35 results about "Germ tube" patented technology

The invention belongs to the technical field of microorganisms and particularly relates to a bacterial strain capable of antagonizing botrytis cinerea pathogens after picking of fruits and vegetablesand application of the bacterial strain. The antagonistic bacterium is Bacillus velezensis H1, collected in the China Center for Type Culture Collection (CCTCC) with the collection number of CCTCC NO:M 2019274, is separated from pear fruit surfaces, has very significantly effects of inhibiting botrytis cinerea after picking of fruits and vegetables, has higher inhibition actions on botrytis cinerea, can remarkably inhibit germination of botrytis cinerea spores and growth of germ tubes, has good inhibition functions on the botrytis cinerea after picking of pears, natural rotting after picking of winter jujubes and natural l rotting after picking of grapes, and provides an environmental-friendly, simple and effective way for preservation of fruits and vegetables after picking.

The invention relates to a method for separating and purifying the antibacterial peptide of an endophytic bacteria strain though high efficiency liquid chromatography, and the method comprises the following steps of: preparing the fermentation filter liquor of the strain by taking brevibacillus brevis B-011 as a basic strain, wherein the strain is collected in the China general microbiological culture collection center in April 12, 2013, and the collection number is CGMCC No.7466; adding acetonitrile; uniformly mixing, and standing; centrifugalizing; evaporating and concentrating a supernatant to obtain an antibacterial crude extract; and then carrying out secondary separation by adopting the high efficiency liquid chromatography to obtain an antibacterial peptide activity single-peak component. According to the invention, a bacteriostatic activity substance ( antibacterial peptide) contained in the fermentation filter liquor of the strain B-011 has inhibiting effect on the spore germination of alternaria solani and shows the teratogenic effect on germ tubes and hyphae. The method disclosed by the invention can be used for separating and purifying the bacteriostatic activity component contained in the strain fermentation filter liquor through the high efficiency liquid chromatography and identifying the substance and is conductive to defining the root cause of the strain on inhibiting the alternaria solani and developing the novel antibacterial drug.

The invention discloses a Burkholderia arboria MP5 strain with an antibacterial effect and an application of the Burkholderia arboria MP5 strain. According to the invention, the MP5 strain with an inhibiting effect on plant wilt caused by botrytis cinerea and fusarium oxysporum is obtained through screening; the strain is specifically classified and named as Burkholderia arboria MP5, and is preserved in the China Center for Type Culture Collection, and a preservation number of the strain is CCTCC NO: M 2019702. The experiments on botrytis cinerea and fusarium oxysporum spores and germ tube elongation prove that the MP5 strain obtained by the invention has a strong inhibition effect on botrytis cinerea and fusarium oxysporum, can be prepared into a biocontrol agent for preventing and treating botrytis cinerea and plant fusarium oxysporum, and has a good market application prospect.

Phakopsora pachyrhizi, the fungal pathogen that causes Asian soybean rust, has the potential to cause significant losses in soybean yield in many production regions of the U.S. To assist the development of new modes of soybean resistance to fungal infection, peptides were identified from combinatorial phage-display peptide libraries which inhibit germ tube growth from urediniospores of P. pachyrhizi. Two peptides, Sp2 and Sp39, were identified that inhibit germ tube development when displayed as fusions with the coat protein of M13 phage or as fusions with maize cytokinin oxidase / dehydrogenase (ZmCKX1). These peptides may be used in vitro to help control fungal infection. These peptides may also be expressed as transgenes in a plant to help the resulting transgenic plant defend against the fungi.

Xanthochymol and garcinol, isoprenylated benzophenones purified from Garcinia xanthochymus fruits, showed multiple activities against fungal biofilms. Both compounds effectively prevented emergence of fungal germ tubes and were also cytostatic, with MICs of 1 to 3 ΞM. The compounds therefore inhibited development of hyphae and subsequent biofilm maturation. Xanthochymol treatment of developing and mature biofilms induced cell death. In early biofilm development, killing had the characteristics of apoptosis. These activities resulted in failure of biofilm maturation and hyphal death in mature biofilms. In mature biofilms, xanthochymol and garcinol caused the death of biofilm hyphae, with 50% effective concentrations (EC50s) of 30 to 50 μM. Additionally, xanthochymol-mediated killing was complementary with fluconazole against mature biofilms, reducing the fluconazole EC50 from greater than 1,024 μg per ml to 13 μg per ml.

The invention relates to the technical field of pest control, particularly a method for controlling early blight of greenhouse tomatoes by using an ultraviolet lamp. The invention aims to provide a method for producing pollution-free vegetables according to the actions of control of wavelength radiation dose on tomato early blight spore germination, germ tube extension and hypha growth inhibition. The ultraviolet lamp has great destructive power on deoxyriboneucleic acid (DNA) and ribonucleic acid (RNA) of pathogenic bacteria, and enables the pathogenic bacteria to lose survivability and fertility, thereby destroying the pathogenic bacteria. The method can implement nucleic acid mutation, replication inhibition, transcription blockade and protein synthesis, or can generate free radicals capable of causing photoionization, thereby resulting in cell death and further implementing sterilization. The sterilization efficiency is up to 99%; and the method has the advantages of favorable sterilization effect and high acting speed.

The invention relates to the field of microbiology, and specifically discloses a Lactobacillus brevis 8-2B and an application thereof. Lactobacillus brevis 8-2B of the present invention has a deposit number of CGMCC No.20330. The Lactobacillus brevis 8-2B of the present invention can efficiently inhibit the growth and toxin production of Aspergillus nigricans. It has obvious inhibitory effect on the spore germination and germ tube length of Aspergillus carbonifera. It has good development and application prospects in agricultural product preservation, microbial preparations and other antibacterial related fields, and provides an excellent basic strain for the further research and development of microbial-derived OTA inhibitors.

The present invention discloses a strain of Burkholderia forestae MP5 with antibacterial effect and its application. The present invention obtains strains capable of inhibiting plant wilt caused by Botrytis cinerea and Fusarium oxysporum through screening. MP5 strain, its specific taxonomic designation is Burkholderia arboris MP5, which was deposited in the China Center for Type Culture Collection, and its preservation number is CCTCC NO: M 2019702. The present invention proves that the MP5 bacterial strain obtained by the present invention has a strong inhibitory effect on Botrytis cinerea and Fusarium oxysporum through experiments on Botrytis cinerea and Fusarium oxysporum spores and germ tube elongation, and it can be prepared as a control The biocontrol agent of Botrytis cinerea and Fusarium wilt has a good market application prospect.

The invention provides the application of benzydamine hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria. The present invention proves that benzydamine hydrochloride is effective against tomato cinerea and Fusarium oxysporum mycelia growth, spore germination and germ tube elongation by testing the inhibitory effect of benzydamine hydrochloride on tomato cinerea and Fusarium oxysporum. Fusarium sporogenes tomato specialization has a strong inhibitory effect. Traditional fungicides are highly polluting to the environment and have high residues, which directly threaten food safety. Benzydamine hydrochloride, as a fungicide, has the advantages of high efficiency and low toxicity, and is suitable for the requirements of chemical control of plant diseases. Benzydamine hydrochloride is a non-polluting, environmentally friendly small molecule compound, which is safe for non-target organisms, humans and animals, can ensure the high quality of agricultural products, fruits and vegetables, and meets the requirements of sustainable development. Its research and market application prospects are broad.

The invention relates to a method for separating and purifying the antibacterial peptide of an endophytic bacteria strain though high efficiency liquid chromatography, and the method comprises the following steps of: preparing the fermentation filter liquor of the strain by taking brevibacillus brevis B-011 as a basic strain, wherein the strain is collected in the China general microbiological culture collection center in April 12, 2013, and the collection number is CGMCC No.7466; adding acetonitrile; uniformly mixing, and standing; centrifugalizing; evaporating and concentrating a supernatant to obtain an antibacterial crude extract; and then carrying out secondary separation by adopting the high efficiency liquid chromatography to obtain an antibacterial peptide activity single-peak component. According to the invention, a bacteriostatic activity substance ( antibacterial peptide) contained in the fermentation filter liquor of the strain B-011 has inhibiting effect on the spore germination of alternaria solani and shows the teratogenic effect on germ tubes and hyphae. The method disclosed by the invention can be used for separating and purifying the bacteriostatic activity component contained in the strain fermentation filter liquor through the high efficiency liquid chromatography and identifying the substance and is conductive to defining the root cause of the strain on inhibiting the alternaria solani and developing the novel antibacterial drug.

The invention belongs to the field of plant disease biological control, and discloses the preparation and application of Streptomyces melanogenes and its metabolites. The strain number of the Streptomyces niger is CYS22, and the preservation number of the strain in the General Microbiology Center of the China Microorganism Culture Collection Management Committee is CGMCC No. 18476. The Streptomyces niger producing CYS22 of the present invention has inhibitory effects on various plant pathogenic fungi such as Fusarium oxysporum, oryzae oryzae and rot of apple; the strain CYS22 can effectively prevent and treat Fusarium wilt of balsam pear and rice blast; the results of pot experiments show that , The control effect of Streptomyces niger CYS22 fermentation broth on bitter gourd Fusarium wilt was 60.61%. The present invention prepares an active compound P1 from the metabolite of Streptomyces, which can effectively inhibit the spore germination and germ tube growth of M. oryzae.