43results about How to "Inhibition of pathogenicity" patented technology

The medicine for curing myocarditis is characterized by that it is an oral liquor or intravenous injection made up by using total saponin extracted from Chinese medicinal materials sweetvetch root and sterile physiological saline or phosphoric acid buffer solution through a certain preparation process. It possesses the obvious effect for resisting myocarditis virus and inhibiting pathopoiesis of Coxsackie B3 virus cell, as compared with virazole it possesses obvious protection action for myocarditis, and has no any toxic side effects.

The invention provides an immunogenic molecular entity, a supramolecular assembly, and an antibody that can be used to inhibit Gram-positive bacterial quorum sensing, prevent infection or development of a disease condition associated with a Gram-positive bacterial infection. The invention also provides methods of inhibiting Gram-positive bacterial quorum sensing, and methods of preventing infection or development of a disease condition associated with a Gram-positive bacterial infection.

The invention provides a compound sanguinarine and its anti-rice blast function. The compound exists in the rhizome of Papaveraceae Sanguinarum, and is a natural alkaloid with antibacterial, anti-inflammatory and anti-oxidative properties. Inhibit tumor cell proliferation and promote apoptosis. The present invention detects its effects on the mycelial growth, spore germination, appressorium development and pathogenicity of Magnaporthe grisea by adding sanguinarine compounds in vitro, and the results show that adding a certain concentration of sanguinarine from exogenous sources can effectively activate rice disease resistance-related genes expression, thereby significantly inhibiting the virulence of Magnaporthe grisea. The invention proposes the inhibitory effect of sanguinarine on the pathogenicity of rice blast fungus, indicating that the compound can be used for preventing and treating rice blast.

The invention discloses a Rhodopseudomonas palustris Atps2 protein and its preparation method and application, and belongs to the technical field of biological pesticides; the Rhodopseudomonas palustris Atps2 protein is composed of The ATPS2 gene of Rhodopseudomonas palustris was prokaryotically expressed in Escherichia coli and obtained through protein purification; the Rhodopseudomonas palustris LY-6 strain was preserved in the China Center for Type Culture Collection, The deposit number is CCTCC NO: M2014525, and the deposit date is November 5, 2014. Aiming at the problem that the existing biopesticides do not effectively prevent and control the rice blast fungus, the present invention provides a Rhodopseudomonas palustris Atps2 protein and its preparation method and application, which can effectively inhibit the rice blast fungus.

The invention relates to the use of an adsorbent to reduce or suppress the pathogenicity or virulence of Clostridium bacteria in vitro, ex vivo or in vivo. The invention may be used in any mammal or environment, and is particularly effective to suppress the pathogenicity or virulence of Clostridium difficile.

The invention belongs to the field of biomedicines and in particular relates to a compound for preventing and controlling candida albicans as well as a preparation method and application thereof. Thecompound provided by the invention is 3-methyl-N-(2'-phenemyl)butyramide; an actinomyces streptomyces olivaceus SCSIO T05deltarsdK2 / deltaxmcP mutant strain is constructed through in-frame deletion; amplified fermentation is carried out through a mutant strain fermentation tank, and bacterium collection and centrifuging are carried out; part of mycelia are subjected to ultrasonic extraction throughacetone and part of supernatant is extracted through butanone; extract is combined according to an HPLC (High Performance Liquid Chromatography) analysis result; the compound is obtained by separating through various column chromatography methods. Compared with the prior art, the compound provided by the invention has the beneficial effects that the technology disclosed by the invention successfully prepares and screens the compound 3-methyl-N-(2'-phenemyl)butyramide which has the effects of inhibiting the adhesion of the candida albicans, the mycelium shape transformation and the pathogenicity; meanwhile, the compound 3-methyl-N-(2'-phenemyl)butyramide has relatively small toxicity, does not influence the growth of the candida albicans and human cells and is hopeful to promote and propelthe development of treatment of novel anti-fungus medicines.

The invention discloses a bovine-derived single-chain antibody against Staphylococcus aureus and its preparation and application, and relates to the field of single-chain antibody genetic engineering. The single-chain antibody at least comprises a bovine antibody light chain variable region (VL), a bovine antibody heavy chain variable region (VH) and an intermediate connecting peptide (Linker), and the connection sequence is VL-Linker-VH; its preparation method comprises obtaining bovine antibody Derived single-chain antibody gene, construct primary library of bovine-derived single-chain antibody, enrich and panning, construct single-chain antibody prokaryotic expression plasmid pGEX‑4T‑1‑scFv, obtain single-chain antibody prokaryotic expression protein; can be applied to the treatment of golden yellow Bovine mastitis caused by staphylococcus. The single-chain antibody of the present invention can specifically bind to the LukE protein of Staphylococcus aureus, inhibit LukED from lysing the membrane of bovine mammary epithelial cells, thereby weakening the adhesion and damage of Staphylococcus aureus to bovine mammary epithelial cells, and has a certain inhibitory effect on bovine mammary epithelial cells. Function of staphylococci in mammary gland injury.

The invention discloses a traditional Chinese medicine composition used for preventing and treating mycoplasmal pneumonia of swine. The traditional Chinese medicine composition comprises the following components in parts by weight as main active components: 20-30 parts of herba houttuyniae, 15-25 parts of membranous milkvetch roots, 15-25 parts of radix stemonae, 15-20 parts of platycodon grandiflorum, 15-20 parts of garlic, 10-20 parts of rhizoma anemarrhenae, 10-15 parts of ephedra, 10-15 parts of roots of redroot gromwell, and 5-10 parts of radix glycyrrhizae. The invention also discloses a preparation method and application of the traditional Chinese medicine composition. Tests show that the traditional Chinese medicine composition can inhibit in-vitro growth of mycoplasma hyopneumoniae, can well control the mycoplasma hyopneumoniae for infection and pathogenicity of mice, and is clinically used for preventing and treating infection and pathogenicity of the mycoplasma hyopneumoniae, has a good pharmaceutical effect and no toxic or side effects, and is convenient to take.

The invention aims to disclose the application of allicveratrine in the preparation of medicines for preventing and treating Candida albicans infection. Through the detection of various indicators such as the effect of the compound allicveratrine on the growth of Candida albicans, mycelia inhibition rate, cytotoxicity, and animal models, the results show that allicveratrine has no obvious effect on the growth of Candida albicans, among which, The compound allicilveratrine can still effectively inhibit the mycelium formation of Candida albicans when the concentration is 50 μM, and the mycelium formation rate is lower than 50%. In addition, allicveratrine has a good protective effect on human cell A549 infected by Candida albicans, and the compound allicveratrine itself has no obvious toxic and side effects on cells. In the mouse oral model, garlic veratrine has a good inhibitory effect on the invasion of Candida albicans.

A pharmaceutical composition comprising at least 0.1 μmol of formaurindicarboxylic acid or its derivatives in 1 kg of pharmaceutically acceptable carrier. The pharmaceutical composition of claim 1 wherein the composition is in the form of solution prepared using aqueous alcali or water.

The invention belongs to the technical field of biomedicines and in particular relates to a 2-alkylamine quinoline compound for preventing and treating candida albicans as well as a preparation methodand application of the 2-alkylamine quinoline compound. The compound is prepared from a compound A (1,2,3,4-Tetrahydroquinolines) and a compound B (O-benzoyl hydroxylamines) as raw materials throughsynthesis. Compared with the prior art, the compound has the advantages and effects that results of former research of the inventor show that a diffusible signal factor (DSF) quorum sensing signal anda derivative thereof are capable of relatively intensely interfering conversion of a yeast state to a hypha state of candida albicans, and the compound capable of inhibiting adhesion, hypha state conversion and pathogenicity of the candida albicans is successfully synthesized and screened in the research. Meanwhile, the compound is relatively small in toxicity, growth of the candida albicans andhuman cells is not affected, and the characteristics have the potential of promoting development of novel antifungal drugs.

The invention discloses an R.solani AG1-IA pathogenic related gene and application thereof. The invention provides the R.solani AG1-IA pathogenic related gene and a silent target gene segment Rstpp ofthe R.solani AG1-IA pathogenic related gene. By taking the segment as a target, the pathogenic related genes can be silenced, so that the pathogenicity of R.solani AG1-IA is remarkably inhibited. ThedsRNA of the silent target gene segment Rstpp can be used for preparing a product for preventing and treating R.solani AG1-IA. On the basis, the invention also provides a recombinant vector constructed by using dsRNA. The recombinant vector can be transformed to a plant, and the obtained transgenic plant has obvious disease resistance to R.solani AG1-IA. Therefore, the R.solani AG1-IA pathogenicrelated gene and the silent target gene segment Rstpp thereof have wide popularization and application prospects in prevention and treatment of R.solani AG1-IA including construction of R.solani AG1-IA resistance transgenic plants.