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Chlorpyrifos pesticide degradation strain, inocula and preparation method thereof

A technology for chlorpyrifos pesticides and degrading bacteria, applied in the field of microorganisms, can solve the problems such as no degrading bacterial agents of chlorpyrifos pesticides are found, and achieve the effects of promoting the coordination and unification of pesticides and food safety for pest control, low production cost and good degradation effect.

Inactive Publication Date: 2008-04-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chlorpyrifos is a pesticide that has been widely used in recent years, and no degrading bacteria specifically targeting chlorpyrifos pesticides have been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Preparation method and growth status determination of chlorpyrifos pesticide degrading bacteria Hu-01

[0034] Activate the strain of chlorpyrifos pesticide-degrading bacteria Hu-01 on a petri dish, and inoculate it on the slant of the test tube. Prepare Czapek medium according to the following formula: sodium nitrate: 2.0g, potassium chloride: 0.5g, magnesium sulfate: 0.5g, dipotassium hydrogen phosphate: 1.0g, iron sulfate: 0.01g, sucrose: 30.0g , Distilled water: 1000mL. Insert 0.1 g of wet bacteria into the liquid Charpy's medium at pH 7.0, and culture in a shaker at 28°C and 150 r / min for 1-7 days.

[0035] The relationship between the culture time and the growth status of the degrading bacteria Hu-01 is shown in Table 1.

[0036] Table 1 The influence of culture time on the dry weight of mycelia of chlorpyrifos pesticide-degrading bacteria

[0037] Mycelium dry weight (g / 50mL)

[0038] Note: The above results are the average of 3 repetiti...

Embodiment 2

[0040] (1) Activate the strain of chlorpyrifos pesticide-degrading fungus Hu-01 on a petri dish, and measure the degradation performance, and inoculate it on the test tube slope for subsequent use;

[0041] (2) The test tube seeds were inoculated in 1000 mL shake flasks containing 250 mL of Chapit's medium, cultured at a constant temperature and shaken to the logarithmic phase, and prepared to inoculate the seed tank;

[0042] (3) The seed tank is 100 liters, the feeding volume is 80 liters, and the medium formula is: NaNO 3 0.2%, K 2 HPO 4 0.1%, KCl 0.05%, MgSO 4 0.05%, Fe2 (SO 4 ) 3 0.001%, 3% sucrose, 0.5% peptone, 1.1kg / cm after feeding 3 Under high pressure, 121°C high-pressure damp heat sterilization, after cooling to 28°C, inoculate the above-mentioned cultured inoculum strains into 100 liters of seed tanks according to the inoculum amount of 8% to 10%, and cultivate them to the logarithmic growth phase. It is 200-300 rpm, and the sterile air ventilation is 45...

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Abstract

The invention discloses a chlorpyrifos pesticide-degrading bacterium which refers to a Hu-01strain pertaining to Cladosporium sp. The preservation number of the strain is CCTCC M207111, and a Genebank accession number of the ITS sequence analysis is EF405864. Meantime, the invention discloses a method of preparation of inocula. The invention has the advantages of low production cost of inocula, convenient operation and good degradation effect. The invention is suitable for being widely used in fruit and vegetable production and export bases in China or in places possessing green food trademark, which has great significances in promoting the production of uncontaminated vegetable and green food.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a chlorpyrifos pesticide-degrading bacterium, its bacterial agent and a preparation method. technical background [0002] Fruits and vegetables are essential necessities of life for people. Pesticides are an indispensable choice for preventing diseases, insect pests and weeds and ensuring yield and quality in the process of fruit and vegetable production. [0003] Ensuring that pesticide residues in fruits and vegetables comply with food hygiene standards, which involves the life and health of every citizen, has become an important part of the "vegetable basket, fruit basket" project implemented by governments at all levels. At the same time, with the continuous improvement of living standards, "green food, pollution-free fruits and vegetables" has become more and more popular among the people, and pesticide residues in fruits and vegetables have also become one of the foc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02
Inventor 胡美英钟国华高燕
Owner SOUTH CHINA AGRI UNIV
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