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New process for preparing theaflavine

A technology of theaflavin and a new process, applied in the field of preparing theaflavin, can solve the problems of high content of theaflavin, inability to separate three phases, inability to filter, etc., to achieve increased yield and content, easy control of the fermentation process, and elimination of the The effect of waste water

Active Publication Date: 2008-10-15
JIANGSU DEHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a new process for preparing theaflavins against the shortcomings in the above-mentioned process for preparing theaflavins. , the active substance tea polyphenols in tea leaves are aerobically fermented into theaflavins. The product is green and environmentally friendly, with stable quality and high theaflavin content. 2. High yield and low cost. 3. No strong acid or alkali is used in the production process, and no Process wastewater is generated, which completely solves the environmental protection problems in the production process. 4. Due to the pure ester phase oxidation fermentation adopted in this method, the problem that the three phases cannot be separated after the traditional mixed phase fermentation of ester, water and slag is solved (often unable to filter and emulsification problems), so that stable and controllable process production can be carried out

Method used

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  • New process for preparing theaflavine

Examples

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Effect test

Embodiment 1

[0031] Embodiment 1: as figure 1As shown, a new process for preparing theaflavins, the process steps are as follows: put 100g of green tea into the extractor, then add 1000mL of ethyl acetate, control the temperature at 25-60°C, stir and extract for 30min, discharge the material, and then add Ethyl acetate 1000mL was extracted again in the same way, the extracts were combined, the above extracts were concentrated to a solid content of 1%, and cooled to room temperature as a fermentation stock solution; 120g of fresh fresh tea leaves were selected as raw materials for the enzyme, and the fresh tea leaves were used Wet it with pure water or 0.1% citric acid aqueous solution, place it below 0°C, freeze it for more than 2 hours, and immediately pulverize it after taking it out. The pulverization is carried out below room temperature, and the fresh tea leaves after pulverization are controlled to be above 80 mesh. After the treatment, the enzyme is used as a fermentation enzyme for...

Embodiment 2

[0032] Embodiment 2: as figure 1 As shown, a new process for preparing theaflavins, the process steps are as follows: put 100g of green tea into the extractor, then add 1500mL of ethyl acetate, control the temperature at 25°C-60°C, stir and extract for 30min, discharge, and then Add 1500 mL of ethyl acetate and extract again in the same way, combine the extracts, concentrate the above extracts to a solid content of 0.8%, cool to room temperature as a fermentation stock solution; select 180 g of fresh fresh tea leaves as raw materials for the enzyme, and fresh tea leaves Moisten it with pure water or 0.5% citric acid aqueous solution, place it below 0°C, freeze it for more than 2 hours, take it out and grind it immediately, and grind it below room temperature, and control the fresh tea leaves after grinding to 80 mesh or more , the treated enzyme is used as a fermented enzyme for later use (the storage time at room temperature shall not exceed 2 hours); 1500 g of the above-ment...

Embodiment 3

[0033] Embodiment 3: as figure 1 As shown, a new process for preparing theaflavins, the process steps are as follows: put 100g of green tea into the extractor, then add 3000mL of ethyl acetate, control the temperature at 25-60°C, stir and extract for 30min, discharge the material, and then add Ethyl acetate 3000mL is extracted once again in the same way, the extracts are combined, the above extracts are concentrated to a solid content of 0.5%, and cooled to room temperature as a fermentation stock solution; select fresh fresh tea leaves 180g as raw materials for the enzyme, and use fresh tea leaves Wet it with pure water or 1% citric acid aqueous solution, place it below 0°C, freeze it for more than 2 hours, and immediately grind it after taking it out. The grinding should be carried out below room temperature, and the fresh tea leaves after grinding should be controlled to be above 80 mesh After the treatment, the enzyme is used as a fermentation enzyme for later use (the sto...

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Abstract

The invention provides a new process for using the enzyme owned by tea to converting tea polyphenol in the tea into theaflavin through biotransformation. The preparation process is as follows: adding fermenting enzyme made from fresh tea into green tea extracted by ethyl acetate, carrying out stirring and fermentation under the condition of pure oxygen, then going through separation, concentration, washing, conversion dissolving and filtration, and at last carrying out vacuum freeze drying, crushing and screening to obtain qualified theaflavin product. The theaflavin product made through the process has a stable quality, high theaflavin content, high yield and low cost. In addition, the method adopts pure ester phase oxidative fermentation, thereby solving the problem that the three phases are incapable of being separated after the traditional ester, water and refuse mixed-phase fermentation, and consequently carrying out stable and controllable industrialized production.

Description

technical field [0001] The invention relates to a new process for preparing theaflavins, in particular to a new process for preparing theaflavins by bioconverting tea polyphenols in tea leaves with enzymes of tea itself. Background technique [0002] Cardiovascular and cerebrovascular diseases are serious diseases that endanger human health. According to the data of modern medical disease monitoring, nearly 3,600 people die from cardiovascular and cerebrovascular diseases in the world every day. Myocardial infarction, cerebral infarction, The number of people suffering from stroke is increasing at an annual rate of 12%. Cardiovascular and cerebrovascular diseases have become the number one killer of human health, and people are paying more and more attention to its prevention and treatment. Studies have shown that theaflavins have the best preventive and therapeutic effects on these diseases. [0003] Theaflavin is a natural pigment of large aromatic rings formed by ferment...

Claims

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Application Information

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IPC IPC(8): C12P17/16
Inventor 曹后建
Owner JIANGSU DEHE BIOTECH
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