription factor activity using a-siPEP can replace a gene knock-out method and it allows protein-level inhibition of a transcription factor. Also, it is a transcription regulation method with high precision and high efficiency that can be applied for both monocot and dicot plants.

The present disclosure relates, according to some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant (e.g., a monocot) using a promoter operable in one or more plant tissues and / or cells. In some embodiments, an artificial nucleic acid may comprise an expression control sequence having the sequence selected from the sequence of nucleotides 1-4710 of SEQ ID NO: 1 and the sequence of nucleotides 1137-4710 of SEQ ID NO: 1, wherein the expression control sequence has stem-regulated promoter activity in at least one monocot (e.g., at least two monocots).

The present invention provides methods and compositions for making and using transgenic plants that exhibit increased nitrogen storage capacity compared to wild-type plants. Methods of the invention comprise inducing overexpression of monocot-derived vegetative storage proteins (VSPs) in plants, particularly in monocots. In some embodiments, at least one nucleotide construct comprising a nucleotide sequence encoding the ZmLox6 protein or a biologically active fragment or variant thereof is introduced into a plant. Depending upon the objective, the nucleotide construct may optionally comprise an operably linked coding sequence for a vacuolar sorting signal or plastid transit peptide in order to direct storage of the ZmLox6 protein or biologically active fragment or variant thereof into the vacuolar compartment or plastid compartment, respectively, of the cells in which the VSP is expressed. The invention further provides methods for producing plants with increased nitrogen content and / or increased nutritional value, which is desirable in commercial crops, including those used for forage, silage, and grain production.

A method of making biopreform from the stem of monocotyledonous caudex plant, that is suitable for liquid infiltration and gaseous transportation of materials, is disclosed. Wood from caudex stem of trees such as coconut (Cocos nucifera), palmyra palm (Borassus flabellifer), date palm (Phoenics dactylifera), is used as a precursor material which is transformed under simple pyrolitic conditions under self-generated ambient atmosphere to biopreform having microstructural features typical of a monocotyledonous caudex tree. The biopreform is capable of liquid infiltration and gaseous transportation processing of materials in an appreciably shorter processing periods, because of its preservation of the structural and anatomical features of the parent plants with high precision.

The invention discloses a corn drought induced expression gene ZmRD101 promoter and application thereof. The nucleotide sequence of the corn drought induced expression gene ZmRD101 promoter is shown as SEQ ID NO. 1. The promoter of the gene ZmRD101 has the characteristics of induced high expression under drought and low expression in normal growth, and can be used in the field of transgenic breeding. A plant expression vector containing the promoter is constructed and transformed into a genome of a plant such as monocotyledon corn, and a target gene is driven to be specifically and highly expressed under drought and to be low expressed under a normal state. The strain can be used for balancing the contradiction between yield and stress resistance, is used for cultivating a new breeding material, and provides a basis for further stress-resistant breeding of corn.

The invention discloses application of rice spliceosomes GS3.2 in regulation and control of rice grain traits. The invention identifies that a GS3 gene has a plurality of spliceosomes GS3.1-GS3.6, wherein the main spliceosomes are GS3.1 and GS3.2, which respectively account for about 50% and 40% of a total transcript, while GS3.2 reduces the level of GS3.1 form at the RNA level on the one hand, and competitively inhibits signal transduction of GS3.1 at the protein level on the other hand, thereby inhibiting the negative regulation and control effect of GS3.1 and achieving the effect of maintaining the normal size of rice grains. On that basis, the invention inhibits the negative grain size regulation and control function of GS3.1 by expressing GS3.2 in a GS3.1 high-expression strain, thereby achieving the purpose of maintaining the normal size of rice grains. The invention not only solves the problem that the functional differentiation mechanism of the dicotyledon AGG3 and the monocotyledon GS3 is still unclear, but also can be applied to the fields of actual production, breeding and the like of rice as a new rice grain trait regulation and control means.

The present invention relates to nucleic acid molecules found in the genome of the Citrus Leprosis Virus (CiLV), which is associated to Citrus Leprosis (CiL) disease. The cloned CiLV nucleic acid molecules can be used as probes or can be used to design oligonucleotide primers useful in assays, such as a polymerase chain reaction, for detecting the presence of CiLV in biological samples, particularly leaves, roots and other tissues or organs of plants, such as plants from the genera Citrus and Poncirus. The invention comprises introducing the mentioned nucleic acid molecules in cloning vectors and cloning the recombinant nucleic acid molecules in cells, such as prokaryotes (e.g., bacteria like E. coli), and eukaryotes (e.g., yeast, COS, CHO, and other cells). The cloned CiLV nucleic acid molecules are expressed in cells to provide immunogenic proteins which can be used to raise antibodies against the CiLV, which can then be used to detect the presence of the CiLV virus in biological samples. It also comprises the nucleic acid molecules represented in SEQ ID Nos. 5 and 8, in whole or part, as well as transgenic plants, such as monocots and dicots, containing the CiLV nucleic acid molecules, in any kind of combination, so that expression increases resistance to CiL disease.

The invention belongs to the technical field of agrochemistry, and relates to a novel plant growth regulator 1-[2-(2-methoxy-5-nitro-phenoxy)]-ethyl 3-methylimidazole salt compound, a preparation method and use thereof. The structure of the compound is shown by the following formula: the biological activity experiments of the compound are carried out on monocotyledonous plant wheat and dicotyledonous plant soybean respectively. The results show that the compound has obvious plant growth promotion effect when the concentration is low and certain inhibition effect when the concentration is high.It is a broad-spectrum plant growth regulator. Moreover, the production process is simple and easy to realize industrial production, providing a new choice for plant growth regulators.