34 results about "Appressorium" patented technology

The invention discloses a cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, an extract, a preparation method and an application thereof. The fermentation filtrate of the Epicoccum dendrobii SMEL disclosed by the invention can be used for remarkably inhibiting the colletotrichum gloeosporioides spore germination of the cunninghamia lanceolata and inhibiting the appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; the ethyl acetate extract of the Epicoccum dendrobii SMEL fermentation filtrate can obviously inhibit the mycelial growth ofthe colletotrichum gloeosporioides of the cunninghamia lanceolata, and obviously inhibit the spore germination and appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; and an inoculation test shows that the ethyl acetate extract obviously inhibits the pathogenicity of colletotrichum gloeosporioides of the cunninghamia lanceolata.

The invention discloses a preparation method of a compound agent for prevention and treatment of astragalus powdery mildew. The compound agent comprises the following components by weight: 40-60 partsof a glycolysis solution, 3-8 parts of a surfactant, 0.1-1 part of rosmarinic acid, 0.1-1 part of chitosan, and 0.02-0.04 part of tetrabutyl titanate. The invention has the beneficial effects that: because of the interaction of chitosan and tetrabutyl titanate in the above-mentioned compound agent, the compound agent can form a very thin film on astragalus leaves after use, thus prolonging the action time of the compound agent; rosmarinic acid can improve the killing power of active ingredients in the glycolysis solution to astragalus powdery mildew pathogen; the surfactant can improve the compatibility of the compound agent and astragalus leaves, all the components in the compound agent have coupling effect, can effectively inhibit the spore germination and appressorium formation of astragalus powdery mildew pathogen, and affect the morphology and structure of hypha cells, thus effectively preventing and treating astragalus powdery mildew.

The invention belongs to the production field of industrial seedling culture for aquaculture and particularly relates to egg bag collecting and hatching devices for industrial seedling culture of Rapana venosa and a use method of the devices. The egg bag collecting device comprises attachment devices and a floating frame, the hatching device comprises floating balls, supports and attachment devices, the floating balls are connected to tops of the supports, and the attachment devices and the supports are connected through tail hooks; during egg bag collection, the attachment devices are fixed on the inner wall of the floating frame for culturing parents of Rapana venosa, the attachment devices on the top layer are kept underwater, and the attachment devices with egg bags attached are replaced with new attachment devices with no egg bags attached and then taken out of the floating frame after egg collection; during egg bag hatching, the attachment devices with egg bags attached are hung on the tail hooks of the supports and suspended in a hatching tank for egg bag hatching by the aid of the supports and the floating balls. By means of the devices, the egg bags of the Rapana venosa can be collected conveniently and efficiently and prevented from being damaged due to manual separation of the egg bags, so that the hatching process of the egg bags is finished smoothly.

The invention discloses a new necessary MgPTH12 gene and utility to make pear spore bacterium attach to mature and lead to disease, which is characterized by the following: the gene and its cDNA and coding product possess the sequence in the SEQ ID No.:1, No.:2 and No.:3; the protein of coded gene possesses homeo structural region, which is not similar to known functional protein; the gene removes abnormal and matured attaching cell, which reduces forming frequency of infecting tin and extending infecting hypha without pathogenic ability for rice; the homologous gene of gene lies in the plant pathogenic bacteria widely, which can be applied to design and sieve new drug to prevent fungus.

The invention provides a sanguinarine compound and application thereof in resisting rice blast, the sanguinarine compound exists in the rhizome of opium poppy plant sanguinarine, is a natural alkaloidwith antibacterial, anti-inflammatory and antioxidant properties, and also has the effects of inhibiting tumor cell proliferation and promoting apoptosis. The influence of the sanguinarine compound added in vitro on magnaporthe grisea hypha growth, spore germination, appressorium development and pathogenicity is detected, and the result shows that the expression of rice disease resistance relatedgenes can be effectively activated by adding a certain concentration of sanguinarine to an external source so that the pathogenicity of magnaporthe grisea is remarkably inhibited. The invention provides an inhibition effect of sanguinarine on magnaporthe oryzae pathopoiesis, which indicates that the compound can be used for preventing and treating the magnaporthe oryzae.

The invention discloses the inhibitory activity of polycarboxylic acid compounds on phytopathogenic fungi and oomycete appresses and their application in the prevention and treatment of plant diseases; the polycarboxylic acid compounds are selected from compounds of formula I, their isomers, hydrated food or salt. The polycarboxylic acid compound involved in the present invention can significantly inhibit the formation of appresses of fungi and oomycetes, and can be used for the prevention of various plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora, powdery mildew, etc. , no obvious harm, good safety. Compared with the existing compounds for preventing and controlling plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora, and powdery mildew, the polycarboxylic acid compound of the present invention has good preventive effect, environmental protection, and nontoxicity. , low residue, safety features. Because these compounds are known and widely used compounds, and the environmental toxicology and impact research is thorough, therefore, they have the advantages of convenience, easy availability, environmental protection and safety.

The invention discloses a fermentation filtrate of Chinese fir endophytic fungi and an extract thereof, a preparation method and application thereof. of the present invention Epicoccum dendrobii The fermentation filtrate of SMEL can significantly inhibit the spore germination of Chinese fir anthracis and inhibit the appressorium development of Chinese fir anthracis; Epicoccum dendrobii The ethyl acetate extract of the SMEL fermentation filtrate can significantly inhibit the growth of mycelium of B. fir, and significantly inhibit the spore germination and appressorium development of B. fir. The inoculation test showed that the ethyl acetate extract significantly inhibited the growth of F. anthracis. Sickness.

The invention discloses a specific promoter of pathogenic filamentous fungi. The nucleotide sequence of the specific promoter at least contains a DNA sequence represented by SEQ ID No.1, or a DNA sequence complementary to the sequence represented by the SEQ ID No.1, or a DNA sequence which is 90 or above familiar with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1 and has the same functions with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1. Experiment researches prove that the promoter can be specifically and effectively expressed in a period of appressorium. The promoter provides an important tool for studying the regulation of the pathogenic filamentous fungi in an appressorium formation and permeation into body wall of a host and reconstructing wild insect pathogenic strain gene engineering and has a very high application value.

The invention provides a method for scale breeding of south-migrated stichopus japonicus. The method comprises the steps: selecting mature individuals, which grow for two years in a coastal area, as parental stichopus japonicus, putting the selected parental stichopus japonicus into a breeding pond, and then, stimulating the parental stichopus japonicus to liberate sperms and ova through heating; removing excess male parental stichopus japonicus after heating is ended, then, washing zygotes in the parental stichopus japonicus breeding pond, and hatching in the pond, so as to obtain juvenile; siphoning the juvenile, which moves agilely in an upper layer of water of the breeding pond, to a juvenile breeding pond for juvenile breeding, so as to obtain juvenile stichopus japonicus; attaching the juvenile stichopus japonicus to an attachment device, and suspending the stringed attachment device into a juvenile stichopus japonicus breeding pond, where impurities are discharged, for juvenile stichopus japonicus breeding. According to the method, the problems of high cultivation cost and high death rate caused due to the fact that a large amount of stichopus japonicus seedlings are purchased from the north can be avoided, so that the method provided by the invention is applied to the scale breeding of the south-migrated stichopus japonicus which is suitable for Fujian and neighboring regions thereof.

The invention discloses the application of a blast fungus gene MoRMD1 in regulating the pathogenicity of blast fungus, belonging to the field of plant genetic engineering. The invention constructs a gene knockout vector and introduces it into the protoplast of Magnaporthe oryzae; knocks out the gene MoRMD1 from Magnaporthe oryzae by using a homologous recombination method to obtain a knockout mutant ΔMoRMD1; the obtained Magnaporthe oryzae knockout The mutants are defective in appressorium formation, and MoRMD1 is important for maintaining cell wall integrity. Pathogenicity tests showed that the deletion of MoRMD1 significantly reduced the virulence of Magnaporthe oryzae, and no obvious lesions could be formed on rice leaves. The present invention demonstrates that MoRMD1 is required for appressorium formation, maintenance of cell wall integrity and pathogenicity of M. oryzae. Our research helps to elucidate the pathogenic molecular mechanism of Magnaporthe grisea and provides target genes for the development of effective fungicides.

The invention discloses a preparation method of a pomegranate peel polyphenol botanical fungicide. The pomegranate peel polyphenol botanical fungicide comprises the following components by weight: 40-60 parts of glycolysis liquid, 3-8 parts of a surfactant, 0.1-1 part of pomegranate peel polyphenol, 0.1-1 part of chitosan and 0.02-0.04 part of n-butyl titanate. The invention ahs the beneficial effects that: through the interaction of chitosan and n-butyl titanate in the above-mentioned compound agent, the compound agent can form a layer of very thin film on plant leaves after use, thus prolonging the action time of the compound agent; pomegranate peel polyphenol can improve the killing power of active ingredients in the glycolysis liquid to pathogens; the surfactant can improve the affinity of the compound agent and plant leaves, and all the components in the compound agent have a coupling effect, can effectively inhibit pathogen spore germination and appressorium formation, and affectthe morphology and structure of hypha cells, thus effectively controlling fungal plant diseases.

The invention discloses a medicine for preventing and controlling rice blast fungus. The research of the present invention finds that tangeretin can interfere with the formation of appresses of conidia, has a significant delay effect on the formation of appresses of rice blast fungus, has obvious inhibitory effect on rice blast, and inhibits the pathogenicity of rice blast fungi. It has good application value in the prevention and control of plague and drug development.

The invention discloses a material for inducing appresses of corn leaf spot fungus and its preparation method and application, and belongs to the technical field of research on the infection mechanism of plant pathogenic fungi. The material is made of the following raw materials by volume: 4-6 parts of Si-PUD resin copolymerized with silicone diol and aliphatic isocyanate, and 4-6 parts of self-curing liquid polytetrafluoroethylene resin. The materials for inducing appresses of corn leaf spot fungus of the present invention have safe raw materials, simple preparation, and convenient use, are suitable for high-throughput systems, are used for inducing appresses of corn leaf spot fungi, have high induction efficiency, and are suitable for the infection mechanism of corn leaf spot fungus. Research.