Agricultural insecticidal composition and application thereof

A technology of insecticidal composition and strain, applied in the field of pesticides, can solve the problems of high requirements on the application environment and failure, and achieve the effects of human, animal and crop safety, crop protection, and high insecticidal rate and control efficiency.

Inactive Publication Date: 2016-07-06
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The single use of Beauveria bassiana for insecticide has the disadvantage of high requireme

Method used

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  • Agricultural insecticidal composition and application thereof
  • Agricultural insecticidal composition and application thereof
  • Agricultural insecticidal composition and application thereof

Examples

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preparation example Construction

[0038] The preparation method of Bacillus thuringiensis described in the present invention preferably comprises the following steps:

[0039] a) liquid activation of Bacillus thuringiensis strains;

[0040] b) inoculating the activated Bacillus thuringiensis in the Bacillus thuringiensis liquid culture medium to carry out liquid fermentation, and stop the fermentation when the total number of live bacteria reaches 200-900 million / ml, and obtain the Bacillus thuringiensis fermentation liquid;

[0041] c) inoculating the Bacillus thuringiensis fermentation liquid obtained in the step b) into the Bacillus thuringiensis solid medium 2, and performing solid fermentation of the Bacillus thuringiensis to obtain solid fermentation Bacillus thuringiensis.

[0042] The activation of the bacillus thuringiensis strain in the present invention is to inoculate the preserved bacillus thuringiensis strain in an eggplant-shaped bottle equipped with a nutrient agar medium, and cultivate it at 28-...

Embodiment 1

[0055] Preparation of solid powdery Beauveria bassiana: inoculate the preserved Beauveria bassiana strains in an eggplant-shaped bottle equipped with nutrient agar medium, and cultivate for 10 hours at 28° C. to activate the culture; the Beauveria bassiana after the activation culture is inoculated at 121° C. In the liquid medium after sterilization for 35 minutes, carry out liquid fermentation in a fermenter, specific fermentation conditions: temperature 27°C; pH value 6.9; tank pressure 0.08MPa, fermentation ventilation 1V / V min; The fermentation time is preferably 32 hours; after 30 hours of fermentation, the total number of fermented viable bacteria is detected, and when the total number of viable bacteria reaches 9.5 billion / ml, the fermentation is stopped to obtain a Beauveria bassiana fermentation liquid; the obtained Beauveria bassiana fermentation liquid is inoculated on a solid medium Among them, the specifically described solid medium includes the following component...

Embodiment 2

[0057] Preparation of solid powdery Bacillus thuringiensis: Inoculate the preserved Bacillus thuringiensis strains in an eggplant-shaped bottle equipped with nutrient agar medium, cultivate at 30°C for 10h for activation culture; inoculate the Bacillus thuringiensis after activation culture at 121°C and sterilize for 35min In the final liquid culture medium, carry out liquid fermentation, specific fermentation condition: temperature is 30 ℃; Fermentation time is 32 hours; After 24 hours of fermentation, detect the total number of fermented live bacteria, stop fermentation when the total number of live bacteria reaches 200 million / ml, Obtain Bacillus thuringiensis fermented liquid; Inoculate the obtained Bacillus thuringiensis fermented liquid in solid medium, specifically described solid medium comprises the following components by weight: bran 70%, soybean meal 25%, corn flour 3%, KH 2 PO 4 0.2%, MgSO 4 0.05%, CaCO 3 1.75%, the inoculation amount of Bacillus thuringiensis i...

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Abstract

The invention relates to an agricultural insecticidal composition which takes bacillus thuringiensis and beauveria bassiana as main active components and an application thereof. The agricultural insecticidal composition contains active medical components, a pesticide carrier and an auxiliary agent, wherein the active medical components comprise the bacillus thuringiensis and the beauveria bassiana and the weight part ratio of the bacillus thuringiensis to the beauveria bassiana is 1 to (1-6). The agricultural insecticidal composition provided by the invention has high efficiency and low toxin, and is safe to human, livestock and crops; the crops can be protected and natural enemies are not injured, so that the influences on the ecological environment are small, and a development direction of current pesticides is met; and furthermore, the agricultural insecticidal composition is a green pesticide preparation which has an extremely good development potential.

Description

technical field [0001] The invention relates to the field of pesticides, in particular to an agricultural insecticidal composition and its application. Background technique [0002] Agriculture is the foundation of the national economy, the source of human food and clothing, and the foundation of survival. The development of agriculture directly affects the overall development of the national economy. In agricultural production, pests will seriously affect the growth of crops, affect agricultural harvest, and bring serious economic losses to farmers. Therefore, effective control of crop pests is of great significance to ensure the economic interests of farmers and food security. [0003] At present, there are many kinds of pests that harm crops, mainly insects, Lepidoptera pests and Coleoptera pests, such as diamondback moth, cotton bollworm, corn borer, scarab, and underground pests such as grubs and mole crickets. Agricultural pesticides in the prior art can be classified...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N63/04A01P7/00
CPCA01N63/00A01N63/30A01N2300/00
Inventor 李肖宇梁小文王根豪严艺波吴俊杰石峥曾升华李英武
Owner JIANGXI TIANREN ECOLOGY
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