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Application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control

A technology of Alternaria and Solanum blight, applied in the field of microorganisms, can solve the problems of unsafe microbial source pesticide types and other problems

Inactive Publication Date: 2011-10-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of endophytic fungus-Alternaria alternata (Alternaria alternata) that isolates and screens out from camphor tree for chemical pesticide unsafe and microbial source pesticide kind less; Another purpose of the present invention is Provides a method for preventing and treating plant fungal diseases by utilizing the metabolites of the strain

Method used

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  • Application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control
  • Application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control
  • Application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control

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Embodiment 1

[0018] Embodiment 1: (isolation and screening of Alternaria 31)

[0019] The present invention collected camphor tree stems in Tianmu Mountain, Hangzhou, Zhejiang in June 2008, and obtained Alternaria sp. 31. Save.

[0020] Determination of the antibacterial activity of Alternaria 31: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. Put 1 piece of bacterium cake for testing such as cucumber blight fungus (Rhizoctonia solani) or watermelon wilt fungus (Fusarium oxysporium) or tomato gray mold fungus (Botrytis cinerea) with a diameter of 4mm respectively, and the fungus cake is connected to the center of the petri dish (petri dish 9 cm in diameter), placed in an incubator for 72 hours at 28°C, treated with sterile water as a control, and repeated 3 times; the diameter of the colony was measured by the cross method, and the inhibition rate was calculated by the foll...

Embodiment 2

[0023] Embodiment 2: (the preparation method of Alternaria 31 fermentation metabolite)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the isolated and preserved Alternaria 31 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C ± 1°C until the hyphae cover the entire petri dish. A 4mm hole puncher is punched to obtain a fungus cake for inoculation;

[0026] (2) Fermentation culture: The fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH per 1000mL. 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 126 hours;

[0027] (3) Extraction of fermented product: After the fermentation is ...

Embodiment 3

[0029] Embodiment 3: (the inhibitory effect of Alternaria 31 metabolites to cucumber solanum blight, watermelon wilt and tomato Botrytis cinerea)

[0030] (1) Accurately weigh 0.18 g of the metabolite of Alternaria 31, add 10 mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother solution with a concentration of 18 g / L;

[0031] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1800mg / L and 900mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C (Final concentrations of Alternaria 31 metabolites are 180mg / L and 90mg / L), and after cooling, place a 4mm-diameter watermelon wilt or cucumber blight or tomato Botrytis cinerea on each medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), placed in an incubator...

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Abstract

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of the biocontrol endophytic fungus Alternaria alternata and the application of its metabolites. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant ...

Claims

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Application Information

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IPC IPC(8): A01P3/00A01N63/04
Inventor 俞晓平申屠旭萍董胜张郝培应边亚琳马正
Owner CHINA JILIANG UNIV
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