46 results about "Cladosporium sp" patented technology

The invention provides application of endophytic cladosporium fungus of liriope spicata var.prolifera Y.T.MA in preparing steroid saponin, and relates to the technical field of microorganisms. The application is mainly used for solving the problems that most existing steroid saponin of liriope spicata var.prolifera Y.T.MA is from extracts of liriope spicata var.prolifera Y.T.MA, tubers of liriopespicata var.prolifera Y.T.MA are long in production period, low in steroid saponin content and high in saponin extraction cost, and accordingly the market volume of the steroid saponin of liriope spicata var.prolifera Y.T.MA is limited. The fungus is separated from living tubers of liriope spicata var.prolifera Y.T.MA, authenticated as Cladosporium sp.EF111 by means of the 18 S rDNA sequence, andpreserved in the China Center for Type Culture Collection. The preservation date is December 7th, 2016, and the preservation number of CCTCC No:M2016725. According to the application, liriope spicatavar.prolifera Y.T.MA produced by a liriope spicata var.prolifera Y.T.MA GAP base which is the original production place of liriope spicata var.prolifera Y.T.MA in Oumiao town, Xiangyang city in Hubeiprovince, endophytic fungus strain liquid of liriope spicata var.prolifera Y.T.MA is used for fermentation, and the steroid saponin of liriope spicata var.prolifera Y.T.MA is produced. The endophyticfungus is an important microorganism for finding new resources of steroid saponin of liriope spicata var.prolifera Y.T.MA.

The invention discloses a Cladosporium sp. strain, and an extract and application thereof. The Cladosporium sp. FS86 is collected to China Center for Type Culture Collection (CCTCC) on March 27th, 2014, wherein the address is Wuhan University, Wuhan City, PRC, and the collection number is CCTCC NO: M2014108. The fermentation extract has obvious inhibitory effects on Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletotrichum gloeosporioides, Cylindrocladium scoparium or Curvularia lunata. Therefore, the invention provides scientific references for developing new anti-pathogenic fungus drugs and exploring deep-sea-fungus-derived natural active substances.

The invention relates to a method for biologically synthesizing crude extracts of theaflavins with cladosporium tenuissimum, which comprises following steps: obtaining high-producing strain cladosporium tenuissimum of polyphenol oxidase through screening a large amount of microbes, culturing the strains to synthesize polyphenol oxidase in optimized fermentation medium under the constant temperature and under the condition that the pH value is 4.5-5.6, wherein the rotation speed is 110-130r / min and the temperature is 22-25 DEG C., obtaining crude enzyme liquid of the polyphenol oxidase, and inputting oxygen to simulate oxidation catechin for 40-50 minutes in vitro to prepare the theaflavins. The theaflavins content which is higher than 20.00% in raw products of the theaflavins can be obtained through applying the method of the invention, the process steps are simple, the equipment investment is little, the working efficiency is high, the production cost is low, the method does not use any organic solution in the reaction process, and the method is a green environmental protection theaflavins producing method.

The invention provides an AchE (acetylcholinesterase) inhibiting secondary metabolite of endophytic cladosporium sp. Pt-2 of portunus trituberculatus and a preparation method thereof, belonging to the technical field of biology. The preparation method comprises the following steps of: (1) carrying out liquid fermentation on endophytic cladosporium sp. Pt-2 of portunus trituberculatus taken as a strain to obtain a fermented product; (2) carrying out vacuum concentration on fermentation liquor at 40 DEG C until the volume of the fermentation liquor is 1 / 3 of the original volume, then extracting the fermentation liquor with ethyl acetate and concentrating extract liquor until the extract liquor is dry; and (3) carrying out silica column chromatography on the organic phase extract, which has been concentrated until the extract is dry, of the cladosporium sp. Pt-2 fermentation product, carrying out gradient elution with a chloroform / methanol system to obtain six polarity segments and separating the segment with the highest activity by adopting a C18 reversed-phase column and sephadex chromatography, thus obtaining the secondary metabolite with AchE inhibition ratio up to 70-90%. The secondary metabolite and the preparation method have the advantages that the defects that current drugs are expensive and have side effects and high chemical synthesis difficulty are overcome; and the new drug and preparation method are provided to provide theoretical bases for development of senile dementia preventing biological drugs.