A strain of Beauveria bassiana and its application in controlling southern blueberry grubs
A Beauveria bassiana and blueberry technology, applied in the fields of application, fungi, and microbial-based methods, can solve the impact of strain differences on the virulence of Beauveria bassiana, the difference in pathogenicity, stability and timeliness of Beauveria bassiana to pests Poor performance and other problems, to achieve good development potential and production value, to solve the effects of pesticide residues and strong toxicity
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Embodiment 1
[0016] Example 1 Preservation of Beauveria bassiana bacterial strains and strain morphological characteristics
[0017] The Beauveria bassiana strain MJ1015 is Beauveria majiangensis, which was isolated from the infested grub in the blueberry orchard of Majiang County, Guizhou Province. Committee General Microbiology Center (CGMCC), deposit number: CGMCC NO.15090, deposit address: Beijing.
[0018] Cultivated on PDA medium for 13 days, the diameter of the colony can reach 4.81mm, no odor is produced, the aerial hyphae are separated, branched, transparent, smooth, and 1.6-2.2μm wide. Sporogenous cells solitary or clustered laterally, cylindrical at base, sometimes subspherical, 3.8-12.9 (-16.2) μm long, 1.2-1.5(-2.3) μm wide, with an indistinct tooth less than 1 μm at the top axis. Conidia are oval, transparent, septate, smooth surface, 2.4-3.8 μm long, 1.6-2.3 μm wide, Q = 1.6-2.6 (L m =2.1, W m =1.4 , Q m =1.6).
Embodiment 2
[0019] Embodiment 2 The cultivation method of Beauveria bassiana and its biological characteristics
[0020] Inoculate the Beauveria bassiana strain MJ1015 in the improved PDA medium (potato 200g / L, glucose 18g / L, agar 18g / L, add distilled water to 1L, and use KH 2 PO 4 adjusted to pH 5.0), placed in climate chambers at 16°C, 19°C, 23°C, 26°C, 29°C, and 32°C (humidity is 100%), and observed and recorded mycelial growth and sporulation every 2 days quantity situation. The results showed that the most suitable temperature for bacterial colony growth and sporulation of strain MJ1015 was 26°C, and the colony diameter and sporulation yield on the 14th day at this temperature were 5.12 cm (see Table 1) and 120.45*106 spores / ml, respectively. (See Table 2).
[0021] temperature day 2 day 4 day 6 day 8 day 10 day 12 day 14 16℃ 0.09 0.25 0.28 0.67 1.07 1.41 1.73 19℃ 0.11 0.46 0.57 1.16 1.71 2.25 2.68 23℃ 0.25 0.56 0.89 2.01 2.48...
Embodiment 3
[0029] Application of Example 3 Beauveria bassiana strains in the control of blueberry pests
[0030] The bacterial strain used in the present invention is isolated from the infested grub body of the blueberry orchard in Majiang County, Guizhou Province. =4-6) The germination rate is high. It is specially used to prevent and control blueberry grub pests, especially the blueberry pests, the white star beetle, the thick ribbed beetle, and the blue flower beetle. It has the characteristics of strong toxicity and fast insecticidal speed.
[0031] The optimum soil pH range for blueberry growth is 4-6, so the acid resistance of the strain is the primary criterion for testing whether it can control blueberry grubs in the south, and it is also the key to applying the strain for field control.
[0032] The application of described Beauveria bassiana bacterial strain in the prevention and control of blueberry pests, Beetle spp.
[0033] Three strains CH1, CH2, and SWS1 (collected from...
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