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Alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content

A technology of Alternaria and Salvia, applied in the field of biology

Inactive Publication Date: 2014-08-20
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technical method is simple to operate, green and environmentally friendly, and has a wide range of application prospects. At present, there are no research reports on the use of fungi and salvia miltiorrhiza in symbiotic cultivation to increase the yield of salvia miltiorrhiza and the content of phenolic acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Solid culture of 42#: wheat bran: 100g, sawdust: 150g, KH 2 PO 4 : 2g, MgSO 4 : 1g, CaSO 4 : 0.7g, glucose: 14g, corn cob: 20g, corn crumbs: 30g, add water and mix evenly, divide into bottles, sterilize at 122°C for 140min, let cool for later use. Take the 42# bacterial slices cultured on PDA medium, place them in the middle of the solid medium, and cultivate them in a dark room at 25°C until the 42# bacteria grow to 2 / 3 of the medium in the bottle before use.

[0013] Bacteria seedling symbiosis cultivation: use the method of greenhouse potting, when the salvia miltiorrhiza is cultivated in spring, take out the salvia miltiorrhiza seedlings 20-30 days after budding, and apply 0.5g of 42# bacterial material cultured in solid to the root of each salvia miltiorrhiza seedling, 1 pot per pot Plants are potted in the greenhouse after being covered with soil, planted and managed by conventional methods, and harvested after 6 months.

[0014] Weigh dry weight, measure the ...

Embodiment 2

[0016] Solid culture of 42#: wheat bran: 170g, sawdust: 170g, KH 2 PO 4 : 3g, MgSO 4 : 2g, CaSO 4 : 1g, glucose: 22g, corncob: 40g, corn crumbs: 50g, add water and mix evenly, divide into bottles, sterilize at 122°C for 140min, let cool for later use. Take the 42# bacterial slices cultured on PDA medium, place them in the middle of the solid medium, and cultivate them in a dark room at 25°C until the 42# bacteria grow to 2 / 3 of the medium in the bottle before use.

[0017] Bacteria seedling symbiosis cultivation: use the method of field cultivation, when cultivating Salvia miltiorrhiza in spring, take out the seedlings of Salvia miltiorrhiza 20-30 days after budding, and apply 1.0g of 42# bacterial material cultured in solid to the root of each salvia miltiorrhiza seedling, and cover it with soil Plant in the field, carry out field planting management by conventional methods, and harvest after 4 months.

[0018] Measure the content of total phenolic acid and salvianolic ac...

Embodiment 3

[0020] Solid culture of 42#: wheat bran: 135g, sawdust: 160g, KH 2 PO 4 : 2.5g, MgSO 4 : 1.5g, CaSO 4 : 0.85g, glucose: 18g, corn cob: 30g, corn crumbs: 40g, add water and mix evenly, divide into bottles, sterilize at 122°C for 140min, let cool for later use. Take the 42# bacterial slices cultured on PDA medium, place them in the middle of the solid medium, and cultivate them in a dark room at 25°C until the 42# bacteria grow to 2 / 3 of the medium in the bottle before use.

[0021] Bacteria seedling symbiosis cultivation: use the method of field cultivation, when cultivating Salvia miltiorrhiza in spring, take out the seedlings of Salvia miltiorrhiza 20-30 days after budding, and apply 1.5g of 42# bacterial material cultured in solid to the root of each salvia miltiorrhiza seedling, and cover it with soil Plant in the field, carry out field planting management by conventional methods, and harvest after 6 months.

[0022] Weigh dry weight, measure the content of total phenol...

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Abstract

A alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6380, and the name of the strain is 11775-42-Z1, and is 42# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 42# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 42# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a fungus for increasing the yield and active ingredient content of salvia miltiorrhiza. Background technique [0002] Medicinal salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge., which was first recorded in "Shen Nong's Materia Medica", and ranks among the top grades. It has the effects of promoting blood circulation, cooling blood, clearing heart and eliminating troubles, and is widely used clinically in the treatment of coronary heart disease. Danshen preparation is also the basic medicine for the treatment of cardiovascular and cerebrovascular diseases in my country. Due to its definite curative effect, Salvia miltiorrhiza has become a traditional Chinese medicine with large consumption and high sales in my country. [0003] Salvianolic acids are water-soluble components of Salvia miltiorrhiza, which have protective effects on heart, brain, liver, kidney,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 郭顺星唐坤王春兰李标陈晓梅
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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