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Sclerotium rolfsii solid fermentation culture medium and application thereof

A technology of neat Sclerotinia and solid fermentation, applied in the direction of application, fungi, and microorganism-based methods, can solve the problem of high bran price, and achieve the effects of simple preparation process, cost reduction, and pollution reduction

Active Publication Date: 2013-06-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previously, although bran was used as a substrate to obtain a higher fermentation effect, the relative price of bran was relatively high. Therefore, it is necessary to find an alternative medium with lower cost and better fermentation effect

Method used

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  • Sclerotium rolfsii solid fermentation culture medium and application thereof
  • Sclerotium rolfsii solid fermentation culture medium and application thereof
  • Sclerotium rolfsii solid fermentation culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0032] Embodiment 2 (composite embodiment of bran and agricultural waste)

[0033] Preparation of solid fermentation medium:

[0034] Different agricultural wastes and bran were mixed as a solid matrix at a weight ratio of 70:30, and after thorough mixing, the preparation was carried out according to the method in Comparative Example 1.

[0035] Biological herbicide preparation:

[0036] The prepared mixed agricultural waste-bran medium was inoculated and cultured according to the method in Comparative Example 1 to obtain various agricultural waste-bran medium herbicides containing mycelium.

[0037] Fermentation effect implementation 2 (example of compounding bran and agricultural waste)

[0038] According to the method in Example 1 of Comparative Fermentation Effect, each herbicide containing mycelium bran-agricultural waste medium prepared in Example 2 was tested. The test results are shown in Table 2 below.

[0039] Table 2 Effects of different agricultural waste-bran ...

Embodiment 3

[0042] Embodiment 3 (bran-rice husk composite embodiment)

[0043] Preparation of solid fermentation medium:

[0044] Different proportions of bran and rice husk were used as the solid matrix, and after thorough mixing, the preparation was carried out according to the method in Comparative Example 1.

[0045] Biological herbicide preparation:

[0046] The bran-rice husk mixed media prepared were inoculated and cultured according to the method in Comparative Example 1 to obtain the bran-rice husk herbicides containing mycelium.

[0047] Fermentation effect implementation 3 (bran-rice husk compound example)

[0048] According to the method in Example 1 of comparative fermentation effect, each bran-rice husk medium containing mycelium prepared in Example 3 was tested. The test results are shown in Table 3 below.

[0049] Table 3 The influence of oxalic acid production and pathogenicity on the fermented oxalic acid production and pathogenicity of whole bran and rice husk mixed...

Embodiment 4

[0052] Embodiment 4 (bran-rice husk composite water content embodiment)

[0053] Preparation of solid fermentation medium:

[0054] The bran is added to the rice husk according to 50% of the weight of the solid matrix, and the humidity of the matrix is ​​adjusted. There are five treatments of 20%, 25%, 30%, 35%, and 40%. Solid fermentation media with different water contents are prepared.

[0055] Biological herbicide preparation:

[0056] The prepared medium with different water contents was inoculated and cultured according to the method in Comparative Example 1 to obtain the herbicide containing mycelium.

[0057] Fermentation effect example 4 (bran-rice husk composite water content example)

[0058] According to the method in Example 1 of Comparative Fermentation Effect, the media with different water contents prepared in Example 4 were tested. The test results are shown in Table 4 below.

[0059] Table 4 Effect of initial moisture content of bran-rice husk medium on o...

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Abstract

The invention discloses a sclerotium rolfsii solid fermentation culture medium and an application thereof. The solid fermentation culture medium comprises a solid substrate and water, wherein the solid substrate comprises wheat bran and agricultural wastes, the wheat bran accounts for 20%-60% of the total weight of the solid substrate, and the weight ratio of the water to the solid substrate is (20-45):100; and the agricultural wastes select rice husk, sweet sorghum straw, an organic fertilizer, a wheat bran substrate, vinegar residue, wine lees, garden dead twigs or sawdust. The sclerotium rolfsii solid fermentation culture medium prepared by the preparation method disclosed by the invention is low in cost, the preparation process is simple, and a herbicide prepared by adopting the solid fermentation culture medium can be preserved at room temperature.

Description

technical field [0001] The invention relates to a solid fermentation medium for Sclerotinia sclerotiorum, in particular to a solid fermentation medium for Sclerotinia sclerotium using agricultural waste and bran as raw materials and an application thereof. Background technique [0002] The continuous application of chemical herbicides has exposed many disadvantages. The development and utilization of microorganisms, especially plant pathogenic fungi, for weed control is an effective, safe, environmentally friendly and sustainable weed management method. To investigate, isolate and use weed pathogenic bacteria for biological control is a hot spot in weed research in the world. Since S. sclerotiorum of the genus Sclerotinia (Sclerotinia spp.) can cause rapid plant disease and kill a variety of broad-leaved weeds at the same time, it has been screened for development as a biological herbicide for lawn control Dandelion (Taraxacum officinale), thistle (Cirsium arvense) and othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P13/02C12R1/645
Inventor 强胜唐伟庄超
Owner NANJING AGRICULTURAL UNIVERSITY
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