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Cladosporium sp. and application thereof in degradation of polyurethane plastic

A technology of Cladosporium and polyurethane, which is applied in the field of biological treatment, can solve the problems of lack of recycling, ecosystem damage, threat, etc., and achieve high degradation effect

Active Publication Date: 2021-03-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The widespread use of a large number of PU products has produced a lot of waste plastics, and there is a lack of reasonable recycling, which has caused serious damage and threats to the ecosystem.

Method used

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  • Cladosporium sp. and application thereof in degradation of polyurethane plastic
  • Cladosporium sp. and application thereof in degradation of polyurethane plastic
  • Cladosporium sp. and application thereof in degradation of polyurethane plastic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation, screening and strain identification of the highly efficient Impranil DLN strain Cladosporium sp.P7

[0033] Take 5g landfill soil samples and place them in 100mL enrichment medium containing 0.2% (v / v) Impranil DLN, and culture them at 30°C and 180rpm for 7 days. Measure the degradation situation to Impranil DLN in the enrichment solution with ultraviolet spectrophotometer, after confirming that Impranil DLN is degraded, insert with the inoculation amount of 10% (v / v) to contain 0.4% (v / v) Impranil DLN rich Collect in the culture medium, continue to enrich and measure the degradation situation, follow this method until the concentration of Impranil DLN increases to 1% (v / v), and then pass for 4 times.

[0034] The Impranil DLN enrichment solution was serially diluted and spread on an inorganic salt plate with 1% (v / v) Impranil DLN as the sole carbon source, and cultured in a 30°C incubator for 1-7 days. Single colonies with different colony shapes...

Embodiment 2

[0038] Example 2: The degradation curve of Cladosporium sp.P7 to Impranil DLN and the change of esterase activity during the degradation process

[0039] (1) The making of different concentrations of Impranil DLN standard music: respectively configure the Impranil DLN inorganic salt culture medium with concentrations of 0.2, 0.4, 0.6, 0.8, 1.0% (v / v) (wherein 1% v / v is equal to 6g / L DLN), respectively in OD 600nm Determination, drawing a standard curve, such as figure 1 shown.

[0040] The calculation of DLN degradation rate is published as follows:

[0041] DLN degradation rate%=(initial concentration-final concentration) / initial concentration*100%

[0042] (2) Wash the P7 mycelium from the PDA flat plate with sterile water, scrape the mycelium and spores on the surface with a coating stick, count through the hemocytometer, and make a final concentration of 1 × 10 6 The bacterial suspension with spore / mL concentration is the seed solution.

[0043] (3) In the inorganic s...

Embodiment 3

[0045] Embodiment 3: Research on the degradation performance of Impranil DLN by Cladosporium sp.P7

[0046] Will 1×10 6 The seed solution of spore / mL was inserted into the inorganic salt medium with the final concentration of Impranil DLN of 1% (v / v) at 10% (v / v) inoculum amount, respectively at 20, 25, 30, 37 and 42 °C, 120rpm shaker cultured for 7 days, detected the residual amount of Impranil DLN, calculated the degradation rate, and studied the effect of temperature on the degradation of Impranil DLN. Such as Figure 5 As shown in a, the optimal degradation temperature of strain P7 is 30°C, at this time the degradation rate of Impranil DLN is the highest, and the degradation rate is 97%; when the temperature is 25°C and 37°C, the degradation percentages of strain P7 are 88% and 77%; when the temperature is lower (20°C) or higher (42°C), the degradation rate of strain P7 to Impranil DLN is lower than 40%. This shows that low temperature is not conducive to the degradatio...

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Abstract

The invention discloses a cladosporium sp. and application thereof in degradation of polyurethane plastics. The cladosporium sp. is classified and named as Cladosporium sp., the strain name is P7, thecladosporium sp. is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the strain is CCTCC NO: M 2020627, and the preservation date is October 23, 2020. The strain P7 adopted by the invention can grow by taking Impranil DLN as a unique carbon source, and the degradation rate of the waterborne polyurethane Impranil DLN reaches 95.8% within 7 days. In addition, the strain has a relatively high degradation effect on polyurethane foam, and 86.6% of polyurethane foam can be degraded within 15 days. Therefore, the strain provided by the invention has an extremely important application value in biological treatment of plastic wastes.

Description

technical field [0001] The invention belongs to the technical field of biological treatment, and relates to a strain of Cladosporium and its application in degrading polyurethane plastics. Background technique [0002] Polyurethane (PU) is a polymer containing urethane bond repeating unit structure formed by the condensation of three components: polyisocyanate, polyol and chain extender. It is a semi-crystalline thermosetting plastic, in which isocyanate is the hard segment that constitutes the crystalline part, called PU, which determines its hardness and tensile strength; while polyol and chain extender are the soft segment that constitutes the non-crystalline part, called PU , determines its elasticity and extension properties. By changing the types and ratios of polyols and isocyanates, PUs with various properties can be produced. Therefore, PU products are more diversified than other plastics, and due to their excellent mechanical properties, PU plastics are widely us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C08J11/10C08L75/04C12R1/645
CPCC12N1/14C08J11/105C08J2375/04Y02W30/62
Inventor 董维亮刘嘉唯姜岷周杰许斌徐安明刘云龙
Owner NANJING UNIV OF TECH
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