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Application of 9-phenanthrenol in prevention and treatment of rice blast

A rice blast fungus and phenanthroline technology, which is applied to the application field of 9-phenanthroline in rice blast prevention and control, can solve problems such as applications that have not been reported, achieve good application value and form delaying effects

Active Publication Date: 2022-04-01
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of 9-phenanthrene in agricultural production has not been reported

Method used

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  • Application of 9-phenanthrenol in prevention and treatment of rice blast
  • Application of 9-phenanthrenol in prevention and treatment of rice blast
  • Application of 9-phenanthrenol in prevention and treatment of rice blast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Effect of 9-phenanthrene on the growth of blast fungus mycelia

[0020] A method utilizing 9-phenanthrene to suppress the growth of blast fungus mycelia, comprising the following steps:

[0021] S1: Dissolve 9-phenanthreneol in dimethyl sulfoxide, make it into a mother solution (5000ug / ml), add it into PDA medium so that the final concentrations of 9-phenanthreneol are 1.25μg / ml, 2.5μg / ml , 5 μg / ml, 10 μg / ml and 15 μg / ml, the temperature of the PDA medium was controlled at 55°C; meanwhile, the PDA medium without adding 9-phenanthrol was set as a blank control group.

[0022] The preparation method of above-mentioned PDA culture medium:

[0023] (1) Take weighing paper and weigh 10g of sucrose and 15g of agar powder in the balance;

[0024] (2) Peel and clean the potatoes, weigh 200g of the peeled potatoes and cut them into cubes of about 1cm with a knife on a clean gauze;

[0025] (3) Measure 1000mL of distilled water, add the distilled water and 200g of pe...

Embodiment 2

[0042] Example 2 Effect of 9-phenanthreneol on spore formation and appressoria formation of Magnaporthe grisea

[0043] Implementation steps:

[0044] S1 Put the 9-phenanthrene-containing PDA medium and the blank control PDA medium that were put into the bacterial block into a constant temperature incubator and cultivate them for 7 days, then use a sterile puncher to cut the bacterial block on the blast fungus, the diameter of the bacterial block 6mm, pick three bacterial blocks and place them in the PDB medium,

[0045] The preparation method of described PDB medium:

[0046] (1) Take weighing paper and weigh 10g of sucrose in the balance;

[0047](2) Peel and clean the potatoes, weigh 200g of the peeled potatoes and cut them into cubes of about 1cm with a knife on a clean gauze;

[0048] (3) Measure 1000mL of distilled water, add the distilled water and 200g of peeled potato pieces into the pot together, cook for 20-30min after the water boils, then filter the potato resi...

Embodiment 3

[0058] Example 3 Effect of 9-phenanthrene on the pathogenicity of blast fungus

[0059] The experimental steps are as follows:

[0060] Add the 9-phenanthrene phenol mother liquor to the conidia suspension of Magnaporthe oryzae until the final concentration is 10ug / ml and 30ug / ml, and spray the spore suspension evenly on the rice leaves so that the leaves are covered with droplets, and keep in the dark at 28°C Moisturize for 24 hours, then move to the greenhouse, investigate the blast fungus disease 7 days after inoculation and count the mycelial biomass by fluorescence quantitative method, the specific formula 2 C T (Os -UBQ)- C T (Mo -Pot2).

[0061] Result: Rice was inoculated with the conidia suspension containing 9-phenanthrol, and the incidence of rice was observed 7 days after inoculation. Going up lesion significantly reduces, after inoculation added 30ug / ml 9-phenanthrenol to process conidia suspension, rice blade can only see sporadic little lesion ( Figure 5...

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Abstract

The invention discloses application of 9-phenanthrenol in prevention and treatment of rice blast. Experiments show that the compound can effectively inhibit mycelial growth and spore germination of magnaporthe oryzae, can interfere conidia to form appressorium, has an obvious inhibition effect on magnaporthe oryzae, and inhibits pathogenicity of the magnaporthe oryzae, so that the compound has a very good application value in the aspects of magnaporthe oryzae prevention and control and drug development.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of 9-phenanthrene in the prevention and treatment of rice blast. Background technique [0002] Rice (Oryza sativa L.) is one of the important food crops, which occupies a very important position in food production. Nearly 50% of the world's population uses it as a staple food. Its safe production is crucial to ensuring social stability and economic development . Like other crops, rice is inevitably attacked by a series of pathogenic bacteria during its growth and development, which seriously affects the yield and quality of rice. One of the serious diseases is a major problem in rice production in the world. It can reduce rice production by 10% to 30% every year. This disease can occur in all rice planting areas and in different growth stages of rice. It has the characteristics of wide distribution and great harm. This has led to a serious reduction in ric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/08A01P3/00A01G13/00
Inventor 王一李成云刘立娜吴奇浦鑫汪子怡马婵
Owner YUNNAN AGRICULTURAL UNIVERSITY
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