75 results about "Theaflavine" patented technology

A method and composition for preventing and treating Avian Influenza in Humans utilizes an effective quantity of polyphenolic(s) and / or its derivatives in combination with a carrier. The anti-avian influenza ingredient having a composition selected from the group consisting of theaflavin, theaflavin-3,3′-digallate, theaflavin-3-monogallate, theaflavin-3 gallate, theaflavin-3′-gallate, thearubigin, gallic acid, tannic acid, (−)-epigallocatechin gallate (EGCG), (−) epigalloatechin (EGC), (+)-epicatechin (EC), (−)-gallocatechin gallate (GCG), and catechin.

The invention provides a new process for using the enzyme owned by tea to converting tea polyphenol in the tea into theaflavin through biotransformation. The preparation process is as follows: adding fermenting enzyme made from fresh tea into green tea extracted by ethyl acetate, carrying out stirring and fermentation under the condition of pure oxygen, then going through separation, concentration, washing, conversion dissolving and filtration, and at last carrying out vacuum freeze drying, crushing and screening to obtain qualified theaflavin product. The theaflavin product made through the process has a stable quality, high theaflavin content, high yield and low cost. In addition, the method adopts pure ester phase oxidative fermentation, thereby solving the problem that the three phases are incapable of being separated after the traditional ester, water and refuse mixed-phase fermentation, and consequently carrying out stable and controllable industrialized production.

A method for preparing tea color matter with bacterial liquid fermentation, belonging to tea production field. It comprises the following steps: a. disintegrating the tea into 60-80 mesh, adding distilled water according to proportion 1-10g: 100-200 ml, sterilizing under 120 Deg C for 10 minutes, inoculating bacterial with 0.1g per 100 ml distilled water; b. fermenting on shaking table under 25-30 Deg C for 15 days, the rotation rate of shaking table is 110 r per minute; c. drawing out 100 ml fermentation liquor, adding 100 ml acetic ether, shaking in rating funnel of 500 ml for 10 minutes for stratification, putting acetic ether on upper layer and water on lower layer onto triangle bottle of 200 ml; d. sucking out acetic ether tobacco extract 50 ml, adding NaHCO3 solution of 2.5% 50 ml, vibrating 30 seconds under rotating rate being 300 r per min for stratification, evaporating acetic ether to dryness and getting theaflavine; e. evaporating water to dryness and getting tea red element; f. sucking out 50 ml fermentation liquid and 50 ml butyl alcohol into rating funnel, vibrating 5 minutes, evaporating lower layer to dryness and getting tea brown element. The invention is characterized by the controllable production condition and environment and free from production season limit.

The invention discloses a nutritional and healthcare yam beverage and a preparation method. 1000 parts of yam beverage is prepared from the following raw materials in parts by weight: 25-60 parts of yam, 2-50 parts of pear, 20-80 parts of tea polyphenol, 683-901 parts of water, 15-40 parts of chrysanthemum, 1-7 parts of stabilizer, 16-65 parts of sweetening agent and 2-15 parts of sour agent. The production method comprises the steps as follows: preprocessing the raw materials; pulping; mixing; carrying out enzyme reaction; blending; homogenizing; filling; sterilizing; and checking. The yam beverage is sour, sweet, tasty, unique in flavor, rich in nutrition and rich in theaflavin, and has the effects of lowering blood fat and preventing cardiovascular and cerebrovascular diseases.

The present invention discloses process of separating black tea extract to prepare four kinds of high purity theaflavin and its derivatives in batch. By means of combining gel column chromatographic separation with great treating quantity and high speed countercurrent chromatographic separation with high separating efficiency, four kinds of theaflavin and its derivatives, including theaflavin (TF), theaflavin-3-gallate (TF-3-MG),theaflavin-3'-gallate (TF-3'-MG) and theaflavin-3, 3'-gallate (TFDG), in high purity are prepared. The process has great preparation amount, high product purity, short period, low solvent consumption and other features.

The invention relates to the plant extraction technical field, in particular to a theaflavin extraction and purification method, which overcomes the defects of low product purity, high cost, low yield, small sample size and long separation time in theaflavin extraction in the prior art. The method comprises the following steps: (1) fermentation: mixing green tea with polyphenol oxidase based on the mass ratio of 100:0.01-0.1 for oxidative fermentation at the temperature of 20-80 DEG C for 1-12h; (2) extraction: transferring the fermented green tea into continuous countercurrent equipment for extraction at the temperature of 40-80 DEG C for 0.5-3h to obtain extracting solution for later use; and (3) purification: purifying with macroporous adsorbing resin, and performing gradient elution with ethanol and water. The method of the invention can help greatly save production time, effectively lower production cost and improve theaflavin purity, thus meeting the need of scientific research and production.

The invention discloses a tea oral tablet and a preparation method thereof. The tea oral tablet uses theaflavin and theine-removing tea polyphenol as main raw materials, and filling agent, flavouring agent, bonding agent and disintegrant are added into the main raw materials to prepare the oral tablet product. The product has the physiologic function of the theaflavin and the tea polyphenol, is favorable to the oxidation resistance of a human body to remove free radicals for keeping the function of the human body and is easy to take and convenient to carry.

The present invention relates to a process for producing a black tea product with enhanced sensorials. The people who prefer to consume black tea are unable to get a good amount of catechins, because, in black tea the amount of catechins is significantly less than that of green tea. Therefore there is a need for providing a tea product which has relatively high amount and catechins high amount of theaflavins and high amount of polyphenols made by a process without addition of any exogenous theaflavins and / or catechins and which also has the sensorials of black tea. It is an object of the present invention to provide a process for producing a tea product with a relatively high amount of catechins and theaflavins without the addition of any exogenous theaflavins and / or catechins. The present inventors have surprisingly found that tea products obtained by a process involving a step of anaerobic incubation at specific temperatures and for specific durations provide a tea product with improved sensorials and thereby satisfying one or more of the objects of the invention.

The invention provides a method for preparing theaflavin by recycling polyphenol oxidase. The method comprises the following steps: 1) adding a polyphenol oxidase liquid and a tea liquid into a fermentation liquid; (2) introducing oxygen according to the amount of 0.5-2 units per minute, and performing fermentation for 40-150 minutes at a temperature of 20-40 DEG C at a rotating speed of 60-150 r / min; 3) performing filtration in an inert gas environment at a fermentation liquid temperature of 20-35 DEG C; and 4) purifying, concentrating and drying permeate to obtain theaflavin, wherein the polyphenol oxidase liquid returns to the fermentation liquid in the step (1) to participate in fermentation, so that the polyphenol oxidase liquid is recycled and the high-purity theaflavin is obtained.The polyphenol oxidase liquid is recycled, the activity of polyphenol oxidase is maintained, waste of polyphenol oxidase is avoided, the production cost of the theaflavin is greatly reduced, continuous production is facilitated, and safety and reliability are achieved.

The invention discloses a novel alpha-glucosidase active inhibitor, belonging to the field of functional factors of functional foods and medicines; tea pigments in the invention can be used for strongly inhibiting alpha-glucosidase activity; the IC50 of alcohol-soluble theaflavin, the IC50 of thearubigin and the IC50 of theabrownin are 3.94 mu g / ml, 3.24 mu g / ml and 75.43 mu g / ml respectively; the half inhibition rate IC 50 of alcohol-soluble theaflavin, the half inhibition rate IC50 of thearubigin and the half inhibition rate IC50 of theabrownin are 8.19 mu g / ml, 4.50 mu g / ml and 6.98 mu g / ml respectively; the alpha-glucosidase inhibition effect of alcohol-soluble tea pigments or water-soluble tea pigments is far prior to that of the common hypoglycemic medicine, namely acarbose (acarbose IC50=1.04 mg / ml); and therefore, the novel alpha-glucosidase active inhibitor can be used as a food additive or a medicine for treating diabetes. Because of being pure natural pigments extracted from tea leaves, the tea pigments are safe, nontoxic and better in blood sugar decreasing effect; the advantages of the tea pigments are imponderable by acarbose; and therefore, the novel alpha-glucosidase active inhibitor has the application prospect for being developed into a health-care product or a novel hypoglycaemic medicine.

The invention provides a method for preparing theaflavin. The method is characterized by comprising the following steps that (1) a polyphenol oxidase emulsion is prepared; (2) a tea polyphenol alcoholic solution is prepared; (3) the polyphenol oxidase emulsion and the tea polyphenol alcoholic solution are evenly mixed according to the mass ratio of 1:(0.5 to 2.0), the pH value is adjusted to be 4.0-6.5, air or oxygen is introduced at 25-35 DEG C with the amount of 0.5-2 units / min, and fermentation is conducted for 20-80 min; (4) still standing is conducted for 2-5 h, supernatant is taken, filtered, concentrated and diluted with purified water till a solid type object is 3%-6%; and (5) chromatography, elution, concentration and drying are conducted. The fermentation synthesis rate of the theaflavin is high, purification is easy, residue is avoided, safety and reliability are achieved, a technology is simple, and the production cost of the theaflavin is significantly lowered.

Disclosed is a composition or beverage / food for inhibiting the fat absorption, which is derived from a natural material and therefore is safe, and which can be ingested continuously. The composition or beverage / food comprises the following components: (A) theaflavine monogallate; (B) theaflavine; (C) theaflavine digallate; and (D) polyphenol, wherein these components satisfy the following requirements: (1) a weight-based content ratio [(A) / (B)] is 0.4-100%; (2) the weight of the component (A) is larger that that of the component (C); and (3) a weight-based content ratio [A) / (D)] is 0.01-1.0%. The composition or beverage / food can be used for the prevention or treatment of a disease such as arteriosclerosis and an ischemic heart disease (e.g., angina pectoris, myocardial infarction).

The invention relates to a method for preparing flavored black tea. The method comprises the following steps: (1) selecting varieties of foreign aid enzymes and determining a compounding ratio; (2) sprinkling a mixed-enzyme solution during withering of tea leaves; (3) continuing to sprinkle the mixed-enzyme solution during rolling of the tea leaves; (4) fermenting the tea leaves; and (5) finally drying the fermented black tea leaves, thereby obtaining a finished product. According to the method disclosed by the invention, the black tea is produced by employing mixed fermentation of alkaline proteinase and papain, proteins in the tea leaves are hydrolyzed into micromolecular peptides and amino acids by these foreign aid enzymes at appropriate temperatures, and meanwhile, the condition thattea polyphenols are converted into thearubigin and theaflavin by polyphenol oxidase is not cut off, so that the content of nutrient amino acids in tea soup can be effectively increased, and the quality of the black tea is improved; and meanwhile, L-cysteine is added to prevent the tea leaves from excessive fermentation, so that the prepared black tea is better in mouth feel and good in soup color.

The invention belongs to the technical field of oral cavity bonding, and particularly relates to an oral cavity acid etching agent and a preparation method and application thereof. The oral cavity acid etching agent comprises theaflavin and glycolic acid. According to the oral cavity acid etching agent provided by the invention, the theaflavin is added, so that the strength of demineralized dentin collagen fibers is enhanced, the MMPs activity is inhibited, the process of caries is blocked, and meanwhile, the durability of a dental resin filling body is improved.

The invention relates to a preparation method of long-term antibacterial and fresh edible coating liquid, and belongs to the technical field of food processing. TP (tea polyphenol) extract liquid is taken as a raw material, by adding casein and theaflavin extracts, the long-term antibacterial and fresh edible coating liquid is prepared, TP is subjected to a complexation reaction with proteins in casein, precipitation will be formed under the action of hydrophobic-hydrogen bonding, with degree of association increasing, complexes show colloidal properties, and the solubility of the complexes will be changed with temperature; furthermore, the proteins are precipitated after phenolic hydroxyl groups interacting with the proteins and amino acids, so that the TP has the effects of sterilization, antivirus and the like. The complexation reaction is invertible. Curcumin has the double activity of oxidation loving and anti-oxidation, and has certain inhibitory effect on most of the bacteria and the inhibitory effect is significant, which is a good natural antibacterial agent and can effectively improve antibacterial energy of the edible coating liquid and preservation time of food.

The invention provides a composition capable of lowering uric acid and a preparation method thereof, and relates to the field of health food. The main functional components of the composition includetheaflavin, resveratrol, dihydromyricetin, theanine and cordycepin in set proportion. The composition mainly plays a role to lower the uric acid and resist to gout by inhibiting the activity of xanthine oxidase, adjusting related anionic transporter mRNA and protein expression levels and promoting uric acid excretion; the composition can also relieve the matrix degradation of chondrocyte after IL-1[beta] stimulation and reduce the expression of inflammatory factors, so that the symptoms of osteoarthritis and bone and joint pain can be relieved; and the composition is significant in efficacy, obvious in uric acid lowering, good in safety and convenient in taking, and has good development and application prospects.

The invention is applicable to the technical field of medicines, and provides a theaflavin composition for promoting wound healing as well as a preparation method and application thereof. The main component of the theaflavin composition for promoting wound healing is a theaflavin-3-3 '-digallate monomer or a theaflavin crude product; when the main component of the theaflavin composition is theaflavin-3-3 '-digallate monomer, the theaflavin composition further comprises a component A or a component B, or the theaflavin composition simultaneously comprises the component A and the component B; when the main component of the theaflavin composition is a theaflavin crude product, the theaflavin composition also comprises a component B; the theaflavin composition has the advantages of improving the stability of medicine molecules, increasing the bioavailability of the medicine molecules, improving poor dissolution, low treatment efficiency and the like of traditional medicines, can effectively promote synthesis of wound collagen, reduces infiltration of inflammatory cells, accelerates differentiation of newborn cells and formation of blood vessels, obviously promote the wound healing of diabetic mice, and the application value of potential wound healing is great.

The invention belongs to the technical field of biomedicine, and in particular relates to the application of theaflavin-3,3'-digallate in the preparation of anti-inflammatory drugs. The present invention uses two inflammatory cell models to demonstrate for the first time the anti-inflammatory activity of the small molecule monomer compound TF-3; the present invention establishes a mouse acute lung injury inflammation model for the first time to prove that TF-3 inhibits the prevention and treatment of lung injury inflammation in vivo Applications. The experimental results prove that: TF‑3 can effectively inhibit the expression of inflammatory factors TNF‑α, IL‑1β and IL‑6 genes, and can inhibit the production of inflammatory cytokines in the serum of mice with acute lung injury, and can prevent the infiltration of inflammatory cells, Treat lung damage. The medium and small molecule monomer compound theaflavin-3,3'-digallate body of the present invention has low toxicity and remarkable anti-inflammatory effect, and overcomes the shortcomings of existing anti-inflammatory drugs such as high toxicity and easy drug resistance. question.

The invention relates to a natural extract composition having an oxidation resistant effect, a preparation and an application. The natural extract composition comprises the following components in parts by weight of 0.5-3 parts of theaflavin, 0.5-2 parts of astaxanthin, 0.5-2 parts of a grape seed extract and 0.5-2 parts of a grape skin extract. The preparation contains the natural extract composition. The invention further provides the application of the natural extract composition for preparing medicines having the oxidation resistant effect. The theaflavin regulates a bioactivity enzyme system, so that the production of free radicals can be directly or indirectly removed or restrained, and lipid peroxidation can be restrained; the astaxanthin quenches singlet oxygen and eliminate free radicals, so that membrane permeability can be reduced, an oxidizing agent can be limited from permeating into cells, and the defending effect of an endogenous antioxidative enzyme system can be improved; proanthocyanidins can effectively eliminate superoxide anion free radicals and hydroxyl radicals; and resveratrol can remove or restrain the generation of the free radicals, restrain lipid peroxidation and regulate enzymic activity relevant to oxidation resistance.

The invention discloses a method for separating high-purity theaflavin from black tea pigment solution. The black tea pigment solution obtained by extracting black tea or oxidation of tea polyphenols is used as raw material, and AB-8 or NKA-9 or Mitsubishisp-207 and other large Porous resin is used as a separation medium, and ethanol aqueous solution is used as an eluting solution to separate and obtain high-purity theaflavin; the method of the invention has simple process, low energy consumption, simple and safe operation, and is beneficial to realizing large-scale continuous production.

The invention belongs to the technical field of veterinary drugs, and discloses an application of theaflavin in preparation of a drug for resisting avian pathogenic bacteria. The applicant discovers that theaflavin has the effects of inhibiting the growth of duck-origin chicken bacilli and avian pasteurella and killing the duck-origin chicken bacilli and avian pasteurella, the minimum inhibitory concentration of theaflavin to the duck-origin chicken bacilli is 128 mu g / ml, the minimum bactericidal concentration of theaflavin to the duck-origin chicken bacilli is 512 mu g / ml, the minimum inhibitory concentration of theaflavin to the avian pasteurella is 512 mu g / ml, and the minimum bactericidal concentration of theaflavin to the avian pasteurella is 512 mu g / ml. The toxic and side effects of vaccines, chemical drugs and antibiotics and potential food safety hazards are overcome, and wide application prospects and development values are achieved in the fields of food processing, medicine and breeding industry.

The invention belongs to the technical field of enzyme engineering, and relates to a tyrosinase mutant and application thereof. According to the tyrosinase mutant, a plurality of amino acid sites are mutated in wild type tyrosinase with an amino acid sequence as shown in SEQ ID NO.1. According to the tyrosinase mutant and the method for preparing theaflavin by using the tyrosinase mutant, the mutant has higher specific activity than wild type tyrosinase, and has higher yield during catalytic preparation of theaflavin.

A black tea extract, comprising 20-30% parts by weight of theaflavin and 40-50% parts by weight of catechin. The theaflavin and the catechin in the black tea extract can be obtained by conventional high-concentration tea polyphenol extraction in the art, and can also be commercially available theaflavin and catechin in the art.

The invention relates to a thymosin enteric-coated tablet and a preparation method thereof, in particular to a composite thymosin enteric-coated tablet and a preparation method thereof, and aims to solve the problems of low release rate and high drug cost of existing thymosin preparations. The enteric-coated tablet comprises a drug-carrying microsphere, polyethylene glycol 3350, microcrystalline cellulose and magnesium stearate. The drug-carrying microsphere is made of thymosin, theaflavin and an enteric polymer auxiliary material. The preparation method includes 1, mixing the thymosin with the theaflavin and the enteric polymer auxiliary material to obtain a mixture, and adding the mixture into cosolvent to dissolve to obtain co-dissolved solution; 2, adopting a high-voltage static sprayer to spray the co-dissolved solution obtained in the step 1 to a powder reception plate to finally obtain the drug-carrying microsphere; 3, evenly mixing the drug-carrying microsphere with the microcrystalline cellulose, the polyethylene glycol 3350 and the magnesium stearate and tableting the mixture to obtain the thymosin enteric-coated tablet. The thymosin enteric-coated tablet and the preparation method thereof have the advantages that thymosin immunity can be enhanced through composition of the theaflavin and the thymosin, dosage of thymosin bulk drugs is reduced, and release rate of the enteric-coated tablet is high.

The invention relates to a method for reducing the residual amount of nitrite in sausages. The method is characterized by comprising the following steps: adding 0.01-0.04 part of theaflavin into meatpaste in the sausage making process, stirring to sufficiently mix the theaflavin with the meat paste, and performing pickling and filling. When the sausage disclosed by the invention is prepared, thetheaflavin is added during meat paste pickling, so that the residual amount of nitrite in the pork sausage can be reduced; the effect of improving the color quality of the pork sausages is also achieved. When 0.03 part of the theaflavin is added into every 100 parts of mixed meat paste, the nitrite content in the pork sausage reaches the minimum value, and the redness value is the maximum.

The invention relates to a method for biologically synthesizing crude extracts of theaflavins with cladosporium tenuissimum, which comprises following steps: obtaining high-producing strain cladosporium tenuissimum of polyphenol oxidase through screening a large amount of microbes, culturing the strains to synthesize polyphenol oxidase in optimized fermentation medium under the constant temperature and under the condition that the pH value is 4.5-5.6, wherein the rotation speed is 110-130r / min and the temperature is 22-25 DEG C., obtaining crude enzyme liquid of the polyphenol oxidase, and inputting oxygen to simulate oxidation catechin for 40-50 minutes in vitro to prepare the theaflavins. The theaflavins content which is higher than 20.00% in raw products of the theaflavins can be obtained through applying the method of the invention, the process steps are simple, the equipment investment is little, the working efficiency is high, the production cost is low, the method does not use any organic solution in the reaction process, and the method is a green environmental protection theaflavins producing method.

The invention relates to a female private gel microecological preparation composition and a preparation method thereof. The coating comprises the following components in percentage by mass: 3.0-5.0% of glycerin, 5.0-10.0% of butanediol, 3.0-5.0% of poloxamer, and 2.0-4.0% of lactobacillus rhamnosus fermentation product filtrate, 1.0-2.0% of theaflavin extracting solution, 1.0-2.0% of bamboo fungusindusium extracting solution, 0.3-0.8% of lactic acid, 0.2-0.4% of allantoin, 0.1-0.2% of EDTA disodium, 0.0001-0.1% of carbonized polyamine nanoparticle solution, and 84.3999-70.5% of deionized water. The composition has a better antibacterial effect, small irritation to vaginal skin and high safety performance, and has the effects of moisturizing, fading and improving vaginal lip pigment, improving vaginal pruritus, improving vaginal peculiar smell and the like.

The invention relates to an anti-saccharification whitening theaflavin composition and application thereof, and the anti-saccharification whitening theaflavin composition comprises the following components in parts by weight: 5-10 parts of elastin, 5-10 parts of theaflavin, 5-10 parts of olive fruit powder, 0.1-0.2 part of nicotinic acid or nicotinamide, 100-200 parts of collagen tripeptide and 3-8 parts of vitamin C. According to the anti-saccharification whitening theaflavin composition disclosed by the invention, all the components have a synergistic enhancement effect, and the anti-saccharification whitening theaflavin composition is added into a health-care product, is free of sugar, is easily absorbed by a human body, and can improve the problems of skin yellowing and dark color caused by saccharification, oxidation and melanin.

A process for producing a purified extract comprising between about 40% and about 90% theaflavins is provided. Purified theaflavin extract produced by the disclosed process is provided which comprises less than about 5% TF1, between about 10% and about 60% TF2a, between about 5% and about 35% TF2b, and between about 10% and about 65% TF3. Individual dosage compositions are provided for the control of a physiological disorder comprising about 5% to about 95% theaflavins in a pharmaceutically acceptable vehicle or a dietary supplement vehicle. Further individual dosage compositions are provided which comprise an effective amount of substantially only one theaflavin species selected from the group consisting of TF1, TF2a, TF2b, and TF3. Individual dosage compositions are provided which comprise an effective amount of substantially only two theaflavin species selected from the group consisting of TF1 and TF2a, TF1 and TF2b, TF1 and TF3, TF2a and TF2b, TF2a and TF3, TF2b and TF3. Methods of treatment of human physiological disorders are provided which comprise administering oral dosage forms of the compositions.

The invention discloses a method for preparing theaflavin through enzyme catalysis and a product prepared by the method.The method comprises the steps: step S1, crushing fresh tea leaves, and adding petroleum ether or ethyl acetate and performing uniform stirring to obtain a mixture; step S2, removing the petroleum ether or ethyl acetate in the mixture by filtering to obtain leaf powder; step S3,adding a tea polyphenol product and water into the leaf powder, and adding ethyl acetate or butyl acetate to obtain a mixed system; step S4, carrying out aerobic fermentation on the mixed system; andstep S5, taking fermentation broth, and performing drying to obtain theaflavin solids. The petroleum ether or ethyl acetate is added into the fresh tea powder to remove caffeine, theophylline wax, chlorophyll and non-volatile grease so as to prepare the leaf powder containing a polyphenol oxidase enzyme system, and the tea polyphenol product is catalyzed by taking the leaf powder as an immobilizedenzyme catalyst to ferment to prepare the theaflavin; and the natural cured polyphenol oxidase enzyme system is reserved in the leaf powder, and the influence on the enzyme activity is small.