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Fungi with effect of increasing content of notoginsenoside and application of fungi

A technology of Panax notoginseng saponins and Panax notoginseng is applied to the biocontrol bacterium Cladosporium fungus and its application, the Cladosporium fungus and its application field in increasing the content of Panax notoginseng saponins, and can solve the problem of increasing the content of Panax notoginseng saponins, Increase the risk of environmental pollution and other issues to achieve the effect of increasing the content, reducing the quality of medicinal materials, and reducing agricultural pollution

Active Publication Date: 2018-11-16
SHENYANG PHARMA UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for promoting the growth of Panax notoginseng and improving the yield of medicinal materials due to unscientific application of chemical fertilizers, hormones, pesticides, etc. in the current production of Panax notoginseng, resulting in significant changes in the properties of medicinal materials and increasing the risk of environmental pollution. Cladosporium tenuissimum with increased content of notoginseng saponin and its application

Method used

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  • Fungi with effect of increasing content of notoginsenoside and application of fungi
  • Fungi with effect of increasing content of notoginsenoside and application of fungi
  • Fungi with effect of increasing content of notoginsenoside and application of fungi

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]The promoting action of embodiment 1 Cladosporium tenuissimum (Cladosporium tenuissimum) fermented liquid to Radix Panax notoginseng root system enzyme activity

[0031] Inoculate the described bacterial strain on a PDA medium plate, culture it upside down at 25-28°C for 5-8 days, activate the bacterial strain, prepare the activated bacterial strain into a spore suspension using sterile water, and count the spore concentration under a microscope. The concentration of the spore suspension is: 1.0×10 4 -1.0×10 8 individual / mL. Take the spore suspension and inoculate it in the fermentation medium PDB, culture it for 5-8 days at 25-28° C., 120-150 r / min, dilute the fermentation broth 100 times, and set aside. The biennial Panax notoginseng plants in the field were provided by the Institute of Medicinal Botany, Yunnan Academy of Agricultural Sciences. Using distilled water as a control, each group was replicated three times, and each replicate was inoculated with 100 Panax...

Embodiment 2

[0036] Example 2 Cladosporium tenuissimum (Cladosporium tenuissimum) fermented liquid extract promotes the agronomic index of Panax notoginseng

[0037] Inoculate the described bacterial strain on a PDA medium plate, culture it upside down at 25-28°C for 5-8 days, activate the bacterial strain, prepare the activated bacterial strain into a spore suspension using sterile water, and count the spore concentration under a microscope. The concentration of the spore suspension is: 1.0×10 4 -1.0×10 8 individual / mL. Inoculate the spore suspension in the fermentation medium PDB, culture at 25-28°C, 120-150r / min for 5-8 days, extract with equal volume of ethyl acetate three times, collect the concentrated extract, dilute 100 times, and set aside. The biennial Panax notoginseng plants in the field were provided by the Institute of Medicinal Botany, Yunnan Academy of Agricultural Sciences. Using distilled water as a control, each group was replicated three times, and each replicate was...

Embodiment 3

[0041] The effect of embodiment 3 Cladosporium tenuissimum (Cladosporium tenuissimum) fermented liquid on the content of notoginseng saponins

[0042] Inoculate the described bacterial strain on a PDA medium plate, culture it upside down at 25-28°C for 5-8 days, activate the bacterial strain, prepare the activated bacterial strain into a spore suspension using sterile water, and count the spore concentration under a microscope. The concentration of the spore suspension is: 1.0×10 4 -1.0×10 8 individual / mL. Take the spore suspension and inoculate it in the fermentation medium PDB, culture it for 5-8 days at 25-28° C., 120-150 r / min, dilute the fermentation broth 100 times, and set aside. The biennial Panax notoginseng plants in the field were provided by the Institute of Medicinal Botany, Yunnan Academy of Agricultural Sciences. Using distilled water as a control, each group was replicated three times, and each replicate was inoculated with 100 Panax notoginseng seedlings, a...

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Abstract

The invention relates to cladosporium tenuissimum as the fungi with the probiotic effect to panax notoginseng and the capability of increasing the content of notoginsenoside as well as an inoculant ofthe cladosporium tenuissimum and belongs to the technical field of biology. The bacterial strain is separated from stems of healthy biennial panax notoginseng, is authenticated to be the cladosporiumtenuissimum and is preserved in China General Microbiological Culture Collection Center with a preservation number being CGMCC No.14126. Preparation of the inoculant mainly comprises the following steps of pure culture of microorganisms, preparation of a spore suspension, production of the inoculant and application of the inoculant. The inoculant can also be used by being mixed with an organic fertilizer to prepare a bacterial fertilizer, has the probiotic effect to the panax notoginseng, can promote increase of the content of the notoginsenoside to a certain extent and shows a good ecological application prospect.

Description

Technical field: [0001] The present invention relates to a biocontrol fungus Cladosporium tenuissimum and its application, in particular to a Cladosporium tenuissimum with probiotic effect on the growth of Panax notoginseng and its application in increasing the saponin content of Panax notoginseng, belonging to the plant field of biotechnology. Background technique: [0002] Sanqi (Panax notoginseng (Burk.) F.H.Chen), also known as "Tianqi" and "Jinbuhuan", is a perennial medicinal and edible herb of Araliaceae. It was first recorded in "Compendium of Materia Medica". Partly used as medicine, it has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain. It is a traditional bulk commonly used medicinal material in the medicinal material market in my country. The market demand is large and the development and utilization value is high. Panax notoginseng mainly contains components such as saponins, flavonoids, notoginseng (amino...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P21/00A01G7/06C05F11/08C12R1/645
CPCA01G7/06A01N63/30C05F11/08C12N1/14C12N3/00C12N1/145C12R2001/645
Inventor 翟航章朦玥张怡轩张金渝杨天梅杨绍兵
Owner SHENYANG PHARMA UNIVERSITY
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