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Inhibitory activity of polycarboxylic acid compounds on fungi and oomycete appressorium and application of polycarboxylic acid compounds in prevention and treatment of plant diseases

A plant disease and compound technology, applied in plant growth regulators, botanical equipment and methods, applications, etc., can solve problems such as agricultural production losses, achieve less residue, prevent pathogens from infecting plants, and achieve precise control effects

Active Publication Date: 2021-02-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, downy mildew of various melons and grapes, late blight of potato and tomato, and phytophthora of pepper, all of which can cause huge losses to agricultural production

Method used

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  • Inhibitory activity of polycarboxylic acid compounds on fungi and oomycete appressorium and application of polycarboxylic acid compounds in prevention and treatment of plant diseases
  • Inhibitory activity of polycarboxylic acid compounds on fungi and oomycete appressorium and application of polycarboxylic acid compounds in prevention and treatment of plant diseases
  • Inhibitory activity of polycarboxylic acid compounds on fungi and oomycete appressorium and application of polycarboxylic acid compounds in prevention and treatment of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 The inhibition of polycarboxylic acid compounds on the formation of appresses of anthracnose bacteria

[0036]1. Bacteria to be tested: 20 anthrax strains in total, namely: grape anthracnose, sorghum anthracnose, camellia oleifera, apple anthracnose, pear anthracnose, strawberry anthracnose, capsicum oxysporum, capsicum anthracnose , deep split bamboo root seven (8270) anthracnose bacteria, deep split bamboo root seven (8069) anthrax bacteria, bovine anthracnose bacteria, huanghuali anthracnose bacteria, cucumber anthracnose bacteria, Luo Han Guo anthracnose bacteria, red camellia (9053) anthracnose bacteria, Red camellia (9059) anthracnose bacteria, cherry anthracnose bacteria, cruciferous vegetable anthracnose bacteria, walnut anthracnose bacteria, corn anthracnose bacteria.

[0037] 2. Test method:

[0038] 1) Anthracis bacteria produce conidia in large quantities: spot the anthrax bacteria strain to be activated on the potato agarose medium PDA, and place...

Embodiment 2

[0050] Embodiment 2 polycarboxylic acid compound is to the inhibition of blast fungus appressorium formation

[0051] 1. Bacteria to be tested: Magnaporthe oryzae P131.

[0052] 2. Test method:

[0053] 1) Magnaporthe oryzae produces a large number of conidia: Spot the to-be-activated Magnaporthe oryzae strain on the tomato oat plate OTA, and place it in a constant temperature light incubator at 28°C for cultivation. After 3-5 days, the colonies that grow on the surface of the petri dish are sufficient. The colonies on the OTA were fully interrupted, and then spread evenly on a new oatmeal plate with tomato juice, and cultured in a constant temperature and light incubator at 28°C. When the new hyphae can be seen growing out of the surface of the culture medium with the naked eye, gently interrupt the mycelium with a cotton swab, rinse it with water, and dry it. Cover the petri dish with a single layer of gauze, and culture it under light at 28°C for about 48 hours, that is,...

Embodiment 3

[0061] Inhibition of embodiment 3 polycarboxylic acid compound on the formation of appressorium of rubber spore YN42

[0062] 1. Bacteria to be tested: Colletotrichum acutatum YN42.

[0063] 2. Test method:

[0064] 1) Anthracis bacteria produce conidia in large quantities: spot the anthrax bacteria strain to be activated on the potato agarose medium PDA, and place it in a constant temperature light incubator at 28°C for cultivation. After 3-5 days, the colonies that grow on the surface of the petri dish are sufficient. Wash off all the hyphae on the surface of the culture medium with sterilized water, rinse them, dry them in the air, and cultivate them under light at 28°C for about 48 hours, and a large number of conidia can be seen on the surface of the PDA.

[0065] 2) Preparation of anthrax spore suspension: wash the spores on the spore-forming plate with sterile water, filter with three layers of filter paper, count with a hemocytometer, and adjust the concentration to ...

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Abstract

The invention discloses inhibitory activity of polycarboxylic acid compounds on plant pathogenic fungi and oomycetes appressorium and an application of the polycarboxylic acid compounds in preventionand treatment of plant diseases. The polycarboxylic acid compound is selected from a compound shown as a formula I, and an isomer, hydrate or salt thereof. The polycarboxylic acid compound disclosed by the invention remarkably inhibits the formation of appressorium of fungi and oomycetes, can be used for preventing various plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora root rot and powdery mildew, and is free of obvious phytotoxicity and good in safety. Compared with an existing compound for preventing and treating plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora root rot and powdery mildew, the polycarboxylic acid compound has the advantages of being good in prevention effect, environmentally friendly, free of toxicity, low in residue and safe. The compounds are known and widely used, and environmental toxicology and influence research is thorough, so that the compounds have the advantages of convenience, easiness in obtaining, environmental protection and safety.

Description

technical field [0001] The invention relates to a new application of polycarboxylic acid compounds, in particular to the activity of this type of compound in inhibiting the formation of appresses of fungi and oomycetes and its application in preventing and controlling plant diseases. Background technique [0002] The polycarboxylic acid compound shown in formula I is a class of known compounds, which are widely used in the fields of chemical industry, food, medicine, materials, textile industry, cosmetics, electronics, metallurgy and the like. For example, representative substances in this class of compounds such as compound citric acid in formula II can be used for carbonated beverages, fruit juice drinks and lactic acid beverages as food additives; using citric acid can also improve soil environment, improve soil quality, and reduce planting production costs , improve planting output and product quality; citric acid also has a certain antibacterial effect, combined with hi...

Claims

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Application Information

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IPC IPC(8): A01N37/36A01N37/04A01P3/00
CPCA01N37/36A01N37/04
Inventor 彭友良张溪郭红超赵文生倪汉文窦道龙王晓丹
Owner CHINA AGRI UNIV
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