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Fungus capable of increasing the content of notoginseng saponins and its application

A technology of notoginseng saponin and content, which is applied in the field of plant biology, can solve the problems of increasing the risk of environmental pollution and increasing the content of notoginseng saponin, and achieve the effect of reducing the improvement of the quality of medicinal materials, increasing the content, and facilitating the expansion of industrial production

Active Publication Date: 2021-08-24
SHENYANG PHARMA UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for promoting the growth of Panax notoginseng and improving the yield of medicinal materials due to unscientific application of chemical fertilizers, hormones, pesticides, etc. in the current production of Panax notoginseng, resulting in significant changes in the properties of medicinal materials and increasing the risk of environmental pollution. Cladosporium tenuissimum with increased content of notoginseng saponin and its application

Method used

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  • Fungus capable of increasing the content of notoginseng saponins and its application
  • Fungus capable of increasing the content of notoginseng saponins and its application
  • Fungus capable of increasing the content of notoginseng saponins and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Example 1 Transceiver of Cladosporium Tenuissimum Fermentation Liquid on Sanqi Roundation

[0031] The strain was inoculated in a PDA medium, and inverted in 25-28 ° C for 5-8 days, activated strains, using sterile water to prepare an activated strain into a spore suspension, a microscope, a spore concentration, and said The concentration of spore suspension is: 1.0 × 10 4 -1.0 × 10 8 I / ml. Take the aporeal suspension inoculated in the fermentation medium PDB, 25-28 ° C, 120-150 r / min, in 5-8 days, the fermentation liquid diluted 100 times, spare. The two-year three-year plant is provided by the Pharmaceutical Plant Institute of Yunnan Agricultural Sciences. As the control, three repetitions were repeated each group, and each of the three seventh seven seedlings were repeated, 50 ml per three or seven seedlings. Three-seven samples were collected from 1, 2, and 3 months, respectively, the three or seven samples were collected by CATase, POD enzyme, and SOD enzyme activit...

Embodiment 2

[0036] Example 2 Cladosporium Tenuissimum Fermentation Liquid Extraction Effects for Sanqi Agronomic Index

[0037] The strain was inoculated in a PDA medium, and inverted in 25-28 ° C for 5-8 days, activated strains, using sterile water to prepare an activated strain into a spore suspension, a microscope, a spore concentration, and said The concentration of spore suspension is: 1.0 × 10 4 -1.0 × 10 8 I / ml. Take the pride suspension inoculated in the fermentation medium PDB, 25-28 ° C, 120-150 r / min, cultured for 5-8 days, extracted three times with an equal volume of ethyl acetate, collected concentrated extract, diluted 100 times, spare. The two-year three-year plant is provided by the Pharmaceutical Plant Institute of Yunnan Agricultural Sciences. As the control, three repetitions were repeated each group, and each of the three seventh seven seedlings were repeated, 50 ml per three or seven seedlings. The three-month sample was collected by three months after figs. Determin...

Embodiment 3

[0041] Example 3 Cladosporium Tenuissimum Fermentation Liquid on the content of three or seven saponins

[0042] The strain was inoculated in a PDA medium, and inverted in 25-28 ° C for 5-8 days, activated strains, using sterile water to prepare an activated strain into a spore suspension, a microscope, a spore concentration, and said The concentration of spore suspension is: 1.0 × 10 4 -1.0 × 10 8 I / ml. Take the aporeal suspension inoculated in the fermentation medium PDB, 25-28 ° C, 120-150 r / min, in 5-8 days, the fermentation liquid diluted 100 times, spare. The two-year three-year plant is provided by the Pharmaceutical Plant Institute of Yunnan Agricultural Sciences. As the control, three repetitions were repeated each group, and each of the three seventh seven seedlings were repeated, 50 ml per three or seven seedlings. Saponin content was collected 3 months after figs. Sanqi Total Saponins and Five Monomer Saponin Content Determination Referring to the first part of "Ch...

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Abstract

The invention relates to a fungus Cladosporium tenuissimum which has a beneficial effect on the notoginseng and can increase the content of notoginseng saponins and its agent, belonging to the technical field of plant biology. The strain was isolated from the stem of healthy biennial Panax notoginseng, and was identified as Cladosporium. The strain was preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number was CGMCC No.14126. The preparation of the bacterial agent mainly includes the pure culture of microorganisms, the preparation of the spore suspension, the production of the bacterial agent and the application of the bacterial agent. The bacterial agent can also be mixed with organic fertilizers to make bacterial fertilizer for use. The bacterial preparation prepared by the invention not only has a beneficial effect on the notoginseng, but also can promote the increase of the saponin content of the notoginseng to a certain extent, showing good ecological application prospects.

Description

Technical field: [0001] The present invention relates to a Cladosporium Tenuissimum and its application thereof, specifically involved in transward fungi, which has benefited from three or seven growth, and its application in improving the content of the three or seven saponins, a plant Biotechnology field. Background technique: [0002] Panax Notoginseng (Burk.), The alias "Tian Sanqi", "Gold Does Not", for the 5th Chemicals, the perennial medicine, homologous herb, "Compendium of Materia Medica", root, stem In the drug, it has the effect of promoting blood circulation and swelling, swelling and pain, is a traditional bulletum market in my country's medicinal materials market, the market demand is large, and the development and utilization value is high. The three seventh mainly contains saponins, flavonoids, three- andstein (amino acids), volatile oil, polysaccharide, etc., wherein the Damarane saponins is its main component, with strong biological activity. It has now been sep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/30A01P21/00A01G7/06C05F11/08C12R1/645
CPCA01G7/06A01N63/30C05F11/08C12N1/14C12N3/00C12N1/145C12R2001/645
Inventor 翟航章朦玥张怡轩张金渝杨天梅杨绍兵
Owner SHENYANG PHARMA UNIVERSITY
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