399 results about "Beauveria bassiana" patented technology

A pesticidally active isolate of the fungus Beauveria bassiana, which is monosporic and which may be used in agriculture as a pesticide for the effective control of pests. The biomass of the inventive isolate contains an unusually high proportion of spores. The invention also provides methods for the preparation of the isolate and methods of formulating and using same for the effective control of crop infesting pests.

The invention relates to a white muscardine fungus granular formulation and a preparation method thereof. The white muscardine fungus granular formulation is prepared by the following compositions in mass percentage: 1 to 20 percent of white muscardine fungus spore powder, 70 to 95 percent of carrier, 0.05 to 3 percent of an adhesive, 0.5 to 5 percent of a disintegrant, 0 to 2 percent of a disintegration assistant, 0.01 to 1 percent of a dispersant, 0.5 to 5 percent of an anti-ultraviolet agent, 0 to 3 percent of a lubricating agent, and 0 to 2 percent of a coloring agent. The preparation of the white muscardine fungus granular formulation can adopt an extrusion molding granulation method, a fluidized bed granulation method and a coating granulation method. The white muscardine fungus granular formulation has the obvious characteristics that the storage period of white muscardine fungi is between 10 and 12 months, and the white muscardine fungus granular formulation can be directionally and accurately applied, is particularly applicable to killing underground pests, is convenient to pack, store, transport and use, does not pollute the environment, and can also control the release rate of the white muscardine fungi and prolong the lasting period.

The invention belongs to the technical field of environment protection, and especially relates to a composite microbial preparation for garbage deodorizing and sterilization and a preparation method thereof. The microbial preparation comprises beauveria bassiana, saccharomyces cerevisiae, lactobacillus plantar, rhodopseudomonas palustris, and bacillus thruingiensis. The comprehensive composite inoculant provided by the invention can remove the garbage odor and kill insects such as mosquito, fly, cockroach, and the like; and can be used in locations such as rubbish transfer station, digestion tank, public lavatory, landfill, etc. to remove odors and kill insects. The stinky odors given off by the garbage can be rapidly degraded under the cooperation of five microorganisms, furthermore, the bacterium proliferation is inhibited, the insects in the garbage piles such as mosquito, fly, cockroach, and the like can be killed, and the microecological environment of garbage piles can be improved, so the composite inoculant has a very important economic and environment-protection meaning.

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand/or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

The present invention relates to a new-type beauveria bassiana pesticide suspension. Its composition includes (by wt%) 1-50% of beauveria bassiana powder, 0.05-15% of chitin inhibitor, 0.25-40% of organic silicon surfactant, 0-40% of adsorbing agent, 0-20% of dispersing agent, 0-20% of moistening agent, 0-20% of antioxidant, 0-20% of photoprotection agent, 0-20% of viscosifier and 1-60% of water. Said invention also provides the concrete steps of its preparation method, and its concrete application method.

A biopesticidal composition for controlling insects (e.g., pecan weevils, the diaprepes root weevil, fall armyworm, fire ants), containing an agriculturally acceptable carrier and an effective insect (e.g., pecan weevils, the diaprepes root weevil, fall armyworm, fire ants) biopesticidal amount of a fungus selected from the group consisting of Beauveria bassiana having the identifying characteristics of Beauveria bassiana NRRL 30593, Metarhizium anisopliae having the identifying characteristics of Metarhizium anisopliae NRRL 30594, Beauveria bassiana having the identifying characteristics of Beauveria bassiana NRRL 30601, Beauveria bassiana having the identifying characteristics of Beauveria bassiana NRRL 30600, or mixtures thereof. Also, a method for controlling insects (e.g., pecan weevils, the diaprepes root weevil, fall armyworm, fire ants), involving applying an effective insect biopesticidal amount of the composition to the insects or to the plants, areas or substrates infested with the insects.

The invention relates to a preparation method and application of Beauveria bassiana, which especially has an effect on control of locust pests. The Beauveria bassiana (Bals. -Criv.)Vuill TRCC16001 provided by the invention has a registered number of CGMCC No.7997 in China General Microbiological Culture Collection Center. With a strong spore production ability, the strain has strong insecticidal activity on pygmy grasshoppers 3rd instar locust nymphae, and the lethal time of 50% LT50 is 3.04 days. The strain can be employed to effectively control the population quantity of pygmy grasshoppers. In addition, locust Metarhizium anisopliae also has very good prevention effect on other pests Dendrolimus punctatus, ostrinia nubilalis, grubs and the like, thus being worthy of popularization and application.

The invention discloses a stiff silkworm culture method. The stiff silkworm culture method comprises the following steps of a. preparing a spore suspension: taking beauveria bassiana spores to prepare the spore suspension; b. infecting: feeding young silkworms; 12-24 hours after the young silkworms wake up from the fourth dormancy, spraying and inoculating the spore suspension obtained in the step a; feeding the young silkworms under the conditions that the temperature is 23-25 DEG C and the humidity is more than 95%; after 4 hours, repeatedly spraying and inoculating for one time; c. feeding the young silkworms which are infected in the step b for 48 hours under the conditions that the temperature is 23-25 DEG C and the humidity is more than 95%; feeding the young silkworms in a natural environment until the domestic silkworms grow up, harvesting and drying the silkworms. The stiff silkworm culture method disclosed by the invention can be used for culturing the stiff silkworms in a large scale and has high and stable yield; the quality of the cultured stiff silkworms is excellent and the industrial application prospect is good.

The invention discloses a microbial insecticide composition for control of solenopsis invicta. The microbial insecticide composition consists of beauveria bassiana and thiacloprid, the number of beauveria bassiana spore and the gram of thiacloprid are in a ratio of (8-1)*20 billion:2-9g. Test finds that compounding of beauveria bassiana and thiacloprid has an obvious synergistic effect, can achieve very good control effect, has the advantages of high efficiency and rapidity on solenopsis invicta, is non-toxic to human and livestock, has no pollution to soil and groundwater resources, and is very safe. Therefore, the insecticidal effect of beauveria bassiana is improved, also reinforcement of solenopsis invicta drug resistance is delayed, the dosage of chemical pesticide thiacloprid is reduced, and environmental pollution is reduced, thereby improving the control effect, reducing the dosage of effective ingredients, saving cost and delaying the generation of pest resistance to insecticides.

The invention relates to a natural plant biological composite nutrient solution for killing nematode and a preparation method thereof. The natural plant biological composite nutrient solution for killing nematode comprises bacillus subtilis bacillus subtilis, paecilomyces lilacinus, beauveria bassiana and plants marigold flower, stemona, maidenhair, sophora flavescens, derris trifoliata, melia azedarach and protein liquid according to weight ratio, compared with prior art, the natural plant biological composite nutrient solution has beneficial effects that harm due to soil borne parasites such as root-knot nematode, heterodera sachachtii, and ditylenchus dipsaci can be effectively prevented and treated; no pesticide residue on soil and crops fruits can be realized, usage is convenient, no pollution is generated, nematode harm can be avoided by using the solution for three times in a whole fertility period, and the nutrient solution creates condition for producing green organic food; and has good prevention and treatment effects for crop soil borne disease, can improve the soil and increase soil granular structure, and is capable of preventing soil hardening, increasing the content of the organic matter, dissolving phosphor, releasing potassium and fixating nitrogen, as well as supplementing crop nutrition.

The invention discloses beauveria bassiana with high pathogenicity to anoplophora chinensis larva and applications thereof. The beauveria bassiana strain provided by the invention is named as Beauveria bassiana FJBb201023 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 27, 2014, and the preservation number is CGMCC No.9851. The strain is rapid in growth speed and strong in spore production capability, and has high pathogenicity to anoplophora chinensis larva, and after being inoculated with spore suspension (107 spores/mL), the lethal time of 50% (LT50) of the strain to anoplophora chinensis larva is 5.38 days. A fungal preparation produced by using the strain has a good prophylaxis and treatment effect on anoplophora macularia, and can effectively reduce the woodland population density of anoplophora macularia, therefore, the strain is a biocontrol strain having a potential value in the prevention and control of anoplophora macularia, and has a positive significance to the reduction of damages caused by anoplophora macularia.

Aiming at the defects of conidiospores as propagula of an insecticide of insect pathogenic fungi, the invention provides a technology of liquid culture of entomogenous fungus sclerotia, and the entomogenous fungus can be beauveria bassiana. The liquid culture method of entomogenous fungus sclerotia provided by the invention is required to control the liquid culture components and the culture conditions within a certain range. The cultured sclerotia can be stored for a long time after vacuum drying, and can still germinate mycelia and pathogenic conidiospores under suitable conditions. The micro-sclerotia prepared by drying have maintained activity, can germinate mycelia and spores after rehydration, and increase the potential of beauveria bassiana as a biocontrol factor for western flowerthrips in a soil-dwelling phase. The technology has low cost, long storage period of sclerotia, and is quite suitable for popularization.

The invention provides a safe and circulating disease and pest prevention and control method for organic rice and belongs to the technical field of organic agriculture. The method is characterized in that agricultural prevention and control methods such as rice field-upland field rotation, resistant rice seeds, ploughing and soaking for a long time for killing stem borers and cultivation of strong seedlings are adopted according to rice growth and disease and pest occurrence rules, biological prevention and control methods of stocking of fish fries and tadpoles and biological modelling utilizing a sex attractant are adopted, a physical prevention and control method of mounting of insecticidal lamps and yellow sticky traps is adopted, plants are combined for protecting copper sulphate products, 100 billion spores per g of bacillus subtilis, bordeaux mixture, 40 billion spores per g of beauveria bassiana WG and 0.3% sophocarpidine EC, and special and circulating prevention and control on diseases and pests are carried out. The method provided by the invention has the advantages that the problems that prevention and control means on the diseases and pests of the organic rice are less and the effect is poor are effectively solved, the control efficiency on rice plant hoppers is more than 80%, the control efficiency on rice leaf folders, the stem borers and weevil larvae is more than 90%, the control efficiency on rice leaf blast is 60%, the control efficiency on neck blast is 80%, the large-scale yield increase is 10%, and safe and efficient internal circulation of prevention and control on the diseases and pests is realized, so that the aim of protecting ecology is achieved.

The invention relates to the technical field of identification, separation and purification application of beauveria bassiana strains, and discloses a separation and purification method of beauveria bassiana. By adopting the separation and purification method, a culture medium is scientifically optimized and improved; simple, quick and effective separation and purification of the beauveria bassiana are achieved by combining improvement of cultivation device coverings on the basis of the culture medium with a scientific formula. The method disclosed by the invention is simple and quick to operate, wide in samples applicable to separation and purification of the beauveria bassiana, low in operation cost and free of pollution on environment; the targets of separation and purification can be well achieved.

The invention discloses an artificial preparation method of white muscardine silkworms. The method includes the steps of 1, preparation of beauveria bassiana spore liquid, wherein white muscardine silkworm bodies are soaked and cleaned with sterile water, filtrate is obtained after filtering is completed, and the beauveria bassiana spore liquid is obtained after primary slant culture, secondary slant culture and tertiary slant culture; 2, pathogen toxin counteracting, wherein the beauveria bassiana spore liquid is rapidly sprayed to the silkworm bodies of 5-day-old or older silkworms within 45 min after the beauveria bassiana spore liquid is prepared, and a silkworm room is sealed for 1-1.5 h after the beauveria bassiana spore liquid is sprayed; 3, silkworm body culture, wherein the silkworm room is opened, the silkworm bodies are fed, and the silkworm bodies are fed once every other 3-4 h till the silkworm bodies die; 4, white muscardine silkworm culture, wherein the dead silkworm bodies are transferred to a silkworm rearing bed, the silkworm bodies continue to be cultured under the condition that the temperature is 25-28 DEG C and the humidity is 95-98% till the silkworm bodies are fossilized, and white mycelium conidia grow out; 5, preparation of the finished white muscardine silkworms, wherein white muscardine silkworms with the white mycelium conidia growing out are collected and then dried till the water content is smaller than 10%, and the finished white muscardine silkworms are obtained. The preparation period of the finished white muscardine silkworms is short, and the yield and quality are high.

The invention relates to an agricultural insecticidal composition which takes bacillus thuringiensis and beauveria bassiana as main active components and an application thereof. The agricultural insecticidal composition contains active medical components, a pesticide carrier and an auxiliary agent, wherein the active medical components comprise the bacillus thuringiensis and the beauveria bassiana and the weight part ratio of the bacillus thuringiensis to the beauveria bassiana is 1 to (1-6). The agricultural insecticidal composition provided by the invention has high efficiency and low toxin, and is safe to human, livestock and crops; the crops can be protected and natural enemies are not injured, so that the influences on the ecological environment are small, and a development direction of current pesticides is met; and furthermore, the agricultural insecticidal composition is a green pesticide preparation which has an extremely good development potential.

The invention relates to a fungus metabolism product with destroying intectinal worm's effect and it's preparing method and application in the field of microorganism pesticides technique. The preserved number of the fungus bacterial strain: Beauveria bassiana snf0907 is CGMCC No.1340.