Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sanguinarine-excited rice resistance and application thereof in resisting rice blast

A rice blast, rice blast resistance technology, applied in application, chemical for biological control, animal repellent, etc. Improve the effect of expression

Active Publication Date: 2021-02-05
FUJIAN AGRI & FORESTRY UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies on Magnaporthe grisea have shown that sanguinarine can inhibit spore germination, promote the production of reactive oxygen species, change the morphology of the nucleus and the membrane potential of mitochondria, but its mechanism of action in the infection of Magnaporthe grisea and the defense of rice Not clear, there is no report on the application of sanguinarine in rice blast resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sanguinarine-excited rice resistance and application thereof in resisting rice blast
  • Sanguinarine-excited rice resistance and application thereof in resisting rice blast
  • Sanguinarine-excited rice resistance and application thereof in resisting rice blast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] In the present invention, a certain concentration of sanguinarine is added to the culture medium in vitro, and the effect of sanguinarine on the mycelia growth of the pathogen on the culture medium is observed, and at the same time, a certain concentration of sanguinarine is added to the wild-type strain of Magnaporthe grisea, Guy11 In the conidia suspension of rice blast fungus, observe the appressorium formation of the pathogen on the hydrophobic glass slide, the pathogenic process and the expression of rice resistance response genes, and then determine the rice blast fungus conidia suspension containing sanguinarine. Plague pathogenicity.

[0022] Specifically include the following steps:

[0023] The effect of adding exogenous sanguinarine on the mycelia growth of Magnaporthe grisea: Dissolve sanguinarine with methanol to a concentration of 3.8 mg / mL in the mother solution, add it to the basic medium, and prepare the final concentration of 2.0 μM, 4.0 μM and 8.0 μM...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a sanguinarine compound and application thereof in resisting rice blast, the sanguinarine compound exists in the rhizome of opium poppy plant sanguinarine, is a natural alkaloidwith antibacterial, anti-inflammatory and antioxidant properties, and also has the effects of inhibiting tumor cell proliferation and promoting apoptosis. The influence of the sanguinarine compound added in vitro on magnaporthe grisea hypha growth, spore germination, appressorium development and pathogenicity is detected, and the result shows that the expression of rice disease resistance relatedgenes can be effectively activated by adding a certain concentration of sanguinarine to an external source so that the pathogenicity of magnaporthe grisea is remarkably inhibited. The invention provides an inhibition effect of sanguinarine on magnaporthe oryzae pathopoiesis, which indicates that the compound can be used for preventing and treating the magnaporthe oryzae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of exogenously adding a compound sanguinarine in rice blast resistance. Background technique [0002] Crop diseases caused by pathogenic fungi are one of the serious threats to global food production and food safety. Fungal diseases are the largest category of plant diseases, accounting for about 70-80% of plant diseases. Many plant diseases with wide distribution and serious damage are caused by fungi. Rice is the most important food crop in my country. Nearly 60% of the country's population takes rice as a staple food. It can be seen that the production safety of rice is related to the national economy and people's livelihood of our country. Magnaporthe grisea ( Magnaporthe oryzae ) caused by rice blast is one of the devastating diseases in global rice production. It can occur in the whole development period of rice. %~30% yield loss, which seriously t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 威利汤蔚郑华坤陈美莲郭嘉媛陈奕晓朱尔斯石茗月张冬梅王宗华
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com