Insecticidal fungus and its application

A technology of fungi and insecticides, applied in application, insecticides, fungi and other directions, can solve the problems of delayed aphid reproduction speed tolerance, inability to control insect pests in time, slow onset of host effect, etc., to ensure the safety of agricultural products, The effect of protecting crops and reducing the amount of application

Inactive Publication Date: 2012-02-29
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that Beauveria bassiana, like other entomopathogenic fungi, has the weakness of being slow to act on the host. It usually takes 3-5 days to reach the peak of death in biological assays, which is relatively lagging behind the reproduction speed of aphids and the plant's response to aphids. The degree of tolerance to harm, so often can not control the pests in time, can not achieve the ideal control effect that people expect

Method used

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  • Insecticidal fungus and its application
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  • Insecticidal fungus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and purification of Beauveria bassiana BBNS-J9-16 (Beauveria bassiana) bacterial strain

[0037] Soil and corpses infected with Beauveria bassiana were collected from natural forests, grasslands or farmland in Inner Mongolia, Anhui, Jiangxi, Guizhou and other places, and the microorganisms were isolated and purified in the laboratory.

[0038] The separation and purification steps are: 1) Isolate the target microorganism from the soil: dilute the soil 20 times, 100 times, and 500 times with sterile water, inoculate them on selective medium respectively, cultivate them at 25°C for 4-7 days, pick out Transfer the colony to a new selective medium plate, culture a single colony for 7-10 days, and identify Beauveria bassiana by colony appearance and microscopic morphology inspection, transfer to the culture medium test tube, at 25 ℃ After culturing at high temperature for 14 days, they were stored in a refrigerator at 4°C. 2) Isolate target microorganism...

Embodiment 2

[0040] Biomorphological characteristics of the strain of embodiment 2

[0041] Prepare PDAY medium, its components and preparation method are to boil 200g of fresh potatoes and about 1000mL of water for 15min, filter with gauze and retain the liquid, add 20g of glucose, 5g of yeast extract powder, and 15g of agar, heat to completely melt the agar, and quantify to 1000mL, divided into Erlenmeyer flasks, placed in a high-pressure steam sterilizer at 121°C for 20min, and when cooled to about 60°C, pour it into a petri dish to make a plate for use.

[0042] Inoculate conidia on PDAY plate medium, culture at 25°C for 2 days, colonies can be seen with the naked eye, and the mycelia of the colonies are white and fluffy. Continue to cultivate for 3-4 days, and it can be seen that the colonies expand and grow, and milky white spores are produced on the surface. After the spores mature, White powdery spores were enriched on the surface of the colony.

[0043] Drop a drop of sterilized ...

Embodiment 3

[0044] Ribosome ITS region nucleotide sequence characteristics of embodiment 3 bacterial strains

[0045] Prepare LM medium, its components and preparation method are as follows: weigh 40g of glucose and 5g of peptone, add water to 1000mL, divide into Erlenmeyer flasks, place in a high-pressure steam sterilizer at 121°C for 20min, and let cool to room temperature for later use.

[0046] Inoculate the conidia into the above-mentioned medium, the inoculum size is 1×10 5 Spores / mL, cultured on a shaker at 25±1°C and 200rpm for 48h, filtered through filter paper, retained mycelia, squeezed dry for later use.

[0047] Genomic DNA was extracted by CTAB method. According to the routine DNA extraction operation, the genomic DNA is obtained, such as figure 2 shown.

[0048] ITS1-5.8S-ITS2 rDNA region primer 15'-TCCGTAGGTGAACCTGCGG and primer 25'-TCCTCCGCTTATTGATATGC were used for PCR amplification to obtain amplified DNA fragments, such as image 3 As shown, the fragment was subje...

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Abstract

The invention relates to the field of biological control, especially to an insecticidal fungus and its application. The invention provides an insecticidal fungus Beauveria bassiana (Balsamo) Vuillemin strain BBNS-J9-16(Beauveria bassiana), the collection number of which is CGMCC No. 5288. The invention also provides an application of the Beauveria bassiana (Balsamo) Vuillemin strain for killing aphid, and provides a efficient composite pesticide, which contains the above Beauveria bassiana (Balsamo) Vuillemin strain BBNS-J9-16 thalline and / or its spore powder. The high-efficient composite pesticide provided by the invention is used to infect hosts by the utilization of the insect fungus which is environment friendly and compatible with the environment so as to control insects. The insecticidal fungus can be used to protect crops and minimize the use of chemical pesticides, and plays an active role in guaranteeing the agricultural product safety and agro-environmental safety.

Description

technical field [0001] The invention relates to the field of biological control, in particular to an insecticidal fungus and its application. Background technique [0002] Aphids have the characteristics of small size, fast growth, high reproduction rate, strong drug resistance, and strong adaptability to the environment. They are widely distributed in various agricultural and forestry plants around the world. It not only eats harmful plants directly, but also spreads plant viruses indirectly, causing serious economic losses to the production of vegetables, grain and oil, fruit trees, and gardens. The aphid species on vegetables mainly include peach red aphid Myzus persicae (Sulzer), radish aphid Lipaphis erysimi (Kaltenbach), cabbage aphid (Pipella aphid) Brevicoryne brassicae (Linn.). Green peach aphid is distributed nationwide and eats a wide variety of foods. It can harm more than 400 species of plants in 50 families, mainly including cruciferous, solanaceous vegetables...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 农向群张泽华张英财王广君曹广春
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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