40 results about "Alternaria sp" patented technology

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

The invention relates to an anthraquinone derivative, a preparation method and application thereof. During preparation, entomopathogenic fungi in soft coral Alternaria sp. (ZJ-2008003) is first placed in a strain culturing medium for strain culturing and then placed in a fermentation culturing medium for fermentation culturing, obtained fermentation liquor is filtered, thalli are removed to obtain filtrate, and the filtrate is extracted through ethyl acetate; after extract liquor is concentrated, silicagel column chromatography, sephadex LH-20 gel column chromatography and half-preparation of high performance liquid chromatography preparation are conducted, obtained eluent is concentrated, and clear crystals are obtained, namely, the compound of tetrahydroaltersolanols C-E; pink powder is obtained, namely, the compound of tetrahydroaltersolanol F. The anthraquinone derivative originating from marine fungi has strong antiviral effect, can effectively restrain porcine reproductive and respiratory syndrome virus (PRRSV), can be used as antiviral drug, and has broad application prospects due to the fact that raw materials can be produced in a large scale.

The invention relates to anthraquinone dimer derivatives and a preparation method and application thereof. The preparation method comprises the steps of firstly conducting strain culture of an endophytic fungus Alternaria sp. (ZJ-2008003) coming from South China Sea soft coral Sarcophyton Sp. in a strain culture medium, conducting fermentation culture in a fermentation culture medium, filtering the obtained fermentation liquid, removing filtrate to obtain thalli, extracting the thalli by using methanol, concentrating the extract, conducting silica gel column chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatographic preparation, concentrating the eluent to obtain red powder, which is the anthraquinone dimer derivatives alterporriol Q and alterporriol R. The anthraquinone dimmer derivatives coming from marine fungi have a strong antiviral effect, can effectively inhibit porcine reproductive and respiratory syndrome viruses (PRRSV) and can be used as antiviral drugs, raw materials can be produced in a large scale and the application prospect is wide.

The invention belongs to the technical field of microbial engineering and pharmaceutical engineering and particularly relates to alternarlactam extracted from a solid fermentation material of maubertiana boott endophytic fungi (Alternaria sp.) and a preparation method and anti-tumor effect thereof. The alternarlactam can be prepared by a solid fermentation method and a chemical synthesis method. The alternarlactam has the effects of strongly inhibiting the proliferation of human cervical cancer cells HeLa and human liver cancer cells QSG-7701, so that the alternarlactam can be used as a compound having the anti-tumor effect and is applied to preparing anti-tumor medicaments.

A alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6380, and the name of the strain is 11775-42-Z1, and is 42# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 42# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 42# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.

The present invention relates to a kelp endophytic fungus and its application in the preparation of biologically active extracts. The strain preservation information of the kelp endophytic fungus is: name of depository unit: General Microbiology Center of China Microbial Strain Preservation Management Committee; preservation Unit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; date of deposit: January 2, 2018; deposit number: CGMCC No.15181; classification name: Alternaria sp.

The invention discloses a monoclonal antibody that recognizes Alternaria and a hybridoma cell line thereof. The monoclonal antibody that recognizes Alternaria is secreted by a hybridoma cell strain with a deposit number of CCTCC No: C2019218 ; The hybridoma cell line is named AaA1, and was deposited in the China Center for Type Culture Collection (CCTCC) on October 17, 2019, and the preservation number is: CCTCC No: C2019218. This monoclonal antibody is used for the identification and dynamic monitoring of animal and plant diseases caused by Alternaria infection and the biological research of Alternaria. It can be obtained by intraperitoneal injection of this cell line into BaL b / c mice Compared with polyclonal antibodies, a large number of monoclonal antibodies have the advantages of high purity, strong specificity, and good repeatability.

The invention relates to a preparation method and application of two sesquiterpene hybrid hydroquinone compounds. The sesquiterpene hybrid hydroquinone compounds with novel structures are produced by adopting alternaria sp. SP-32 in a South China Sea sponge sample. Proved by experiments, the compounds have flu virus A H1N1 resistant effect, and have potential for developing flu virus A resistant medicament preparations.

The present invention relates to the endophytic fungus strain HUBU0122 of Dirtwort and its application. The endophytic fungus named Alternaria ( Alternaria sp. ) HUBU0122, whose strain preservation unit is the China Type Culture Collection Center, the preservation address is No. 299 Bayi Road, Wuhan City, Hubei Province, the preservation date is April 19, 2021, and the preservation number is CCTCC No.M2021414. The bacterium has antibacterial effect on Salmonella and Xanthomonas oryzae, and can be used as a utilization resource of plant endophytic fungi, laying the foundation for obtaining new agricultural antibiotic natural ingredients that resist plant pathogenic fungal diseases.

The invention relates to an anthraquinone dimer derivative and a preparation method and application thereof. The preparation method comprises the following steps of: performing strain culture on endophytic fungi Alternaria sp. (ZJ-2008003) from alcyonacea Sarcophyton sp. in South China Sea in a strain culture medium, performing fermentation cultivation in a fermentation medium, filtering the obtained fermentation solution to obtain thalli, and extracting the obtained thalli by using methanol; and concentrating the extract, performing silica-gel column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography preparation, concentrating the obtained eluate, and thus obtaining red powder, namely the anthraquinone dimer derivative Alterporriol P. The anthraquinone dimer derivative from the marine fungi has a strong effect of resisting cell proliferation of human prostatic cancer PC-3 and human colon cancer HCT-116, and can be used as a cell proliferation inhibitor or an anti-tumor agent; and the raw material can be produced in large scale, so the derivative has a broad application prospect.

The present invention relates to a marine fungus fermented extract and its application as an anti-senile dementia drug. The extract is prepared by the following steps: (1) under sterile conditions, the marine fungus Alternaria sp.W‑1 is inoculated into PD In the liquid medium, vibrate culture on a constant temperature shaker, the temperature is 25-30°C, the speed of the shaker is 150-180r / min, and the culture time is 2-4d to obtain a seed solution; (2) the seeds obtained in step (1) The solution is inoculated into the Cha's medium, the pH of the medium is 4.0-8.0, the inoculum size is 5-20%, the culture temperature is 22-34°C, and the culture time is 6-14d to obtain a fermented product; (3) the step ( 2) The fermented product obtained is filtered with 3-5 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator, extracted 2-4 times with ethyl acetate, combined with the organic phases, concentrated by a rotary evaporator to obtain the extracted thing.

The invention relates to an M-4 strain for degradation of waste castor-based lubricating oil and mineral oil. The M-4 strain is characterized in that a category name of the M-4 strain is Alternaria sp.; a preservation number is CGMCC No. 4933; a preservation date is June 10, 2011; the preservation organization name is China general microbiological culture collection center; and the preservation address is No. 3, Court 1, Beichen West Road, Chaoyang District, Beijing City. Through a plate gradient coating separation method, a soil sample and a water sample are collected from a place around a sewage outlet of a castor oil squeezing workshop and a microorganism which grows through depending on castor-based lubricating oil as an only carbon source is screened out. The plate gradient coating separation method has simple, convenient and fast operation processes. The M-4 strain for degradation of castor-based lubricating oil can grow fast, is suitable for expansion re-culture under the conditions of an optimum culture temperature and a pH value and can be prepared into a strain starter to be dropped into a polluted environment for ecological restoration.