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A saline-alkali-tolerant Bacillus megaterium fjw1 and its application in the preparation of phytopathogenic bacteriostatic agents

A technology of Bacillus megaterium and plant pathogenic bacteria, applied in the field of microorganisms, can solve problems such as limiting the scope of application, and achieve good antibacterial effect, rapid growth, and strong environmental adaptability

Active Publication Date: 2021-09-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported Bacillus megaterium that can be used as a biocontrol bacterium has only one or two types of inhibitory bacteria, which seriously limits its application range.

Method used

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  • A saline-alkali-tolerant Bacillus megaterium fjw1 and its application in the preparation of phytopathogenic bacteriostatic agents
  • A saline-alkali-tolerant Bacillus megaterium fjw1 and its application in the preparation of phytopathogenic bacteriostatic agents
  • A saline-alkali-tolerant Bacillus megaterium fjw1 and its application in the preparation of phytopathogenic bacteriostatic agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Isolation and identification of Bacillus megaterium FJW1:

[0048] Put 2.5g of soil samples from Pucheng County, Weinan City, Shaanxi into 50ml of sterile water, shake at 250rpm at 30°C for 1h, then take 500μl of soil suspension and carry out gradient dilution in a PA bottle filled with 4.5ml of sterile water. take 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 The soil suspensions of these dilutions were added to the culture medium for coating, and after 24h, 48h, 72h, and 96h of culture, the growth of plate colonies was observed, and all grown colonies were picked out for antibacterial activity experiments; Bacteria with high bacteriostatic rate and broad bacteriostatic spectrum were selected, and then further screened for saline-alkali tolerance; finally, Bacillus megaterium FJW1 was screened out.

[0049] (1) Morphological characteristics

[0050] The isolated saline-alkali-tolerant giant bacillus (Bacillus magaterium) FJW1, the colony morphology on LB solid medium is as f...

Embodiment 2

[0059] Tolerance test of Bacillus megaterium FJW1:

[0060] (1) pH range test:

[0061] The seed solution of Bacillus megaterium was inoculated into LB medium with different initial pH according to the inoculum amount of 1%, and cultured at 37°C and 200r / min. After 14 hours of culture, samples were taken to measure OD 600 . The initial pH gradient of the medium was set at 3, 4, 5, 6, 7, 8, 9, 10.

[0062] LB medium: peptone 10g, yeast extract powder 5g, NaCl 10g, distilled water 1000mL, pH 7.0, sterilized by high pressure steam at 121°C for 20min.

[0063] pH 3 4 5 6 7 8 9 10 OD 600

0.043 0.052 0.139 4.361 5.764 4.415 0.19 0.016

[0064] (2) Salt tolerance test:

[0065] The seed solution of Bacillus megaterium was inoculated into LB medium with different salt concentrations according to the amount of 1%, cultured at 37°C and 200r / min, and after 14 hours of culture, samples were taken to measure OD 600 . The salt concentration gradi...

Embodiment 3

[0072] Inhibitory effect of Bacillus megaterium FJW1 on plant pathogens:

[0073] The inhibitory ability of Bacillus megaterium to pathogenic bacteria was determined by zone of inhibition method. The activated Bacillus megaterium seed solution was appropriately diluted and spread on a PDA plate, and the sterile water was used as a control group. Punch holes on the PDA plate covered with pathogenic fungi, pick the bacterial block and inoculate it upside down on the PDA plate coated with Bacillus megaterium, make the side with the fungus touch the plate, and culture the plate at 37°C for 5 days. Then measure the fungal colony diameter, calculate the bacteriostatic rate, and then analyze the bacteriostatic effect.

[0074] The plant pathogens selected in this experiment are Fusarium oxysporum F.sp.Cubense, Fusarium oxysporum, Fusarium solani, Fusarium solani, Fusarium chlamydia Fusarium chlamydosporum, Penicillium glaucum, Rhizomucor variabilis, Banana wilt, Rhizopus nigricans,...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a strain of Bacillus megaterium FJW1 and its application in the preparation of bacteriostatic agents for plant pathogenic bacteria. The Bacillus megaterium FJW1 involved in the present invention has a preservation number of CCTCC M 2019660. The strain has alkali resistance, Strong environmental adaptability, anti-plant pathogenic bacteria and other functions; can grow in pH6‑pH8, 13% salt concentration, and has a good inhibitory effect on a variety of Fusarium pathogens, with a bacteriostatic rate as high as 95%, and inhibits banana wilt The rate is more than 97%, the inhibition rate to Penicillium and Mucor is more than 93%, the inhibition rate to Rhizopus is more than 91%, the inhibition rate to Cladosporium is more than 82%, and the inhibition rate to Botrytis cinerea and paint spot The inhibition rate of bacteria is more than 51%, and the inhibition rate of Alternaria tengella is more than 27%, which has the potential application value of preparing bio-control bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of bacillus megaterium FJW1 and its application in the preparation of antibacterial agents. Background technique [0002] The development and utilization of microbial fertilizers is an important way for the sustainable development of my country's agriculture. At this stage, the environmental pollution caused by the excessive use of pesticides, the resistance of diseases and insect pests, and the food security problems caused by pesticide residues are becoming more and more serious. Microbial pesticides have attracted much attention due to their advantages of green prevention and control, environmental friendliness, promotion of harmonious ecology, and protection of food safety. [0003] Bacillus preparations have attracted more and more attention due to their strong stress resistance and long-term storage. Compared with the vegetative body of bacteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/11
CPCA01N63/00C12N1/205C12R2001/11
Inventor 吴鹏宇陈振民任浩然王巧梅黄丽龚泽奇梁运祥
Owner HUAZHONG AGRI UNIV
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