40 results about "Alternaria sp" patented technology

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.

The invention relates to an anthraquinone dimer derivative and a preparation method and application thereof. The preparation method comprises the following steps of: performing strain culture on endophytic fungi Alternaria sp. (ZJ-2008003) from alcyonacea Sarcophyton sp. in South China Sea in a strain culture medium, performing fermentation cultivation in a fermentation medium, filtering the obtained fermentation solution to obtain thalli, and extracting the obtained thalli by using methanol; and concentrating the extract, performing silica-gel column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography preparation, concentrating the obtained eluate, and thus obtaining red powder, namely the anthraquinone dimer derivative Alterporriol P. The anthraquinone dimer derivative from the marine fungi has a strong effect of resisting cell proliferation of human prostatic cancer PC-3 and human colon cancer HCT-116, and can be used as a cell proliferation inhibitor or an anti-tumor agent; and the raw material can be produced in large scale, so the derivative has a broad application prospect.

The invention relates to anthraquinone dimer derivatives and a preparation method and application thereof. The preparation method comprises the steps of firstly conducting strain culture of an endophytic fungus Alternaria sp. (ZJ-2008003) coming from South China Sea soft coral Sarcophyton Sp. in a strain culture medium, conducting fermentation culture in a fermentation culture medium, filtering the obtained fermentation liquid, removing filtrate to obtain thalli, extracting the thalli by using methanol, concentrating the extract, conducting silica gel column chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatographic preparation, concentrating the eluent to obtain red powder, which is the anthraquinone dimer derivatives alterporriol Q and alterporriol R. The anthraquinone dimmer derivatives coming from marine fungi have a strong antiviral effect, can effectively inhibit porcine reproductive and respiratory syndrome viruses (PRRSV) and can be used as antiviral drugs, raw materials can be produced in a large scale and the application prospect is wide.

The invention relates to an anthraquinone derivative, a preparation method and application thereof. During preparation, entomopathogenic fungi in soft coral Alternaria sp. (ZJ-2008003) is first placed in a strain culturing medium for strain culturing and then placed in a fermentation culturing medium for fermentation culturing, obtained fermentation liquor is filtered, thalli are removed to obtain filtrate, and the filtrate is extracted through ethyl acetate; after extract liquor is concentrated, silicagel column chromatography, sephadex LH-20 gel column chromatography and half-preparation of high performance liquid chromatography preparation are conducted, obtained eluent is concentrated, and clear crystals are obtained, namely, the compound of tetrahydroaltersolanols C-E; pink powder is obtained, namely, the compound of tetrahydroaltersolanol F. The anthraquinone derivative originating from marine fungi has strong antiviral effect, can effectively restrain porcine reproductive and respiratory syndrome virus (PRRSV), can be used as antiviral drug, and has broad application prospects due to the fact that raw materials can be produced in a large scale.

The invention discloses an Alternaria sp strain GW61 CGMCC No. 21070 and application thereof in preparing Alternaria phenol. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC for short, address: #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing) as a patent strain on November 27, 2020, and the registration number of the strain in the preservation center is CGMCC No. 21070. The strain effectively solves the technical problem in the industry of preparing high-purity Alternaria phenol standard products.

The invention relates to endophytic fungus capable of promoting phosphorus absorption of aleurites montana. The endophytic fungus is alternaria sp. ZY4 and has been registered and preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 30th, 2015. The preservation number is CGMCC No. 10328. According to the invention, the endophytic fungus ZY4 of aleurites montana is inoculated to land culture seedling of aleurites montana by a bacteria liquid spraying method, and content of phosphor in aleurites montana is measured after 60 days of inoculation. The difference of phosphorus content between aleurites montana treated by the inoculated strain ZY4 and a contrast is significant. The strain ZY4 treatment is more beneficial to promote phosphorus absorption of a plant than a contrast.

The invention relates to a marine fungi fermentation extract and application thereof as an anti-senile dementia drug. The extract is prepared according to the following steps: (1) under aseptic conditions, inoculating marine fungi Alternaria sp. W-1 into a PD liquid culture medium, and carrying out constant temperature shaking table shaking culture to obtain a seed solution, wherein the temperature is 25 to 30 DEG C, the shaking table rotating speed is 150 to 180 rpm, and the culture time is 2 to 4 d; (2) inoculating the obtained seed solution obtained in the step (1) into a czapek's medium toobtain a fermentation matter, wherein the pH of the medium is 4.0 to 8.0, the inoculum amount is 5% to 20%, the culture temperature is 22 to 34 DEG C, and the culture time is 6 to 14 d; and (3) filtering the fermentation matter obtained in the step (2) with three to five layers of sterile gauze to obtain a filter liquor, extracting for two to four times with ethyl acetate after concentration through a rotary evaporator, merging an organic phase, and thus obtaining the extract after concentration through the rotary evaporator.

The invention belongs to the technical field of microbial engineering and pharmaceutical engineering and particularly relates to alternarlactam extracted from a solid fermentation material of maubertiana boott endophytic fungi (Alternaria sp.) and a preparation method and anti-tumor effect thereof. The alternarlactam can be prepared by a solid fermentation method and a chemical synthesis method. The alternarlactam has the effects of strongly inhibiting the proliferation of human cervical cancer cells HeLa and human liver cancer cells QSG-7701, so that the alternarlactam can be used as a compound having the anti-tumor effect and is applied to preparing anti-tumor medicaments.