Marine fungi fermentation extract and application thereof as anti-senile dementia drug
A technology of marine fungi and extracts, which is applied in the application field of marine fungal fermented extracts and anti-senile dementia drugs, achieving significant inhibitory activity, strong antioxidant activity, and improved biological activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] (1) Under sterile conditions, the marine fungus Alternaria sp.W-1 was inoculated into PD liquid medium, and cultured on a constant temperature shaker at a temperature of 28°C, the speed of the shaker was 160r / min, and the culture time was 3 days. get seed liquid;
[0035] (2) Inoculate the seed solution obtained in step (1) into the Cha's culture medium (the fermentation volume is 2.0 L), the inoculum volume is 10%, the cultivation temperature is 28° C., and the cultivation time is 10 days to obtain a fermented product;
[0036] (3) Filter the fermented product obtained in step (2) with 4 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator, extracted 3 times with ethyl acetate, combined with the organic phases, concentrated by a rotary evaporator to obtain the obtained Said extract (2.8g, referred to as product A).
Embodiment 2
[0037] The acetylcholinesterase inhibitory activity (AchE) of embodiment 2 product A and the mensuration of antioxidant
[0038] Take an appropriate amount of product A and dissolve it in DMSO to prepare a solution with a mass concentration of 5 mg / mL. The method for measuring the AChE activity inhibition rate refers to the improved Ellman method, and the method for measuring the DPPH free radical scavenging ability and superoxide anion scavenging ability refers to the literature. The results are shown in Table 1
[0039] Table 1
[0040]
Embodiment 3
[0041] Example 3 Marine fungus Alternaria sp.W-1 fermentation condition optimization
[0042] Follow the same steps to prepare the seed solution: under sterile conditions, inoculate the marine fungus Alternaria sp.W-1 into PD liquid medium, and culture it on a constant temperature shaker with shaking at 25-30°C and the speed of the shaker at 150- 180r / min, the cultivation time is 2-4d, to obtain the seed liquid; follow the same steps to obtain the extract from the fermented product: the fermented product is filtered with 3-5 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator and then washed with acetic acid Ethyl ester was extracted for 2-4 times, the organic phases were combined, and concentrated by a rotary evaporator to obtain the extract. Investigate the effects of temperature, pH, inoculum size, and fermentation time on the acetylcholinesterase inhibitory activity of the fermentation extract in the fermentation culture (that is, i...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com