86 results about "Bioherbicide" patented technology

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

The present invention relates to the use of a strain of Burkholderia andropogonis for controlling the growth of a weed belonging to the order Caryophyllales. The present invention also provides a method for suppressing weed growth, comprising applying a strain of Burkholderia andropogonis to a weed belonging to the order Caryophyllales. Also provided is a biocontrol composition for controlling the growth of a weed belonging to the order Caryophyllales, the composition comprising a strain of Burkholderia andropogonis, and a suitable medium.

The invention relates to a method for preparing biological weed killer, wherein it uses Curvularia Boedijn Mds0404 CGMCC NO. 1827 as seed, which comprises 80-95% spore, 0-18% chemical weed killer, and 2-5% agent. And its production comprises that: activating seed, cultivating seed liquid, cultivating spore and collecting components. The invention has low cost and wide application.

The present invention relates to methods of using m-tyrosine compounds from Festuca species for inhibiting weed growth and enhancing growth of non-weed plants. The present invention also relates to methods of identifying plants having herbicidal properties.

The present disclosure concerns specific Pseudomonas fluorescens and Pseudomonas putida strains, specific genes and their corresponding specific gene products, the specific bioherbicide molecules whose synthesis or secretion is controlled by those genes and gene products, and methods of using these molecules to control deleterious weeds.

The invention discloses solid granules of biological weedicide Jun Kekuo and a preparation method therefor. The solid granules are prepared through granulating a solid matrix, Sclerotium rolfsii mycelial masses, a binder and water which serve as raw materials; the weight ratio of the solid matrix to the Sclerotium rolfsii mycelial masses to the binder to the water is 30: (35-55): (0.4-12): (12-16); and the solid matrix is any one or more selected from rice straws, wheat straws, rape straws, cotton stalks, Solidago canadensis straws, cane straws, soybean straws, rice hulls, cotton seed hulls, garden deadwood and wood dust, and the binder is selected from white carbon black or glutinous rice flour. According to the solid granules and the preparation method therefor, mycelia are produced by using a liquid fermentation technology, so that the time consumed is shortened to more than half of the time for solid fermentation. A solid powdered material is coated with microbial pesticide active mycelia so as to form a granular formulation, thus, the storage period is prolonged, the drug effect is improved, and the consumption is reduced.

The invention discloses a compound biological herbicide and an application method thereof, belonging to a compound application technology of a biological herbicide and an organic acid. The biological herbicide comprises used spore, mycelia and metabolite types; the organic acid is selected from an oxalic acid, an acetic acid or a formic acid; the mass ratio of the biological herbicide to the organic acid is (1:0.02)-(1:4). When the compound biological herbicide disclosed by the invention is used, the biological herbicide and the organic acid can be prepared into a water solution to be sprayed, and also can be sprayed separately. By using the compound ratio of the compound biological herbicide to the organic acid, the compatibility is good, the organic acid has a synergistic effect on the biological herbicide, and the environment-friendly effect is achieved.

The invention belongs to the technical field of organic fertilizers, and relates to an active probiotic organic fertilizer prepared by using rice bran, bean pulp and other organic matters as a culture medium and carrier by anaerobic fermentation and aerobic fermentation and application thereof. The active probiotic organic fertilizer is prepared from the following raw materials in parts by weight: 320-350 parts of soybean meal with the water content of 10-15%, 20-30 parts of rice bran with the water content of 10-15%, 380-400 parts of fish meal with the water content of 10-15%, 20-30 parts of strain, 20-30 parts of brown sugar with the water content of 3-5%, 1-2 parts of fermentation initiator and 200-210 parts of water. The organic fertilizer can be used as a straw rotting agent, a composting initiator, a garbage reducer, a sewage purifier, a biological organic fertilizer and a biological weedicide. The product is green and environment-friendly, is convenient to use, and has the advantages of wide application range, high yield, good quality and obvious use effect.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

The yard grass bipolaris crude toxins extraction process and its agnets, which belongs to the microb technology field. It includes the following steps: separate fruitbody and fermentative broth from fermentated culture of yard grass bipolaris; decompress and concentrate fruitbody abstracts and get the oil condensate; decompress, concentrate and extract fermentative broth, add some water to wash the extraction phase, then decompress and concentrate to get brown oil substance; then merge the above said substances is the total extraction of yard grass bipolaris fermented product, which is known as yard grass bipolaris crude toxins. The agent of yard grass bipolaris crude toxins contains 10-50% total extraction of yard grass bipolaris fermented product. The extraction process is very simple, the emulsion and emulsifiable formulation of total extractin agent are belongs to microbial herbicide and microbial fungicides. It can prevent ruderal and rice sheath blight. It has not residual pesticide Injury and is environment friendly.

The subject invention concerns the use of a mixture of fungal pathogens to control the growth of target weed species. Also, the invention concerns three novel fungal pathogens which, in mixture, can be used to control the growth of grassy weeds.

The present invention is culture process and use of yard grass Bipolaris sacchari, and belongs to the field of plant pathogenic fungus technology. The culture process includes the following steps: sterilizing collected infected cockspur grass sample, culturing in PDA culture medium at room temperature for 3-4 days until conidiophore of Bipolaris sacchari is observed under anatomical lens, picking out with flame sterilized dissecting needle and setting on PDA culture medium, transferring for PDA slant cultivation to form single spore line, culturing in cockspur grass seed for 10 days, packing in abacterial Eppendorf centrifuge tube and preserving at 4 deg.c; taking one cockspur grass seed with hypha and spore and inoculating to fresh PDA plate, dark culturing at 28 deg.c for 7 days, inoculating 7 mm diameter fungus block onto PDB liquid culture medium and static culturing at 28 deg.c for 14 days. Yard grass Bipolaris sacchari and its fermented liquid may be used in preparing biological herbicide and fungistat.

Biofertilizer compositions, methods for increasing nitrogen levels in soil and methods of inhibiting undesirable plant growth generally include contacting the soil with a composition comprising dried distillers solubles derived from a dry milling corn ethanol processing; and contacting the soil with the soil in amounts effective to produce the desired result, e.g., increase nitrogen levels, inhibit undesirable plant growth, and the like.

The present invention belongs to the field of application of microbe in agricultural plant protection. The biological herbicide is batch produced with fungus strain psorosperm anthrax bacteria QZ-97a, purplesteem eupatorium hypnocyst, crab grass hyperbolic spora, common zinnia hypnocyst, etc. and through combined liquid and solid fermentation. During the liquid and solid fermentation, the agricultural product leftover is used as culture medium material, and proper initial temperature is benefit for thallus to obtain nutritive matter and oxygen transmitted and to obtain high conversion rate. Solid fermentation has wider temperature range and easy-to-obtain lighting condition. The process is simple and easy to operate.

Methods and compositions for environmentally-friendly weed control are provided. In particular, bioherbicide compositions are provided comprising plant extracts selected from Russian Olive, Austrian Pine, Tree of Heaven, Autumn Olive, and Black Walnut extract, either alone, or combination with at least one mulching substrate. Methods of using the herbicide compositions are also provided.

The invention relates to an allelopathic bacteria-enterobacter agglomerans W5 strain and application thereof as a bioherbicide, belonging to the field of microbes applied to agricultural plant protection. The allelopathic bacteria-enterobacter agglomerans W5 strain is preserved in the China general microbiological culture collection center (address: DaTun road, Chaoyang district, Beijing), with a preserving number of CGMCC No. 4565. The invention also relates to a liquid weeding bacterial agent and a solid weeding bacterial agent containing the allelopathic bacteria-enterobacter agglomerans W5 strain as effective components and applications thereof in controlling dicotyledonous weeds of a farmland or a lawn.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site. The method comprises the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type hydroxyphenyl pyruvate dioxygenase or a mutated hydroxyphenyl pyruvate dioxygenase (mut-HPPD) which is resistant or tolerant to a coumarone-derivative herbicide and / or a nucleotide sequence encoding a wild-type homogentisate solanesyl transferase or a mutated homogentisate solanesyl tranferase (mut-HST) which is resistant or tolerant to a coumarone derivative herbicide, and then applying an effective amount of said herbicide to said plant cultivation site. The invention further refers to plants comprising mut-HPPD and to methods of obtaining such plants.

The present disclosure concerns specific Pseudomonas fluorescens and Pseudomonas putida strains, specific genes and their corresponding specific gene products, the specific bioherbicide molecules whose synthesis or secretion is controlled by those genes and gene products, and methods of using these molecules to control deleterious weeds.

Transgenic plants resistant to bio-herbicides, particularly to phytotoxic non-protein amino acids including the meta-tyrosine (m-tyrosine) amino acid analog and salts thereof, means and methods for producing the transgenic plants.

The invention discloses a plant-sourced liquid weedicide and a preparation method thereof. With natural plant leaves as raw materials, michelia alba leaves, lilac leaves and pterocarya stenoptera leave are ground and then immersed in clear water to obtain a mixed solution of plant active components. The natural plant active components contained in the plant-sourced liquid weedicide are degraded and volatilized very easily, do not pollute the environment, are environment-friendly and have a good effect of inhibiting weed growth, and the plant-sourced liquid weedicide can be applied to both grassy weeds and broad-leaved weeds and also has a good control effect on weeds resistant to chemical weedicides such as glyphosate. Tests show that the fresh fruit mass control effects of the plant-sourced liquid weedicide on bromegrass, ryegrass and bluegrass are 90.88%, 93.23% and 91.05% respectively, the fresh mass control effects thereof on sisymbrium sophia and lithospermum arvense are 90.78% and 92.34% respectively, and namely, the plant-sourced liquid weedicide has a relatively good control effect on both the grassy weeds and the broad-leaved weeds, has the fresh mass control effects higher than 90% and can be popularized and applied as a biological weedicide on a large scale.

The invention relates to describe host-specific extracellular phytotoxins produced by Lasiodiplodia theobromae (LT, also Botryodiplodia theobromae) that has a broad range as a pre-emergent and / or post-emergent bioherbicide, this isolate has been deposited in Microbial Type Culture Collection, Chandigarh, India and given an accession number MTCC 3068, a method using LT-toxin has been developed for controlling certain herbs including Parthenium hysterophorus, duckweeds, jimsonweed, black nightshade, prickly sida and Euphorbia hirta, these phytotoxins can be used partially pure, as a cell-and spore-free filtrate, a crude filtrate, or a crude suspension of the culture and optionally along with other additives.

The invention relates to a biochemical synergistic weed killer composition. The biochemical synergistic weed killer composition comprises effective constituents, auxiliaries and fillers. The effective constituents include sclerotium rolfsii and pretilachlor. The biochemical synergistic weed killer composition has the advantages that the two effective constituents, namely, the sclerotium rolfsii and the pretilachlor, are used with each other, accordingly, the biological weed killing performance of the sclerotium rolfsii which is a novel biological weed killer can be brought into play, fast-acting properties of the pretilachlor which is a chemical weed killer can be embodied, and weed killing effects can be improved; the most obvious synergistic effects can be realized by the sclerotium rolfsii and pretilachlor composition for preventing and killing diversified broad-leaf weeds and nutgrass flatsedge of dry and direct-seeded paddy rice; the service cost of the biochemical synergistic weed killer composition can be reduced, problems of high single-dosage service costs and poor low-dosage individual service effects of the sclerotium rolfsii and the pretilachlor at present can be solved, accordingly, prevention effects can be improved when the sclerotium rolfsii and the pretilachlor are used with each other, and the agricultural cost can be reduced.

The invention discloses a biological weeding organic fertilizer. The biological weeding organic fertilizer is a solid granule prepared through wrapping a base granule with Sclerotium rolfsii Sacc. SC64 mycelia; a mass ratio of the Sclerotium rolfsii Sacc. SC64 mycelia to the base granule s 1.7-2.0:1; and the base granule is prepared from, by mass, 5-45% of an organic fertilizer, 1.3-28.6% of an adhesive, 10-15% of water, and the balance of a solid matrix. The invention also discloses a preparation method of the biological weeding organic fertilizer, and an application of the biological weeding organic fertilizer in control of dicotyledon weeds. The biological weeding organic fertilizer simultaneously has the advantages of a traditional organic fertilizer and a biological herbicide, so garden wastes and crop straws are fully used to realize changing of wastes into valuables and reduce environment pollution caused by straw burning, and the use of chemical fertilizers and pesticides is reduced to enhance the stress resistance of crops and improve the quality of the crops.

The invention belongs to the field of pesticides, and concretely relates to a botanical biological herbicide adopting a plant extract liquid as a main active component, and a preparation method thereof. The biological herbicide is prepared from, by weight, 20-40 parts of Radix Euphorbiae Ebractealatae, 35-50 parts of Common Dysosma Rhizome, 20-30 parts of Radix Stemonae, 43-50 parts of Atractylodes lancea, 2-4 parts of sodium secondary alkyl sulfonate and 1.5-1.8 parts of triethanolamine. The botanical biological herbicide adopting synergism of raw materials has a good killing effect on various weeds, and has the advantages of fast effectiveness, no untoward effects on crops, no medicine residual, improvement of the quality of crops, increase of the output of the crops, and environmental protection.

The invention discloses a bipolaris yamadae (Y.Nisik.) shoemaker and a screening and identification method and application thereof. The bipolaris yamadae (Y.Nisik.) shoemaker (HXDC-1-2) has been deposited in the China General Microbiological Culture Collection Center with the accession number CGMCC No.17778. The bipolaris yamadae (Y.Nisik.) shoemaker is applied to biological weeding when the conidia concentration is 102 conidia / ml or more, can be safely applied to crop fields of rice, corn, soybeans, peanuts, sesame, millet, broad beans, peas, rape, vegetables, flowers, traditional Chinese medicinal materials and fruit trees, and is used for controlling gramineous weeds, such as barnyard grass, semen euphorbiae, herba digitariae, herba setariae viridis, eleusine indica, leptochloa panicea,wild oat, Aleppo grass and microstegium vimineum, annular nutgrass flatsedge and part of broad-leaf weeds; and the control effect can reach 80% or more. The bipolaris yamadae (Y.Nisik.) shoemaker, serving as a biological herbicide, has the advantages of being good in weeding effect, low in usage amount, high in safety to crops, low in cost, environment-friendly, free of a residue and free of pollution.

The invention provides a biological herbicide containing spores produced by a Colletotrichum Corda strain with a collection number of CGMCC No.2867, and a method for preparing the biological herbicide and a method for using the herbicide to remove weeds from crops. The invention particularly provides a biological herbicide for removing digitaria sanguinalis from crops, which comprises the spores produced by the Colletotrichum Corda strain with the collection number of CGMCC No.2867. The biological herbicide can prevent and control weeds in dicotyledons, and prevent and control weeds in monocotyledons; and the strain particularly has 85 to 100 percent of control effect on a target weed digitaria sanguinalis, particularly on plants with less than 3 leaves.

The invention relates to a herbicide prepared by taking a lilac leaf extract as an active component as well as a preparation method and application of the herbicide. The herbicide prepared by taking the lilac leaf extract as the active component is prepared from the following components by mass percent: 70-80% of the active component and the balance of auxiliary components, wherein the auxiliary components comprise a surface active agent and filler. The components of the plant-derived herbicide provided by the invention originate from the plant bodies, and the bio-herbicide takes natural products as herbicidal active component, so that the herbicide is easily degraded and is safe and environment-friendly. The herbicide has a good prevention effect on gramineous and broad-leaved weeds, especially has a special effect for the broad-leaved weeds, and can be used as a selective herbicide for gramineous crops. Compared with the prior art, the plant-derived herbicide provided by the invention has the characteristics of being high in activity, remarkable in prevention effect, higher in selectivity, and the like. The herbicide has no harm to crops and no pollution to the environment, is free from pesticide residue, does not easily cause drug resistance, and is environmentally safe.

The invention relates to the field of biopesticides and application thereof, in particular to a biopesticide composite synergist which comprises the following raw materials in percentage by mass: 2-10% of esterified vegetable oil, 1-6% of sulfonate, 2-10% of organic silicon and the balance of water. The composite synergist can significantly reduce the contact angle and surface tension of a liquidpesticide, enhance the spreading property and adhesion of the pesticide on plant leaves, improve the permeability of the liquid pesticide, and reduce the drug resistance of pests, can be combined witha biological insecticide for disinsection, can also be combined with a biological herbicide for weeding, and can be matched with various biopesticides for use and has a wide application range.